Reaction monitoring using NMR

Bernstein, Michael A.

doi:10.1002/mrc.4436

Cited by 6 publications

(6 citation statements)

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“…1 Monitoring the reaction time course under such conditions provides kinetic and structural information on the reagents, intermediates, and nal products, improving the understanding of reaction mechanisms and kinetic properties. 2 Common spectroscopic methods used for this purpose include midinfrared (MIR), 3 Raman, 4 near-infrared (NIR), 5 mass (MS) 2,6 and nuclear magnetic resonance (NMR) [7][8][9][10][11][12] spectroscopies.…”

Section: Introductionmentioning

confidence: 99%

¹H and ¹⁹F NMR in drug stress testing: the case of voriconazole

et al. 2017

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Section: Introductionmentioning

confidence: 99%

¹H and ¹⁹F NMR in drug stress testing: the case of voriconazole

et al. 2017

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“…With certain limitations on molecule size and concentration (≲ 50-100 kDa, ≳ 10-50 µM) 6,7 , solution NMR can monitor any reaction type or molecular class in a wide range of conditions, including unfractionated cell extracts and living cells 8 . The use of NMR to monitor reactions is common in chemistry 9 , and in recent years, NMR has also been used to follow the dynamics of small-scale reaction networks in biology. However, those studies focused on individual molecule classes, i.e.…”

Section: Introductionmentioning

confidence: 99%

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

et al. 2019

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Cellular behavior is controlled by the interplay of diverse biomolecules. Most experimental methods, however, can monitor only a single molecule class or reaction type at a time. We developed an in vitro Nuclear Magnetic Resonance spectroscopy (NMR) approach, which permitted dynamic quantification of an entire "heterotypic" network -simultaneously monitoring three distinct molecule classes (metabolites, proteins, RNA) and all elementary reaction types (bimolecular interactions, catalysis, unimolecular changes). Focusing on an 8-reaction cotranscriptional RNA folding network, in a single sample we recorded over 35 time-points with over 170 observables each, and accurately determined 5 core reaction constants in multiplex. This reconstruction revealed unexpected cross-talk between the different reactions. We further observed dynamic phase-separation in a system of five distinct RNA binding domains in the course of the RNA transcription reaction. Our Systems NMR approach provides a deeper understanding of biological network dynamics by combining the dynamic resolution of biochemical assays and the multiplexing ability of "omics".Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:

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“…Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for the analysis of solution mixtures of small molecules, with applications that include metabolomics and reaction monitoring. [ 1,2 ] The diffusion‐ordered NMR spectroscopy (DOSY) experiment is particularly well suited for mixture analysis. [ 3,4 ] Sometimes referred to as a form of virtual chromatography, it provides separation of the spectra of a mixtures' components, based on their translational diffusion coefficients.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of spatially‐encoded diffusion‐ordered NMR spectroscopy for the analysis of mixtures

Jacquemmoz

Mishra

Guduff

et al. 2021

Magnetic Reson in Chemistry

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Diffusion‐ordered NMR spectroscopy (DOSY NMR) is a widely used method for the analysis of mixtures. It can be used to separate the spectra of a mixture's components and to analyse interactions. The classic implementation of DOSY experiments, based on an incrementation of the diffusion‐encoding gradient area, requires several minutes or more to collect a 2D data set. Spatially‐encoded (SPEN) DOSY makes it possible to collect a complete data set in less than 1 s, by spatial parallelisation of the effective gradient area. While several short descriptions of SPEN DOSY experiments have been reported, a thorough characterisation of its features and its practical use is missing, and this hinders the use of the method. Here, we present the unusual principles and implementation of the SPEN DOSY experiment, an understanding of which is useful to make optimal use of the method. The encoding and acquisition steps are described, and the parameter relations that govern the setup of SPEN DOSY experiments are discussed. The influence of key parameters, including on sensitivity, is illustrated experimentally on mixtures of small molecules. This study should be useful for the setup of SPEN DOSY experiments, which are particularly useful for systems that evolve in time.

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Reaction monitoring using NMR

Cited by 6 publications

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¹H and ¹⁹F NMR in drug stress testing: the case of voriconazole

¹H and ¹⁹F NMR in drug stress testing: the case of voriconazole

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

Optimisation of spatially‐encoded diffusion‐ordered NMR spectroscopy for the analysis of mixtures

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Reaction monitoring using NMR

Cited by 6 publications

References 0 publications

1H and 19F NMR in drug stress testing: the case of voriconazole

1H and 19F NMR in drug stress testing: the case of voriconazole

Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis

Optimisation of spatially‐encoded diffusion‐ordered NMR spectroscopy for the analysis of mixtures

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Product

Resources

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¹H and ¹⁹F NMR in drug stress testing: the case of voriconazole

¹H and ¹⁹F NMR in drug stress testing: the case of voriconazole