Reaction Pattern of Bacillus cereus D-11 Chitosanase on Chitooligosaccharide Alcohols

Gao, Xing-Ai

doi:10.4014/jmb.0805.306

Cited by 10 publications

(7 citation statements)

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“…Thus far, the main products of most of the reported chitosanases such as enzymes from Aspergillus sp. W-2 [25], Bacillus cereus GU-2 [26], Bacillus cereus D-11 [27], have been a mixture of DP2–DP5. These mixtures were quite hard to separate.…”

Section: Resultsmentioning

confidence: 99%

Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39

et al. 2019

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Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides.

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Section: Resultsmentioning

confidence: 99%

Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39

et al. 2019

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“…(GlcN) 7 was cleaved into (GlcN) 3 plus (GlcN) 4 [subsequently, (GlcN) 4 →(GlcN) 2 + (GlcN) 2 ]. The purified endochitosanase (41-kDa) from Bacillus cereus D-11 hydrolyzed chito-oligomers (GlcN) 5–7 into chitobiose, chitotriose and chitotetraose as the final products [85]. Minimal size of the oligosaccharides for enzymatic hydrolysis was pentamer.…”

Section: Bioproduction Of Chitosan Oligosaccharides and Its Monomermentioning

confidence: 99%

“…The monomer GlcNor GlcNOH was never detected from the enzyme reaction. These results suggest that D-11 chitosanase recognizes three glucosamine residues in minus position and simultaneously two residues in plus position from the cleavage point [85]. …”

Section: Bioproduction Of Chitosan Oligosaccharides and Its Monomermentioning

confidence: 99%

Bioproduction of Chitooligosaccharides: Present and Perspectives

2014

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Chitin and chitosan oligosaccharides (COS) have been traditionally obtained by chemical digestion with strong acids. In light of the difficulties associated with these traditional production processes, environmentally compatible and reproducible production alternatives are desirable. Unlike chemical digestion, biodegradation of chitin and chitosan by enzymes or microorganisms does not require the use of toxic chemicals or excessive amounts of wastewater. Enzyme preparations with chitinase, chitosanase, and lysozymeare primarily used to hydrolyze chitin and chitosan. Commercial preparations of cellulase, protease, lipase, and pepsin provide another opportunity for oligosaccharide production. In addition to their hydrolytic activities, the transglycosylation activity of chitinolytic enzymes might be exploited for the synthesis of desired chitin oligomers and their derivatives. Chitin deacetylase is also potentially useful for the preparation of oligosaccharides. Recently, direct production of oligosaccharides from chitin and crab shells by a combination of mechanochemical grinding and enzymatic hydrolysis has been reported. Together with these, other emerging technologies such as direct degradation of chitin from crustacean shells and microbial cell walls, enzymatic synthesis of COS from small building blocks, and protein engineering technology for chitin-related enzymes have been discussed as the most significant challenge for industrial application.

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“…The COSs were checked by thin layer chromatography (TLC) (Jo et al, 2003). The amount of COSs in the reaction mixture was also analyzed by HPLC (model LC-10 AD, Shimadzu, Japan) (Gao et al, 2009).Amounts of oligosaccharides produced were calculated by comparing peak areas with those of the authentic chitosan oligomers.…”

Section: Methodsmentioning

confidence: 99%

Preparation of Chitooligosaccharides from Chitosan using Crude Enzyme of Bacillus cereus D-11

Gao¹,

Zhang²,

Park³

et al. 2012

Journal of Applied Biological Chemistry

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In order to enzymatically produce chitooligosaccharide using the crude enzyme preparation from Bacillus cereus D-11, we first studied the optimal reaction conditions. It was found that the optimal temperature for hydrolysis of chitosan was 55 o C. The ratio of enzyme/substrate should not be lower than 0.13 U/mg in the reaction mixture. The enzyme activity was stable below 50 o C. The products of enzymatic reaction were analyzed by both thin layer chromatography and high performance liquid chromatography. Under the appropriate condition, chitosan was hydrolyzed using the enzyme preparation. The resulting chitooligosaccharides were purified and separated by Dowex (H +) ion exchange chromatography. From 4 g soluble chitosan, 0.95 g (GlcN) 2 , 1.43 g (GlcN) 3 , and 1.18 g (GlcN) 4 were recovered.

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Reaction Pattern of Bacillus cereus D-11 Chitosanase on Chitooligosaccharide Alcohols

Cited by 10 publications

References 0 publications

Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39

Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine Bacterium Pseudoalteromonas sp. SY39

Bioproduction of Chitooligosaccharides: Present and Perspectives

Preparation of Chitooligosaccharides from Chitosan using Crude Enzyme of Bacillus cereus D-11

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