Reaction of Porous Silicon with Both End-Functionalized Organic Compounds Bearing α-Bromo and ω-Carboxy Groups for Immobilization of Biomolecules

Abstract

Both end-functionalized (alpha-bromo and omega-carboxy) compounds were first tested for the radical reaction on the silicon-hydride (Si-H) terminated porous silicon (PSi) with/without the presence of diacyl peroxide initiator under microwave irradiation. Then the carboxylic acid monolayers (CAMs) assembled on PSi through the robust Si-C bonds were converted to amino-reactive linker, N-hydroxysuccinimide (NHS)-ester, terminated monolayers. And finally two proteins of bovine serum albumin (BSA) and lysozyme (Lys) were immobilized through amide bonds. The optimum PSi membrane for protein immobilization without collapse, with parameters of porous radii 4-10 nm and depth 0.2-4.6 mum, was prepared from the (100)-oriented p-type silicon wafer. The chemically converted surface products were monitored with Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FESEM).

Covalently derivatized NTA microarrays on porous silicon for multi-mode detection of His-tagged proteins

“…The electrochemical etching was carried out in a 1:3 (V/V) pure ethanol and 40% aqueous HF for 12 min at a current density of 60 mA/cm 2 , similar to our previous report with minor improvement [14]. The freshly prepared PSi bears 5-10 nm nanometer pores, a porous layer thickness of ~1.5 m, and silicon hydride termini on the topmost surface.…”

“…Immobilization of biomolecules on the PSi surface has been reported by us and other groups recently [12][13][14][15]. Most widely-used immobilization strategies including physical adsorption, electrostatic and hydrophobic interactions, and chemical binding have been implanted onto the PSi surface [12][13][14][15][16][17][18].…”

“…Most widely-used immobilization strategies including physical adsorption, electrostatic and hydrophobic interactions, and chemical binding have been implanted onto the PSi surface [12][13][14][15][16][17][18]. These methods share some common disadvantages such as nonspecific adsorption, decrease of biological activity of immobilized protein, and uncontrolled orientation of attachment.…”

Hybrids perfluorosulfonic acid ionomer and silicon oxide membrane for application in ion-exchange polymer-metal composite actuators

“…Compared to that from curve a, the vibration frequency shifts due to the deeply associated OH. According to Beer's law, the amount of Si-H is proportional to its specific peak area in the absorbance mode of FTIR spectrum [28] . Since the water content is an important element for PFSA-based IPMC actuator, the complex absorbance area of OH···O is integrated to experientially evaluate the water content.…”

Monolayer functionalization of silicon micro and nanowires : towards solar-to-fuel and sensing devices

“…138 The latter methods have led to the immobilization of DNA, [138][139][140][141][142] peptides, 143 biotin-avidin sequences, [144][145][146] antibodies, 147 or other proteins. 129,148,149 The desired application then determines the functionality to be used. For example, Qin et al created an alkyne-functionalized monolayer, protected with a trimethylgermanyl group, which could be reacted subsequently with azide-containing mannose molecules in order to capture E. coli bacteria.…”