Re-evaluation of the Role of Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP) in Cellular Calcium Signaling

Abstract

Background: Calcium homeostasis endoplasmic reticulum protein (CHERP) was originally identified as an integral endoplasmic reticulum membrane protein that regulates intracellular Ca 2ϩ channels. Results: In contrast, we show CHERP binds SR140 in nuclear subdomains. Conclusion: CHERP regulates Ca 2ϩ homeostasis indirectly, not as a binding partner of ryanodine receptors or IP 3 receptors. Significance: These data support an entirely new model for the cellular role of CHERP.

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A Role for Monomethylation of Histone H3-K27 in Gene Activity inDrosophila

“…protocol (RAPPSILBER et al 2003), one-50 th of total samples were analyzed on an LTQ Orbitrap Velos (Thermo Scientific) instrument essentially as described (LIN-MOSHIER et al 2013). …”

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“…The fi rst early fraction was concatenated with the fi rst late fraction, and so on ( 18 ). Concatenated samples were dried in vacuo, resuspended in load solvent (98:2:0.01, water:acetonitrile:formic acid), and 1.5 g aliquots were run on a Velos Orbitrap mass spectrometer (Thermo Fisher Scientifi c, Inc.) as described previously, with the exception that the activation energy was 40 ms ( 19 ).…”