Reaction of phosphorylase-a with α-d-glucose 1-phosphate and maltodextrin acceptors to give products with degree of polymerization 6–89

Kazłowski, Bartosz; Ko, Yuan-Tih

doi:10.1016/j.carbpol.2014.01.101

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…Also, the maltoheptaose was indicated to change into other molecules with retention times shorter than that of maltoheptaose during HPAEC-PAD analysis (Nagamine & Komae, 1996). In our previous publication distinct differences between maltoheptaose standard and maltoheptaose collected after HPAEC-PAD monitoring (analysis time >120 min) are localized in the ␣-d-glucopyranose protons 2-4 at non-reducing end (Kazłowski & Ko, 2014). Therefore the individual NAOS and AOS were prepared using method 2; nevertheless method 1 still can be used for precise monitoring of NAOS and AOS chain-length distribution with DP ≤ 46.…”

Section: Discussionmentioning

confidence: 98%

“…2). It was a result of structural changes in part of each separated saccharides with DP 1-12 during relatively long analysis (120 min) in alkaline solution and usage of two separation columns: PA1 and PA100 connected in series (Kazłowski & Ko, 2014). For the peaks with DP ≥ 14, the DP was estimated assuming homologous series, adding one neoagarobiose (for NAOS) or one agarobiose (for AOS) unit residue for each peak (Fig.…”

Section: Determining the Dp Of Naos And Aos Products By Hpaec-padmentioning

confidence: 99%

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Monitoring and preparation of neoagaro- and agaro-oligosaccharide products by high performance anion exchange chromatography systems

Kazłowski

Pan

2015

Carbohydrate Polymers

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Section: Discussionmentioning

confidence: 98%

Section: Determining the Dp Of Naos And Aos Products By Hpaec-padmentioning

confidence: 99%

Monitoring and preparation of neoagaro- and agaro-oligosaccharide products by high performance anion exchange chromatography systems

Kazłowski

Pan

2015

Carbohydrate Polymers

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“…Subsequent to this stage, elongation continues, albeit at a slower rate, until amylose chains assemble causing precipitation. [ 166 ] The average DP is dependent on reaction conditions used, typically falling within the range 100–300 (see later). αGP is peculiar among enzymes catalyzing glycosyl transfer in its use of vitamin B6 (pyridoxal 5′‐phosphate) as cofactor in catalysis.…”

Section: Enzymatically Synthesized α‐Glucan Materialsmentioning

confidence: 99%

Bottom‐Up Synthesized Glucan Materials: Opportunities from Applied Biocatalysis

Zhong,

Nidetzky

2024

Advanced Materials

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Linear D‐glucans are natural polysaccharides of simple chemical structure. They are comprised of D‐glucosyl units linked by a single type of glycosidic bond. Noncovalent interactions within, and between, the D‐glucan chains give rise to a broad variety of macromolecular nanostructures that can assemble into crystalline‐organized materials of tunable morphology. Structure design and functionalization of D‐glucans for diverse material applications largely relies on top‐down processing and chemical derivatization of naturally derived starting materials. The top‐down approach encounters critical limitations in efficiency, selectivity, and flexibility. Bottom‐up approaches of D‐glucan synthesis offer different, and often more precise, ways of polymer structure control and provide means of functional diversification widely inaccessible to top‐down routes of polysaccharide material processing. Here we describe the natural and engineered enzymes (glycosyltransferases, glycoside hydrolases and phosphorylases, glycosynthases) for D‐glucan polymerization and show the use of applied biocatalysis for the bottom‐up assembly of specific D‐glucan structures. We further show advanced material applications of the resulting polymeric products and discuss their important role in the development of sustainable macromolecular materials in a bio‐based circular economy.This article is protected by copyright. All rights reserved

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“…Glycogen phosphorylase catalyzes the reversible phosphorolysis of α-(1, 4)-glucans at the non-reducing end, releasing a glucose-1-phosphate (Glc-1-P) [16]. Through the reversible reaction, α-(1, 4)-D-glucosidic linkages can be formed by the phosphorylase-catalyzed glycosylation in the presence of excess Glc-1-P [17,18]. To make this reaction occur, the primer with degree of polymerization (DP) ≥ 4 is required, and a glucose unit is transferred from Glc-1-P to a non-reducing 4-OH terminus of the primer [19].…”

Section: Introductionmentioning

confidence: 99%

Fabrication, Structure and Functional Characterizations of pH-Responsive Hydrogels Derived from Phytoglycogen

Liu

Chen

et al. 2021

Foods

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The pH-responsive hydrogels were obtained through successive carboxymethylation and phosphorylase elongatation of phytoglycogen and their structure and functional characterizations were investigated. Phytoglycogen (PG) was first carboxymethylated to obtain carboxymethyl phytoglycogen (CM-PG) with degree of substitution (DS) at 0.15, 0.25, 0.30, and 0.40, respectively. Iodine staining and X-ray diffraction analysis suggested that the linear glucan chains were successfully phosphorylase-elongated from the non-reducing ends at the CM-PG surface and assembled into the double helical segments, leading to formation of the hydrogel. The DS of CM-PG significantly influenced elongation of glucan chains. Specifically, fewer glucan chains were elongated for CM-PG with higher DS and the final glucan chains were shorter, resulting in lower gelation rate of chain-elongated CM-PG and lower firmness of the corresponding hydrogels. Scanning electron microscope observed that the hydrogels exhibited a porous and interconnected morphology. The swelling ratio and volume of hydrogels was low at pH 3–5 and then became larger at pH 6–8 due to electrostatic repulsion resulting from deprotonated carboxymethyl groups. Particularly, the hydrogel prepared from chain-elongated CM-PG (DS = 0.25) showed the highest sensitivity to pH. These results suggested that phosphorylase-treated CM-PG formed the pH-responsive hydrogel and that the elongation degree and the properties of hydrogels depended on the carboxymethylation degree. Thus, it was inferred that these hydrogels was a potential carrier system of bioactive substances for their targeted releasing in small intestine.

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Reaction of phosphorylase-a with α-d-glucose 1-phosphate and maltodextrin acceptors to give products with degree of polymerization 6–89

Cited by 5 publications

References 30 publications

Monitoring and preparation of neoagaro- and agaro-oligosaccharide products by high performance anion exchange chromatography systems

Monitoring and preparation of neoagaro- and agaro-oligosaccharide products by high performance anion exchange chromatography systems

Bottom‐Up Synthesized Glucan Materials: Opportunities from Applied Biocatalysis

Fabrication, Structure and Functional Characterizations of pH-Responsive Hydrogels Derived from Phytoglycogen

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