“…The mixture was kept at 0-4 C for no less than 30 minutes and then the cells were removed by centrifugation. By this pretreatment (Tomizawa, Kasamatsu, Yamaya and Yamada, 1966). 4) FTA : Fluorescent treponemal antibody test : FTA-200 (Deacon Freeman and Harris, 1960) and FTA-ABS (Hunter, Deacon and Meyer 1964) were tried.…”