Reaction of Various Experimental Animals to Exposure to Acetylcholine or Histamine, and Morphological Observations of Bronchial Smooth Muscle

Mikami, Hiroki; Inoue, Akifumi; Nishibata, Ryoji; Namura, Shogo; Ino, Takayoshi; Kawamoto, Yasuo

doi:10.1538/expanim1978.37.3_269

Cited by 5 publications

(4 citation statements)

References 2 publications

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“…Furthermore, histamine-induced airway contractions may be caused by a decrease in M 2 muscarinic autoreceptors [29]. Therefore, increased airway response to histamine in BHS [2,[22][23][24] may also be due to the dysfunction of M 2 muscarinic autoreceptors. After treatment with M 3 muscarinic receptor antagonist, contractions of tracheal smooth muscle in BHS were significantly greater than those in BHR.…”

Section: Discussionmentioning

confidence: 99%

Effect of M2 and M3 Muscarinic Receptors on Airway Responsiveness to Carbachol in Bronchial-Hypersensitive (BHS) and Bronchial-Hyposensitive (BHR) Guinea Pigs.

et al. 2001

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Abstract:The expression balance of M 2 and M 3 muscarinic receptor subtypes on the pathogenesis of airway hyperresponsiveness was investigated by using two congenitally related strains of guinea pigs, bronchial-hypersensitive (BHS) and bronchial-hyposensitive (BHR). CCh-induced airway responses in vivo and in vitro were investigated by comparing the effects of muscarinic receptor subtype antagonists, and the relative amounts of M 2 and M 3 muscarinic receptor mRNA in tracheal smooth muscle and lung tissue were investigated. After treatment with muscarinic receptor subtype antagonists, the ventilatory mechanics (V T , R aw , and C dyn ) of response to CCh aerosol inhalation were measured by the bodyplethysmograph method. The effects of these antagonists on CCh-induced tracheal smooth muscle contraction were also investigated. The effects of M 2 muscarinic receptor blockade were less but the effects of M 3 muscarinic receptors blockade on the airway contractile responses were greater in BHS than in BHR. In M 3 muscarinic receptor blockades, CCh-induced tracheal contractions in BHS were significantly greater than those in BHR. In tracheal smooth muscle from BHS, the relative amount of M 2 muscarinic receptors mRNA was less but that of M 3 muscarinic receptor mRNA was more than those in BHR. These results suggest that the high ACh level as a consequence of dysfunction of M 2 muscarinic autoreceptors and the excessive effect of M 3 muscarinic receptors on the airway smooth muscle may play an important role in the pathogenesis of airway hyperresponsiveness.

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Section: Discussionmentioning

confidence: 99%

Effect of M2 and M3 Muscarinic Receptors on Airway Responsiveness to Carbachol in Bronchial-Hypersensitive (BHS) and Bronchial-Hyposensitive (BHR) Guinea Pigs.

et al. 2001

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“…The authors [13,15] have already investi gated differences in bronchial sensitivity in various experimental animals and strains of guinea pigs. Based on these results, we atte mpted to breed model animals with constant bronchial hypersensitivity, using those with hyposensitivity as controls, because the diag nosis of human bronchial asthma involves tes ting for bronchial hypersensitivity.…”

Section: Discussionmentioning

confidence: 99%

“…Inhalation, assessment and selective bre eding : Our usual method [13][14][15] was followed. Namely, each guinea pig was placed in a glass receptacle (volume, 101), and exposed to ACh or Hist using a glass nebulizer (nebulizing vo lume, 0. lml/min, particle size 6.2-21.8pm, mean diameter, 14.7 p m, Nippon Shoji Co., Osaka, Japan) connected to a compressor (air delivery, 61/min, Nippon Shoji Co., Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

Selective Breeding of Two Lines of Guinea Pigs Differing in Bronchial Sensitivity to Acetylcholine and Histamine Exposure

Mikami

Nishibata

Kawamoto

et al. 1991

Experimental Animals

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We developed two lines of guinea pigs , one as model animals for bronchial asthma with bronchial hypersensitivity and the other with hyposensitivity as a control. In the last four years, the bronchial hypersensitive line (BHS) and hypo sensitive line (BHR), both derived from Hartley strain guinea pigs , have been selec ted by using bronchial reactivity to acetylcholine and to histamine as parameters . Both lines have reached the F6 generation. The following results were obtained with the two lines : 1) Sib and cous in matings, and mating of selected consangui neous individuals were adopted in breeding BHS and BHR . The breeding started with six families, each, but in the F6 generation the number of families decreased to two in each line. 2) Appearance rates of hyper-or hyposensitivity to acetylcholine and histamine increased with successive generations in both lines, which had been completely separated by the F6 generation. 3) Coefficients of inbreeding in BHS and BHR in the F6 generation ranged from 42% to 45% in the former and 42% in the latter. 4) Heritabilities (h2) of BHS and BHR for the appearance rates of sensitivity to acetylcholine were presumed to be 0.54 in the former and 0 .69 in the latter. 5) No difference in the body weight of 0, 20, and 40 day-old BHS was observed in any generation. On the other hand, the body weight of 20 and 40 day-old BHR tended to decrease with successive generations. 6) Mean litter sizes of BHS and BHR in each of the generations ranged from 2 .24 to 3.47 animals in the former and from 2.63 to 3.38 animals in the latter. Mortality to 40 days after birth in BHS and BHR in each of the generations ranged from 21 .4% to 37.5% in the former and from 14.3 % to 36.5% in the latter.

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“…Experimental animals : Ten to 15 week-old guinea pigs of either sex in F4-F, generation of bronchial hypersensitive line (BHS) and bronchial hyposensitive line (BHR), both devel-oped by ourselves [19][20][21][22], were used in groups of each 5 to 9 animals. In addition, 8 week-old specific pathogen-free (SPF) male Hartley guinea pigs were purchased from Japan SLC, Hamamatsu, Japan, to be used for passive cutaneous anaphylaxis (PCA).…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Two Lines of Guinea Pigs (BHS and BHR) Differing in Bronchial Sensitivity to Acetylcholine and Histamine Exposure

Mikami

Nishibata

Osaki

et al. 1991

Experimental Animals

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Reaction of Various Experimental Animals to Exposure to Acetylcholine or Histamine, and Morphological Observations of Bronchial Smooth Muscle

Cited by 5 publications

References 2 publications

Effect of M2 and M3 Muscarinic Receptors on Airway Responsiveness to Carbachol in Bronchial-Hypersensitive (BHS) and Bronchial-Hyposensitive (BHR) Guinea Pigs.

Effect of M2 and M3 Muscarinic Receptors on Airway Responsiveness to Carbachol in Bronchial-Hypersensitive (BHS) and Bronchial-Hyposensitive (BHR) Guinea Pigs.

Selective Breeding of Two Lines of Guinea Pigs Differing in Bronchial Sensitivity to Acetylcholine and Histamine Exposure

Characteristics of Two Lines of Guinea Pigs (BHS and BHR) Differing in Bronchial Sensitivity to Acetylcholine and Histamine Exposure

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