Re-examining ferritin-bound iron: current and developing clinical tools

Grant, Erin S; Clucas, Danielle; McColl, Gawain; Hall, Liam T.; Simpson, David A.

doi:10.1515/cclm-2020-1095

Cited by 11 publications

(9 citation statements)

References 117 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, the minimum detectable iron load that could be measured at this protein concentration, was 101 ± 13 Fe/ferritin which is equivalent to 2.2% of the maximum iron load, making QSR a highly sensitive technique. This value covers the expected range of serum ferritin iron loads which appears to be between 5 and 25%, far less than the holoferritin found in tissue. Additionally, the morphology at the low end of iron loads means that the sensitivity is high within this window, making QSR a good technique for studying serum iron content.…”

Section: Resultsmentioning

confidence: 50%

Nonmonotonic Superparamagnetic Behavior of the Ferritin Iron Core Revealed via Quantum Spin Relaxometry

et al. 2022

Self Cite

View full text Add to dashboard Cite

Ferritin is the primary storage protein in our body and is of significant interest in biochemistry, nanotechnology, and condensed matter physics. More specifically within this sphere of interest are the magnetic properties of the iron core of ferritin, which have been utilized as a contrast agent in applications such as magnetic resonance imaging. This magnetism depends on both the number of iron atoms present, L, and the nature of the magnetic ordering of their electron spins. In this work, we create a series of ferritin samples containing homogeneous iron loads and apply diamond-based quantum spin relaxometry to systematically study their room temperature magnetic properties. We observe anomalous magnetic behavior that can be explained using a theoretical model detailing a morphological change to the iron core occurring at relatively low iron loads. This model provides an L 0.35±0.06 scaling of the uncompensated Fe spins, in agreement with previous theoretical predictions. The necessary inclusion of this morphological change within the model is also supported by electron microscopy studies of ferritin with low iron content. This provides evidence for a magnetic consequence of this morphological change and positions diamond-based quantum spin relaxometry as an effective, noninvasive tool for probing the magnetic properties of metalloproteins. The low detection limit (ferritin 2% loaded at a concentration of 7.5 ± 0.4 μg/mL) also makes this a promising method for precision applications where low analyte concentrations are unavoidable, such as in biological research or even clinical analysis.

show abstract

Section: Resultsmentioning

confidence: 50%

Nonmonotonic Superparamagnetic Behavior of the Ferritin Iron Core Revealed via Quantum Spin Relaxometry

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…In anemic patients with suspected iron depletion states, a low SF provides a definitive diagnosis of IDA [ 27 , 44 ]. In cases with borderline SF, ID is typically confirmed with a panel of markers which may include serum iron, total iron-binding capacity, and transferrin saturation [ 44 , 45 ]. Newer iron status parameters, such as soluble transferrin receptors, reticulocyte Hb content, and serum hepcidin, are promising additions to the panel of diagnostic tests for ID; however, these assays are not widely available and are not adequately standardized [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Prevention and Management of Iron Deficiency/Iron-Deficiency Anemia in Women: An Asian Expert Consensus

Pai,

Chong,

Clemente-Chua

et al. 2023

Nutrients

View full text Add to dashboard Cite

The lack of standardized clinical practice impeding the optimal management of iron deficiency (ID) and iron deficiency anemia (IDA) in women is a global concern, particularly in the Asia-Pacific region. The aim of this study was to determine best practices through a Delphi consensus process. In Round 1, panelists were asked to rate their level of agreement with 99 statements across four domains: identification, diagnosis and assessment, prevention, and treatment of ID/IDA in women. In Round 2, panelists reappraised their ratings in view of the collective feedback and responses to Round 1. After two rounds, consensus (≥85% agreement) was reached for 84% of the Delphi statements. Experts agreed on the role of presenting symptoms and risk factors in prompting assessments of anemia and iron status in women. Experts repeatedly called for prevention, recommending preventive iron supplementation for pregnant women irrespective of anemia prevalence levels, and for non-pregnant adult women, adolescent girls, and perimenopausal women living in areas with a high prevalence of anemia. Experts unanimously agreed to prescribing oral ferrous iron as first-line therapy for uncomplicated ID/IDA. The recommendations and clinical pathway algorithms generated should be used to inform clinical practice and standardize the care of women at risk or presenting with ID/IDA in the Asia-Pacific region.

show abstract

“…Ferritin, the iron storage protein, can also be found in low concentrations in the plasma (between 30 and 300 µg/L for men, between 15 and 150 µg/L for women) and reflects the iron stored in the body [ 9 ]. However, the iron content of ferritin can vary, and there is no accepted method for measuring ferritin-bound iron [ 81 ]. Furthermore, ferritin is an acute-phase protein whose synthesis is increased during inflammation by circulating cytokines such as IL-6, under which conditions serum ferritin may not correlate with the body's iron storage status [ 82 ].…”

Section: Higher Plasma Iron Levels Correlate With Poor Outcomes In Th...mentioning

confidence: 99%

Iron as an emerging therapeutic target in critically ill patients

Grange,

Lux,

Brichart

et al. 2023

Crit Care

View full text Add to dashboard Cite

The multiple roles of iron in the body have been known for decades, particularly its involvement in iron overload diseases such as hemochromatosis. More recently, compelling evidence has emerged regarding the critical role of non-transferrin bound iron (NTBI), also known as catalytic iron, in the care of critically ill patients in intensive care units (ICUs). These trace amounts of iron constitute a small percentage of the serum iron, yet they are heavily implicated in the exacerbation of diseases, primarily by catalyzing the formation of reactive oxygen species, which promote oxidative stress. Additionally, catalytic iron activates macrophages and facilitates the growth of pathogens. This review aims to shed light on this underappreciated phenomenon and explore the various common sources of NTBI in ICU patients, which lead to transient iron dysregulation during acute phases of disease. Iron serves as the linchpin of a vicious cycle in many ICU pathologies that are often multifactorial. The clinical evidence showing its detrimental impact on patient outcomes will be outlined in the major ICU pathologies. Finally, different therapeutic strategies will be reviewed, including the targeting of proteins involved in iron metabolism, conventional chelation therapy, and the combination of renal replacement therapy with chelation therapy.

show abstract

Re-examining ferritin-bound iron: current and developing clinical tools

Cited by 11 publications

References 117 publications

Nonmonotonic Superparamagnetic Behavior of the Ferritin Iron Core Revealed via Quantum Spin Relaxometry

Nonmonotonic Superparamagnetic Behavior of the Ferritin Iron Core Revealed via Quantum Spin Relaxometry

Prevention and Management of Iron Deficiency/Iron-Deficiency Anemia in Women: An Asian Expert Consensus

Iron as an emerging therapeutic target in critically ill patients

Contact Info

Product

Resources

About