2020
DOI: 10.3389/fchem.2020.581648
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Reactions of Medicinal Gold(III) Compounds With Proteins and Peptides Explored by Electrospray Ionization Mass Spectrometry and Complementary Biophysical Methods

Abstract: Electrospray ionization mass spectrometry (ESI MS) is a powerful investigative tool to analyze the reactions of metallodrugs with proteins and peptides and characterize the resulting adducts. Here, we have applied this type of approach to four experimental anticancer gold(III) compounds for which extensive biological and mechanistic data had previously been gathered, namely, Auoxo6, Au 2 phen, AuL12, and Aubipyc. These gold(III) compounds were reacted with two representative proteins, i.e., human serum albumin… Show more

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Cited by 47 publications
(46 citation statements)
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“…During the past years, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) has emerged as a valuable and powerful tool to study the formation of adducts derived from the interaction of metallodrugs with biomolecules [ 76 , 77 , 78 ]. Moreover, this technique allows the identification of the precise nature of the fragments attached to protein side chains and, thus, to infer some relevant mechanistic information [ 79 , 80 , 81 ].…”
Section: Resultsmentioning
confidence: 99%
“…During the past years, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) has emerged as a valuable and powerful tool to study the formation of adducts derived from the interaction of metallodrugs with biomolecules [ 76 , 77 , 78 ]. Moreover, this technique allows the identification of the precise nature of the fragments attached to protein side chains and, thus, to infer some relevant mechanistic information [ 79 , 80 , 81 ].…”
Section: Resultsmentioning
confidence: 99%
“…Both mechanisms may lead to bacterial cell death. The nucleophilic displacement of bromide in gold(III) complexes by free cysteine residues of proteins has been established by electrospray ionization – mass spectrometry (ESI-MS) studies ( Massai et al, 2020 ).…”
Section: Introductionmentioning
confidence: 99%
“…The reactivity behaviour of complexes 1 – 3 towards the two model proteins was also evaluated using high-resolution ESI mass spectrometry. For these experiments, the proteins were treated in physiological-like conditions, using a well-established protocol [ 18 , 36 , 37 ] that can assure a protein reactivity as similar as possible to that of the physiological environment. Moreover, the spectra were acquired with a methodology able to preserve the protein in its native state, conserving unaltered all the covalent interactions formed in solution and most of the non-covalent ones [ 38 ].…”
Section: Resultsmentioning
confidence: 99%
“…The consensus on the crucial role of the binding of metallodrugs with protein targets has grown [ 14 , 15 ]. Some of us were already involved in the study of metallodrugs interaction with small molecular models mimicking the protein coordinative sites [ 16 , 17 ] and with proteins of bigger size [ 18 , 19 , 20 ].…”
Section: Introductionmentioning
confidence: 99%