Reaction of uric acid and 3-N-ribosyluric acid with 1,1-diphenyl-2-picrylhydrazyl

Smith, Robert C.

doi:10.1016/0045-2068(82)90035-9

Cited by 11 publications

(1 citation statement)

References 13 publications

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“…When DPPH • was selected, uric acid was the only compound that could not be tested, as it is not soluble in methanol. A careful literature search showed that uric acid is tested toward DPPH • ( , ) as an aqueous solution, although the radical is dissolved in alcohol. In our study, it was evidenced that uric acid could not be dissolved in an aqueous environment (pure water of 10 MΩ or PB/PBS, at concentrations of 10 and 5 mM) unless a few drops of alkali were added as described in reference handbooks ().…”

Section: Resultsmentioning

confidence: 99%

Use of Reference Compounds in Antioxidant Activity Assessment

Nenadis

Lazaridou

Tsimidou

2007

J. Agric. Food Chem.

143

104

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The choice of reference compounds is examined as a "critical control point" of antioxidant activity assessment. Gallic, caffeic, sinapic, uric, and ascorbic acids, isoeugenol, and Trolox were tested using different redox (FRAP, Folin-Ciocalteu) and radical scavenging (DPPH*, ABTS*+, CBA, ORAC) assays. The ability to chelate transition metals was assessed to support some of the findings. Analytes were also tested in liposomes. On the basis of the findings, we do not recommend uric acid (due to solubility constrains) and ascorbic acid (due to fast degradation kinetics) as references. The behavior of the rest of the compounds could not always be attributed to typical structural characteristics. Selection of suitable reference compounds for in vitro antioxidant activity assays is not an easy task to achieve. The choice of reference compounds has to remain at the convenience of the researchers, with regard to the aim of the study.

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Section: Resultsmentioning

confidence: 99%