Reaction-Based Semiconducting Polymer Nanoprobes for Photoacoustic Imaging of Protein Sulfenic Acids

Lyu, Yan; Zhen, Xu; Miao, Yansong; Pu, Kanyi

doi:10.1021/acsnano.6b05949

Cited by 150 publications

(129 citation statements)

References 49 publications

(77 reference statements)

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“…In addition to EVs and liposomes, intelligent organic nanoparticles, such as semiconducting polymer nanoparticles (SPNs), are also available 134, 135, 136, 137, 138. SPNs constitute not only a new generation of organic nanoparticles for sensing and imaging139, 140, 141, 142, 143 but also a noninvasive remote control method with a near‐infrared absorbing property to regulate in vivo gene expression and cellular signals 135, 142, 144.…”

Section: Discussionmentioning

confidence: 99%

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

2018

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Extracellular vesicles (EVs) are ubiquitous nanosized membrane vesicles consisting of a lipid bilayer enclosing proteins and nucleic acids, which are active in intercellular communications. EVs are increasingly seen as a vital component of many biological functions that were once considered to require the direct participation of stem cells. Consequently, transplantation of EVs is gradually becoming considered an alternative to stem cell transplantation due to their significant advantages, including their relatively low probability of neoplastic transformation and abnormal differentiation. However, as research has progressed, it is realized that EVs derived from native‐source cells may have various shortcomings, which can be corrected by modification and optimization. To date, attempts are made to modify or improve almost all the components of EVs, including the lipid bilayer, proteins, and nucleic acids, launching a new era of modularized EV therapy through the “modular design” of EV components. One high‐yield technique, generating EV mimetic nanovesicles, will help to make industrial production of modularized EVs a reality. These modularized EVs have highly customized “modular design” components related to biological function and targeted delivery and are proposed as a promising approach to achieve personalized and precision medicine.

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Section: Discussionmentioning

confidence: 99%

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

2018

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“…[5][6][7][8] Such versatile and extraordinary properties have motivated researchers to explore the multifunctional Pdots for biological applications. Significant efforts have recently been focusedo ns ynthesizing new conjugated polymers, [9,10] developing functionalization strategies, [11,12] and designing signal transduction/amplification schemes. [13,14] The usefulness of Pdots in biological applications is strongly dependento nt he quality of the surfacef unctionalization to enables pecific recognition.…”

mentioning

confidence: 99%

Light‐Induced PEGylation and Functionalization of Semiconductor Polymer Dots

et al. 2017

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As emiconductor polymer with side-chain oxetane groups was synthesized to form highly fluorescent, functionalized, andu ltra-stablep olymer dots (Pdots). A facile light-induced strategy covalently links functional polyethylene glycol (PEG) molecules to polymer dots while simultaneously providing functional groups for bioconjugation. The influence of photo-crosslinkable sidechains and PEG molecules on the properties and performance of Pdots was systematically investigated. The Pdots functionalized by photo-crosslinking show specific labeling and negligible non-specific binding as compared to non-functionalized Pdots. The resultsp rovide av iable strategy for nanoparticle assembly and functionalization.Semiconducting polymers have attracted considerable interest in both optoelectronic and biomedical applications over past decades. While they were originally developed for optoelectronic devices,t he adaptation of semiconducting polymers into nanoparticles has yielded ar apidlyi ncreasing number of applicationso ft he polymer dots (Pdots)i nb iomedical theranostics.[1-4] The attractiveness of Pdots foro pticali maging, sensing, and phototherapy is due to their superior characteristics, such as large absorption coefficients, excellent photostability,b iocompatibility,a nd suitability for the efficient generation of either photoluminescence, photoacoustic waves, or reactive oxygen species (ROS).[5-8] Such versatile and extraordinary properties have motivated researchers to explore the multifunctional Pdots for biological applications. Significant efforts have recently been focusedo ns ynthesizing new conjugated polymers, [9,10] developing functionalization strategies, [11,12] and designing signal transduction/amplification schemes. [13,14] The usefulness of Pdots in biological applications is strongly dependento nt he quality of the surfacef unctionalization to enables pecific recognition. Hence, many strategies have been implemented to modifyP dot surfaces with different functional molecules, for example, PEGylated lipids and amphiphilic polymers. [15,16] These functional molecules consist of hydrophobic components and hydrophilic segmentsw ith functional groups (e.g.,p olyethylene glycol,a mines, and carboxylic acids). During the Pdot formation, the hydrophobic segments are physically associated with polymer nanoparticles, while the hydrophilic chains extend partlyi nto the aqueous environment for further bioconjugation. Chiu's group developed ad irect functionalization strategy by modifying the side chain of the conjugated polymer.[17] Pdots formed from such functional polymers would directly have surface reactive groups for bioconjugation. In this strategy,the density of the side-chain functional groupss hould be well controlled to preventt he formationo farelatively loose structure, which can significantly affect nanoparticle stability and fluorescenceb rightness. However, challenges remain in the functionalization of Pdot surfaces for biomedical applications. Ideal semiconducting polymer nanoparticles ...

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“…Protein sulfenic acids are the reversible oxide of cysteine in proteins and are recognized as a post‐translational modification when proteins are exposed to oxidative stress . The in vivo detection of protein sulfenic acids still remains a big challenge.…”

Section: Applications Of Photoacoustic Detectionmentioning

confidence: 99%

“…The in vivo detection of protein sulfenic acids still remains a big challenge. Recently, Pu and co‐workers developed a reaction‐based semiconducting polymer nanoprobes (rSPNs) with NIR absorption for in vivo PAD of protein sulfenic acids . The rSPN2, one of their nanoprobes, had an emission peak at 840 nm and a PA peak at 680 nm.…”

Section: Applications Of Photoacoustic Detectionmentioning

confidence: 99%

Photoacoustic Probes for Molecular Detection: Recent Advances and Perspectives

Zeng

Lin

et al. 2018

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Photoacoustic (PA) imaging (PAI) is a noninvasive and nonionizing biomedical imaging modality that combines the advantages of optical imaging and ultrasound imaging. Based on PAI, photoacoustic detection (PAD) is an emerging approach that is involved with the interaction between PA probes and analytes resulting in the changes of photoacoustic signals for molecular detection with rich contrast, high resolution, and deep tissue penetration. This Review focuses on the recent development of PA probes in PAD. The following contents will be discussed in detail: 1) the construction of PA probes; 2) the applications and mechanisms of PAD to different types of analytes, including microenvironments, small biomolecules, or metal ions; 3) the challenges and perspectives of PA probes in PAD.

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Reaction-Based Semiconducting Polymer Nanoprobes for Photoacoustic Imaging of Protein Sulfenic Acids

Cited by 150 publications

References 49 publications

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

Light‐Induced PEGylation and Functionalization of Semiconductor Polymer Dots

Photoacoustic Probes for Molecular Detection: Recent Advances and Perspectives

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