Reação enzimática ao estresse salino durante a germinação de estilosantes

Abstract

Resumo -O objetivo deste trabalho foi avaliar a influência do NaCl nos sistemas isoenzimáticos durante o estádio inicial de germinação do Stylosanthes guianensis (Aubl.) Sw. Sementes das variedades botânicas canenscens, microcephala, pauciflora e vulgaris foram colocadas para germinar em caixas de plástico, com papel-filtro umedecido com água (controle) e solução de NaCl (134 mmol), em estufa BOD, por 14 horas. Após este período, foi realizada a extração das enzimas glutamato desidrogenase e peroxidase. As enzimas apresentaram comportamentos diferenciados nas quatro variedades quando submetidas ao estresse salino.Termos para indexação: Stylosanthes guianensis, salinidade, leguminosa forrageira, isoenzimas Enzymatic reaction to saline stress during stylosanthes germinationAbstract -The objective of this work was to evaluate the influence of the NaC1 on isoenzyme systems during the initial stage of germination of Stylosanthes guianensis (Aubl.) Sw. Seeds of the botanical varieties canenscens, microcephala, pauciflora and vulgaris were placed to germinate in plastic boxes, with filter paper moistened with water (control) and NaC1 solution(134 mmol), in BOD greenhouse, for 14 hours. After this period, the extraction of glutamate dehydrogenase ensymes and peroxidase was carried out. The enzymes presented differentiated behaviors in the four varieties when subjected to the saline stress.

Monitoring of cell wall modifications during callogenesis in Stylosanthes guianensis (Fabaceae) under salt stress conditions

“…Furthermore, organogenic calli have been referred to as a suitable strategy to regenerate stable stylosanthes transgenic plants (Quecini et al 2006). Nevertheless, the studies about behavior under saline conditions in stylosanthes species referred only to germination (Gonela et al 2004, Silva et al 2014) and plant growth (Lovato and Martins 1997;Gonzá-lez et al 2000;Fuentes et al 2010). In this regard, programs of in vitro selection for tolerance to abiotic stresses could be a useful tool; however, this strategy is dependent on the development of efficient and reliable callus induction and plant regeneration systems (Benderradji et al 2007).…”