Re-evaluation of Cryptosporiopsis eucalypti and Cryptosporiopsis-like species occurring on Eucalyptus leaves

Abstract

Cryptosporiopsis eucalypti is a common follicolous pathogen of Eucalyptus species in tropical and temperate regions where these trees are grown in plantations. The taxonomy of C. eucalypti is confused by the fact that it is phylogenetically unrelated to the type species of Cryptosporiopsis (Cryptosporiopsis nigra = C. scutellata, Helotiales). The aim of this study was to resolve the taxonomic position of C. eucalypti based on morphology and phylogenetic inference. Thirty-two Eucalyptus leaf samples with symptoms typical of C. eucalypti infection were collected from 10 tropical and temperate countries across four continents. Cultures were established from single conidia, as well as from ascospores of a previously unreported teleomorph state. DNA sequences were obtained for the 28 S nrDNA, the internal transcribed spacers of the nrDNA operon, and beta-tubulin regions to determine generic and species-level relationships. DNAsequence analysis showed that conidial and ascospore isolates of C. eucalypti have low intraspecific variation, although two collections from Australia and one from Uruguay represented two novel taxa. Based on the newly collected teleomorph stage, as well as the phylogenetic data, C. eucalypti is shown to represent a new genus closely related to Plagiostoma (Gnomoniaceae, Diaporthales) for which the names Pseudoplagiostoma gen. nov. and Pseudoplagiostomaceae fam. nov. (Diaporthales) are introduced. Two new species of Cryptosporiopsis (Dermateaceae, Helotiales) on Eucalyptus from Australia and California (USA) are also described.

A Destructive New Disease of Citrus in China Caused by Cryptosporiopsis citricarpa sp. nov.

“…Considering the teleomorph of this pathogen has not been found, the anamorphic name Cryptosporiopsis citricarpa is assigned to accommodate it. 12-49 × 4-7 a N. malicorticis (25) 15-35 × 3-6 c 5-8 × 1-1.5 c N. perennans (25) 12-25 × 3-6 d 6-10 × 1.5-3 d N. alba (25) 17-30 × 2.5-3.5 a 15-18 × 0.5-1 a C. kienholzii (17) 12-17.5 × 2.5-3.5 c 2.5-6.5 × 1.5-2.5 c N. krawtzewii (21) 25-45 × 4.5-5 a 4.5-8 × 1.5-2.5 a 25-35 × 4 a C. citri (14) Not related 9-11.5 × 4-5.5 a 9.5-12 × 2.5-5 a C. californiae (5) Not related 15-18 × 4. (Fig.…”

Identification of New Fungal Pathogens of Coast Live Oak in California

“…2A). in Cahfomia are shown in Tables 4 and 5 along with previously reported measurements (6,23,28,44,50,51,53,54). The conidial measurements obtained for these Dermateaceae and Diatrypella spp.…”

“…It bas been determined tbat tbere are few reliable morpbological characters to distinguisb among taxa of Cryptosporiopsis spp., tbougb tbis genus cbaracteristically bas large, ellipsoid, aseptate conidia with a protuberant scar (46,53). (6,53), pycnidia witb cbaracteristic conidia were found in C. querciphila, and sequence data suggest a position witbin the genus Pezicula. (6,53), pycnidia witb cbaracteristic conidia were found in C. querciphila, and sequence data suggest a position witbin the genus Pezicula.…”

“…Eor groups of fungi for which morphology was not clearly matched to a single species description, conidial dimensions were compared with those of previous studies (6,23,28,44,50,51,53,54). Eor groups of fungi for which morphology was not clearly matched to a single species description, conidial dimensions were compared with those of previous studies (6,23,28,44,50,51,53,54).…”

Melansporellaceae: a novel family of Diaporthales (Ascomycota)

“…Kirk et al (2008) added Melogrammataceae and listed ten families in this order, whereas placed Melogrammataceae within Xylariales rather than Diaporthales based on ITS and LSU sequence data. Pseudoplagiostomaceae, Harknessiaceae and Tirisporellaceae were also added to the Diaporthales (Cheewangkoon et al 2010, Crous et al 2012. Voglmayr & Jaklitsch (2014) resurrected Stilbosporaceae, while Togniniaceae was reallocated to the Togniniales (Gramaje et al 2015, Maharachchikumbura et al 2015.…”