Re-indentification of Pathogens of Anemone and Prune Anthracnose.

Sato, Toyozo; Ueda, Seinosuke; Iijima, Akihiko; Tezuka, Nobuo

doi:10.3186/jjphytopath.62.170

Cited by 10 publications

(5 citation statements)

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“…Phytoparasitica), and possibly also from Italy (20), as C. gloeosporioides was incorrect. Similarly, it has been reported that previously identified strains of C. gloeosporioides causing anemone anthracnose in Japan are C. acutatum (32). It is especially important to differentiate between genotypes within mixed populations for accurate diagnostics, for chemical control, and for breeding for resistance.…”

Section: Discussionmentioning

confidence: 99%

Characterization ofColletotrichum acutatumCausing Anthracnose of Anemone (Anemone coronariaL.)

Freeman

Shabi

Katan

2000

Appl Environ Microbiol

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Anthracnose, or leaf-curl disease of anemone, caused by Colletotrichum sp., has been reported to occur in Australia, western Europe, and Japan. Symptoms include tissue necrosis, corm rot, leaf crinkles, and characteristic spiral twisting of floral peduncles. Three epidemics of the disease have been recorded in Israel: in 1978, in 1990 to 1993, and in 1996 to 1998. We characterized 92 Colletotrichum isolates associated with anthracnose of anemone (Anemone coronaria L.) for vegetative compatibility (72 isolates) and for molecular genotype (92 isolates) and virulence (4 isolates). Eighty-six of the isolates represented the three epidemics in Israel, one isolate was from Australia, and five isolates originated from western Europe. We divided these isolates into three vegetative-compatibility groups (VCGs). One VCG (ANE-A) included all 10 isolates from the first and second epidemics, and 13 of 62 examined isolates from the third epidemic in Israel, along with the isolate from Australia and 4 of 5 isolates from Europe. Another VCG (ANE-F) included most of the examined isolates (49 of the 62) from the third epidemic, as well as Colletotrichum acutatum from strawberry, in Israel. Based on PCR amplification with species-specific primers, all of the anemone isolates were identified as C. acutatum. Anemone and strawberry isolates of the two VCGs were genotypically similar and indistinguishable when compared by arbitrarily primed PCR of genomic DNA. Only isolate NL-12 from The Netherlands, confirmed as C. acutatum but not compatible with either VCG, had a distinct genotype; this isolate represents a third VCG of C. acutatum. Isolates from anemone and strawberry could infect both plant species in artificial inoculations. VCG ANE-F was recovered from natural infections of both anemone and strawberry, but VCG ANE-A was recovered only from anemone. This study of C. acutatum from anemone illustrates the potential of VCG analysis to reveal distinct subspecific groups within a pathogen population which appears to be genotypically homogeneous by molecular assays.

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Section: Discussionmentioning

confidence: 99%

Characterization ofColletotrichum acutatumCausing Anthracnose of Anemone (Anemone coronariaL.)

Freeman

Shabi

Katan

2000

Appl Environ Microbiol

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“…Appressorial morphology on potato carrot agar has been regarded as one of the criteria for taxonomy and iden- tification of Colletotrichum spp., since Sutton 31 showed morphological variation among the species. That of some representative strains of the three member species, groups A2-P and A4 of the species complex, has already been reported 12,21,22,23,30,36,37 . Its shape appeared to show few differences among the species and groups.…”

Section: Morphologymentioning

confidence: 70%

“…Approximately 50% of 170 strains deposited as C. acutatum were re-identified as C. fioriniae, which reflects the fact that the member species was easily identified as C. acutatum 27 with a reddish colony and fusiform conidia 7,9,21,22,32,38,41,42,43,44 . The strains of C. simmondsii were divided into a few sub-clades, suggesting the polyphyletic nature of the species.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…Its shape appeared to show few differences among the species and groups. Appressorial sizes of each of the species and groups were unified as follows: C. fioriniae: 5.8-14 (-15) × 4-8 (-9.8) μm 21,22,23 , C. simmondsii: 5.4-14 × 4-8 (-9.8) μm 23,37 , C. carthami: 3.7-9.2 (-12.8) × 4.6-6 (-7.4) μm 37 , C. acutatum A2-P: 4-17 × 3.2-8 μm 12,36 and C. acutatum A4: 6.9-15.8 × 4.6-7.8 μm 30 . That of C. carthami inclined to small sides in comparison with those of the others, bearing appressoria of similar size.…”

Section: Morphologymentioning

confidence: 99%

“…colony color and conidial shape, has been reported to vary among strains 1,23 . Because some pathogenic strains of species causing anthracnose resembled Colletotrichum gloeosporioides (Penzig) Penzig & Saccardo, they were often misidentified as the latter species, especially in Japan 21 . A few morpho-logically distinct groups, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Re-identification of Strains of the Colletotrichum acutatum Species Complex Deposited in the NIAS Genebank and Morphological Characteristics of its Member Species

Sato

Moriwaki

Misawa

2013

JARQ

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Recently, nine phylogenetic groups were proposed for a plurivorous fungus, Colletotrichum acutatum by some researchers. Some of them were named as C. simmondsii, C. fioriniae, C. acutatum sensu stricto and C. carthami based on morphology and phylogenetic analyses of β-tubulin-2 gene partial sequences. We re-identified 170 strains deposited as C. acutatum in the NIAS Genebank, Japan based on phylogenetic analysis with the β-tubulin-2 gene sequences. Eighty strains with reddish colony colors and relatively uniform conidia belonged to C. fioriniae, 37 with a grayish to yellowish color and short conidia to C. simmondsii, 25 with a blackish to cream color and short conidia to C. carthami, 12 with a cream to pale orange color and narrow conidia to 'C. acutatum group A2-P' newly designated in this paper, 5 with a brownish to orange color and larger conidia to C. acutatum group A4, and 11 to other species of Colletotrichum. No strain of C. acutatum s. str. was found. A dichotomous key to the member species and groups was designated based on reverse colony color and conidial morphology. Because pathogenic strains of 18 host plants were re-identified, updating of pathogen names was proposed. Ten of them should be changed to C. fioriniae, and the pathogens of each five host plants such as strawberry and apple should be replaced with 2 to 3 species or the group. Sweet pepper pathogen was tentatively classified into C. acutatum group A2-P.

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Colletotrichum carthami comb. nov., an anthracnose pathogen of safflower, garland chrysanthemum and pot marigold, revived by molecular phylogeny with authentic herbarium specimens

et al. 2012

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Re-indentification of Pathogens of Anemone and Prune Anthracnose.

Cited by 10 publications

References 1 publication

Characterization ofColletotrichum acutatumCausing Anthracnose of Anemone (Anemone coronariaL.)

Characterization ofColletotrichum acutatumCausing Anthracnose of Anemone (Anemone coronariaL.)

Molecular Re-identification of Strains of the Colletotrichum acutatum Species Complex Deposited in the NIAS Genebank and Morphological Characteristics of its Member Species

Colletotrichum carthami comb. nov., an anthracnose pathogen of safflower, garland chrysanthemum and pot marigold, revived by molecular phylogeny with authentic herbarium specimens

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