Reaction Chemistry Generated by Nanosecond Pulsed Dielectric Barrier Discharge Treatment is Responsible for the Tumor Eradication in the B16 Melanoma Mouse Model

Chernets, Natalie; Kurpad, Deepa S.; Alexeev, Vitali; Rodrigues, Dário B.; Freeman, Theresa A.

doi:10.1002/ppap.201500140

Cited by 64 publications

(50 citation statements)

References 91 publications

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“…While the results seem promising, in animal studies, tumors recurred when treatment was discontinued and there was visible and histological damage in the treatment area [92,98]. In this study, we used stress-inducing regimes of plasma on cells in contrast to those previously reported [56,99].…”

Section: Discussionmentioning

confidence: 99%

Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

Lin

Truong

Patel

et al. 2017

IJMS

166

154

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A novel application for non-thermal plasma is the induction of immunogenic cancer cell death for cancer immunotherapy. Cells undergoing immunogenic death emit danger signals which facilitate anti-tumor immune responses. Although pathways leading to immunogenic cell death are not fully understood; oxidative stress is considered to be part of the underlying mechanism. Here; we studied the interaction between dielectric barrier discharge plasma and cancer cells for oxidative stress-mediated immunogenic cell death. We assessed changes to the intracellular oxidative environment after plasma treatment and correlated it to emission of two danger signals: surface-exposed calreticulin and secreted adenosine triphosphate. Plasma-generated reactive oxygen and charged species were recognized as the major effectors of immunogenic cell death. Chemical attenuators of intracellular reactive oxygen species successfully abrogated oxidative stress following plasma treatment and modulated the emission of surface-exposed calreticulin. Secreted danger signals from cells undergoing immunogenic death enhanced the anti-tumor activity of macrophages. This study demonstrated that plasma triggers immunogenic cell death through oxidative stress pathways and highlights its potential development for cancer immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

Lin

Truong

Patel

et al. 2017

IJMS

166

154

View full text Add to dashboard Cite

show abstract

“…Corresponding mice survival rates were also strongly increased compared with the control group without the CAP treatment [46]. A very recent study on the anti-melanoma effect of DBD further confirmed that the CAP-treated melanoma tissue completely disappeared at the 22rd day after the treatment [90]. …”

Section: The Anti-cancer Effect Of Cap In Vivomentioning

confidence: 99%

“…One recent study using a mouse xenograft model demonstrated that the increase of temperature on the CAP-treated tumor tissue in vivo is negligible [90] confirming previous reports [46]. Though the contribution of electric field generated by CAP to the anti-cancer capacity of CAP was found to be small [90], further study of this effect and its importance is warranted [95]. Overall, the effect of physical and chemical factors on the in vivo application is still largely unknown.…”

Section: The Anti-cancer Effect Of Cap In Vivomentioning

confidence: 99%

Cold atmospheric plasma, a novel promising anti-cancer treatment modality

2016

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Over the past decade, cold atmospheric plasma (CAP), a near room temperature ionized gas has shown its promising application in cancer therapy. Two CAP devices, namely dielectric barrier discharge and plasma jet, show significantly anti-cancer capacity over dozens of cancer cell lines in vitro and several subcutaneous xenograft tumors in vivo. In contrast to conventional anti-cancer approaches and drugs, CAP is a selective anti-cancer treatment modality. Thus far establishing the chemical and molecular mechanism of the anti-cancer capacity of CAP is far from complete. In this review, we provide a comprehensive introduction of the basics of CAP, state of the art research in this field, the primary challenges, and future directions to cancer biologists.

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“…It resulted in cell cycle arrest and consequently in apoptosis induction [42]. This strategy is particularly promising in the context of tumor treatment [42], as was already described on melanoma tumor model [33]. Similar conclusions were made, observing the role of ROS in the wound healing process [43].…”

Section: Discussionmentioning

confidence: 60%

“…[29–32]. Also the role of intercellular ROS generated in the skin tissue as a result of plasma treatment and their involvement in skin damage extension should not be overlooked [33]. As a result of diffusion of these species, the surrounding tissue is inflamed.…”

Section: Discussionmentioning

confidence: 99%

Safety aspects of atmospheric pressure helium plasma jet operation on skin: In vivo study on mouse skin

et al. 2017

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Biomedical applications of plasma require its efficacy for specific purposes and equally importantly its safety. Herein the safety aspects of cold plasma created with simple atmospheric pressure plasma jet produced with helium gas and electrode discharge are evaluated in skin damage on mouse, at different duration of exposure and gas flow rates. The extent of skin damage and treatments are systematically evaluated using stereomicroscope, labelling with fluorescent dyes, histology, infrared imaging and optical emission spectroscopy. The analyses reveal early and late skin damages as a consequence of plasma treatment, and are attributed to direct and indirect effects of plasma. The results indicate that direct skin damage progresses with longer treatment time and increasing gas flow rates which reflect changes in plasma properties. With increasing flow rates, the temperature on treated skin grows and the RONS formation rises. The direct effects were plasma treatment dependent, whereas the disclosed late—secondary effects were more independent on discharge parameters and related to diffusion of RONS species. Thermal effects and skin heating are related to plasma-coupling properties and are separated from the effects of other RONS. It is demonstrated that cumulative topical treatment with helium plasma jet could lead to skin damage. How these damages can be mitigated is discussed in order to provide guidance, when using atmospheric pressure plasma jets for skin treatments.

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Reaction Chemistry Generated by Nanosecond Pulsed Dielectric Barrier Discharge Treatment is Responsible for the Tumor Eradication in the B16 Melanoma Mouse Model

Cited by 64 publications

References 91 publications

Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

Cold atmospheric plasma, a novel promising anti-cancer treatment modality

Safety aspects of atmospheric pressure helium plasma jet operation on skin: In vivo study on mouse skin

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