Reactions of superoxide dismutases with HS−/H2S and superoxide radical anion: An in vitro EPR study

Bolić, Bojana; Mijušković, Ana; Popović‐Bijelić, Ana; Nikolić‐Kokić, Aleksandra; Spasić, Snežana; Blagojević, Duško; Spasić, Mihajlo; Spasojević, Ivan

doi:10.1016/j.niox.2015.09.008

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…The colorization by H 2 S exposure and decolorization which could be accelerated by the mentioned heating procedure can also be investigated by EPR spectroscopy and can be explained by the reduction of the Cu cations in the framework from Cu(II) to Cu(I). The reduction of copper based complexes [18] or enzymes [19] was already observed by other groups and measured for example by EPR spectroscopy.…”

Section: Exposure To H 2 Ssupporting

confidence: 54%

Cu‐MOF‐808 as a Sensing Material for Gaseous Hydrogen Sulfide

2023

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The selective detection of hydrogen sulfide (H 2 S) is an important task because of its potentially hazardous effects not only on the environment but also on human health. Here, we present the zirconium-based MOF-808, which was modified with copper salts, as a colorimetric sensor material for the visible, reversible and sensitive H 2 S detection at room temperature. The copper cations, which are incorporated in the oxidation state + II, are installed at the inorganic building units (IBUs) of the framework so that they are accessible for interactions with H 2 S. The activated copper doped MOF-808 powder shows significant and fast color change in the presence of H 2 S, which can be detected by UV-vis spectroscopy. Moreover, the detection process is reversible by heating the material to 120 °C under ambient conditions leading to its decolorization. The detection performance of the material was studied by in situ UV-vis measurements in a reaction chamber. The material was able to respond to 100 ppm H 2 S in several cycles of exposure and heating to 120 °C under moist air conditions in a defined wavelength range. This reversibility is very uncommon for copper-based H 2 S sensing reactions and highlights the potential of MOFs as selective sensing materials.

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Section: Exposure To H 2 Ssupporting

confidence: 54%

Cu‐MOF‐808 as a Sensing Material for Gaseous Hydrogen Sulfide

2023

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“…Thiol compounds bind with substantial avidity to soft metals and hemes and therefore are potentially inhibitory to enzymes that use these cofactors (Nies, 2003, Wu et al, 2015, Vitvitsky et al, 2015, Bolic et al, 2015, Nagy, 2015). Zinc has a moderate affinity for thiols, and zinc enzymes can be inhibited when moderate concentrations of cysteine or sulfide coordinate and perhaps extract the metal (Martin & Maricle, 2015, Gu & Imlay, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Like cysteine, sulfide is chemically reactive and potentially problematic. It exhibits a substantial affinity for heme and for soft metals, including those in proteins (Wu et al , 2015, Vitvitsky et al , 2015, Bolic et al , 2015, Nagy, 2015). Due to its tiny size, sulfide cannot easily be physically excluded from their active sites.…”

Section: Introductionmentioning

confidence: 99%

The cytochrome bd oxidase of Escherichia coli prevents respiratory inhibition by endogenous and exogenous hydrogen sulfide

Korshunov

Imlay

2016

Molecular Microbiology

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Summary When sulfur compounds are scarce or difficult to process, E. coli adapts by inducing the high-level expression of sulfur-compound importers. If cystine then becomes available, the cystine is rapidly overimported and reduced, leading to a burgeoning pool of intracellular cysteine. Most of the excess cysteine is exported, but some is adventitiously degraded, with the consequent release of sulfide. Sulfide is a potent ligand of copper and heme moieties, raising the prospect that it interferes with enzymes. We observed that when cystine was provided and sulfide levels rose, E. coli became strictly dependent upon cytochrome bd oxidase for continued respiration. Inspection revealed that low-micromolar levels of sulfide inhibited the proton-pumping cytochrome bo oxidase that is regarded as the primary respiratory oxidase. In the absence of the back-up cytochrome bd oxidase, growth failed. Exogenous sulfide elicited the same effect. The potency of sulfide was enhanced when oxygen concentrations were low. Natural oxic-anoxic interfaces are often sulfidic, including the intestinal environment where E. coli dwells. We propose that the sulfide resistance of the cytochrome bd oxidase is a key trait that permits respiration in such habitats.

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“…H 2 S is a reducing agent and a weak acid with approximately 4:1 HS − /H 2 S ratio at physiological pH [28]. HS − , as opposed to H 2 S, can reduce the metal center of cytochrome c and lead to production of O ⋅− 2 from molecular oxygen [29]. On the other hand, HS − /H 2 S can reduce Cu 2+ to Cu 1+ and enhance O ⋅− 2 scavenging activity of the copper-zinc SOD (CuZnSOD) [29,30].…”

Section: Discussionmentioning

confidence: 99%

“…HS − , as opposed to H 2 S, can reduce the metal center of cytochrome c and lead to production of O ⋅− 2 from molecular oxygen [29]. On the other hand, HS − /H 2 S can reduce Cu 2+ to Cu 1+ and enhance O ⋅− 2 scavenging activity of the copper-zinc SOD (CuZnSOD) [29,30]. Sulfide is a potent reversible inhibitor of myeloperoxidase activity [31].…”

Section: Discussionmentioning

confidence: 99%

Expression and activity of hydrogen sulfide generating enzymes in murine macrophages stimulated with lipopolysaccharide and interferon-γ

et al. 2019

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Murine macrophages of the J774A.1 line are hydrogen sulphide-producing cells with the primary role of γ-cystathionase (CTH) and secondary role of 3-mercaptopyruvate sulfurtransferase (limited by cysteine availability) and with a negligible role of cystathionine β-synthase (CBS) in H 2 S generation. J774A.1 cells stimulation with lipopolysaccharide (LPS) or interferongamma (IFNγ) resulted in decreased H 2 S levels after 24 h of incubation; however, they were restored to the control level after 48 h. Negligible CBS expression and activity in J774A.1 cells can result in homocysteine availability for CTH-catalyzed, H 2 S-generating reactions. This was supported by an increased CTH expression (IFNγ, 24 h and 48 h, and LPS, 48 h) and activity (24 h, LPS) in the stimulated cells. The results confirm the suggested feedback regulation between CBS and CTH.

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Reactions of superoxide dismutases with HS−/H2S and superoxide radical anion: An in vitro EPR study

Cited by 8 publications

References 43 publications

Cu‐MOF‐808 as a Sensing Material for Gaseous Hydrogen Sulfide

Cu‐MOF‐808 as a Sensing Material for Gaseous Hydrogen Sulfide

The cytochrome bd oxidase of Escherichia coli prevents respiratory inhibition by endogenous and exogenous hydrogen sulfide

Expression and activity of hydrogen sulfide generating enzymes in murine macrophages stimulated with lipopolysaccharide and interferon-γ

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