Objective: Some surgeons accept the concept of prophylactic methotrexate immediately post-linear salpingotomy for tubal ectopic pregnancy against odd of persistent trophoblast tissue (PTT); however, to avoid one case of PTT, 8 women would need to be treated with methotrexate. The aim of this study was to ascertain whether methotrexate is required immediately in all cases postoperatively. Methods: Women who underwent linear salpingotomy for unruptured ectopic pregnancy and who agreed to participate in this pilot study were assigned to postpone the prophylactic methotrexate that is given to patients with suspected PTT. PTT was suspected if there was a rise, plateau, or decline of < 50% at 2 days and 75% at 4 days post-surgery in the serum b-human chorionic gonadotrophin (hCG) levels. All women were followed with serial serum b-hCG levels; titers were measured on the 2nd and 4th postoperative days, and then weekly until they become undetectable. Results: A total of 22 women completed the postoperative follow-up. No women had suspected PTT, and methotrexate not given to any of the studied women. Conclusions: In spite of very small number of studied patients, it appears that postponing methotrexate to 2 or 4 days post-surgery can help to reserve this medication for situations in which unsatisfactory decrease in B-hCG levels are detected. ( J GYNECOL SURG 28:275)