Re-Emergence of Very Virulent IBDV in Egypt

Metwally, Ashraf M.; Yousif, Ausama; Shaheed, Iman B.; Mohammed, W. A.; Samy, A. M.; Reda, I. M.

doi:10.3923/ijv.2009.1.17

Cited by 21 publications

(12 citation statements)

References 44 publications

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“…A set of primers were used for the RT-PCR reaction and for the subsequent sequence analysis using forward and reverse PCR primers for amplification of 620 bp fragment IBDV on VP2: Forward primer: 5ʹ-TCA CCG TCC TCA GCT TAC CCA CAT C-3ʹ and Reverse primer: 5ʹ-GGA TTT GGG ATC AGC TCG AAG TTG C-3ʹ. They were manufactured by metabion GmbH, (Lena-Christ-Strasse, Germany) after Metwally et al, (2009). Preparation of 50 μl reaction mixture of 10 μl of extracted template RNA, 10 μl RT-PCR buffer, 2 μl of primer forward and 2 μl of primer reverse, 2 μl of dNTPs master mix containing 400 μM each dATP, dGTP, dCTP, dTTP and 2 μl of Qiagen One Step Enzyme Mix.…”

Section: Identification Of Ibdv Using Reverse Transcription-polymerasmentioning

confidence: 99%

“…Very virulent IBD viruses (vvIBDVs) have been isolated in Asia, Central Europe, Russia, the Middle East, and South America (van den Berg, 2000). In Egypt, both vvIBDV strains and variant IBDV strains were reported and has been a serious problem circulating in flocks vaccinated using classical IBDV vaccines (Metwally et al 2009, Helal et al, 2012, Mohamed et al, 2014and Sara et al, 2014. Diagnosis of IBDV depends on isolation of the suspected virus then serological identification using FAT, ELISA, and AGPT but detection of IBDV using RT-PCR showed superior results and greater sensitivity and specificity than the serological techniques (van den Berg, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and molecular characterization of IBDV from Qualubyia governorate, Egypt, 2015

El-Bagoury¹,

El-Nahas²,

El-Habbaa³

2015

Benha Veterinary Medical Journal

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A B S T R A C TInfectious bursal disease virus (IBDV) remerged frequently in Qualubyia was the cause of serious economic losses. Twenty field samples were collected from broiler chick farms in different localities of the governorate in January 2015, subjected for isolation on specific pathogen free-embryonated chicken eggs (SPF-ECE), detection using AGID and RT-PCR. Ten samples showed positive results with egg isolation, AGID and RT-PCR. Sequence analysis and characterization of the variable region of VP2 gene purified from the PCR product showed many amino acid substitutions that may affect IBDV antigenicity and virulence. Comparative phylogenetic analysis based on the sequence of the hypervariable region of VP2 gene clustered the isolated IBDV at a distance from the other reference IBDV from Egypt, Europe and other vaccinal strains. It was concluded that substitution mutations observed with the hypervariable region of VP2 gene of the isolated IBDV could affect the virus antigenicity and further studies on the efficacy of vaccines used locally against this variant IBDV strain have to be investigated.

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Section: Identification Of Ibdv Using Reverse Transcription-polymerasmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and molecular characterization of IBDV from Qualubyia governorate, Egypt, 2015

El-Bagoury¹,

El-Nahas²,

El-Habbaa³

2015

Benha Veterinary Medical Journal

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“…cDNA was synthesised using one step RT-PCR Kit (Qiagen, Germany) following the manufacturer instructions. The cDNA was further used for the amplification of VP2 gene of IBDV through gene specific forward and reverse primers VP2F5'-TACCGTCCTCAGCTTACCCACATC3' and VP2R5'-GGATTTGGGATCAGCTC GAAGTTGC3' according to Metwally et al (2009). The RT-PCR was performed in 50 µL volumes, the reaction mixture consisted of 25 µL of 2× RT-PCR buffer, 1 µL of forward and reverse primers, 1 µL RT enzyme, 1 µL MgSO 4 , 11 µL RNasefree water and 10 µL RNA template.…”

Section: Rt-pcrmentioning

confidence: 99%

Molecular characterisation of infectious bursal disease virus (IBDV) isolated from commercial broiler chickens in Nile Delta

Alkhalefa¹,

El-Abasy²,

Kasem³

et al. 2019

BJVM

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Infectious bursal disease virus (IBDV) is a highly infectious disease affecting young chickens that alters predominantly the immune system. Emergence of new variants causes severe economic losses not only in Egypt but also all over the world. For this purpose assessment of infectious bursal disease virus (IBDV) genotypes in 20 commercial broiler flocks aged 20–35 days raised in 5 provinces in the Nile Delta, Egypt (Gharbia, Dakahlya, Kafr El sheikh, Zagazig and Domietta) was carried out. All flocks were vaccinated against IBD virus. RT-PCR revealed successful amplification of 620 bp of VP2 in 17 out of 20 samples (85%). VP2 gene nucleotide sequence analysis of six IBDV isolates (F342-1, F342-2, F342-4, F342-5 and F342-7) revealed 99.1 % similarity to the Giza 2000, Giza 2008 vv, SV-G1, SV-G2, SV-G4 and SV-G5 which were very virulent IBDV strains while the isolate F342-3 was close to D78 classical vaccinal strain and Kal 2001 classical IBDV strain variant.

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“…Variant IBD strains were also reported (El-Batrawi and El- Kady, 1990). Circulating IBDV strains were isolated from flocks vaccinated using classical IBDV vaccines (Abdel-Alim et al, 2003, Hussein et al, 2003, Metwally et al 2003, Metwally et al 2009, Helal et al, 2012, Mohamed et al, 2014and Sara et al, 2014. The aim of our study was the isolation and molecular characterization of IBDV recently isolated from broiler flocks in Giza governorate, Egypt.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of IBD virus isolated from Giza governorate, Egypt, 2014

El-Bagoury

El-Habbaa

Elsayed

et al. 2015

Benha Veterinary Medical Journal

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The emergence of new variant strains of the infectious bursal disease virus (IBDV) has been the cause of serious economic losses in chicks. A field isolate of IBDV was recovered from suspected broiler chicks in different localities of Giza governorate in 2014. The virus was isolated on specific pathogen freeembryonated chicken eggs (SPF-ECE), detected by AGPT and confirmed by RT-PCR searching for VP2 coding region of the serotype I IBDV. A total number of 26 out of 42 tested samples (61.9%) were positive for virus isolation, AGPT and RT-PCR. Sequence analysis of the PCR product and genomic characterization of the variable region of VP2 of the field isolate revealed amino acid substitutions in the hydrophilic region A in the loops containing domains important for antigenic variation, binding of neutralizing antibodies and the residues responsible for the viral virulence and cellular tropism. Studying homology and phylogeny of the isolated virus with the other reference classical, very virulent, variant and vaccinal strains of IBDV revealed that IBDV Giza 2014 was in a separate branch and it was clustered more close to the Egyptian very virulent IBDV, Giza 2000 and Giza 2008 and the European and German IBDV but it was clustered at a far distance from Hel 2008 IM, Hel 2008, Bursine Plus and other strains. It was concluded that genomic characterization of a variant IBDV which denote the continuous evolution and mutation of IBDV in Egypt which may affect the virus antigenicity and virulence.

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Re-Emergence of Very Virulent IBDV in Egypt

Cited by 21 publications

References 44 publications

Isolation and molecular characterization of IBDV from Qualubyia governorate, Egypt, 2015

Isolation and molecular characterization of IBDV from Qualubyia governorate, Egypt, 2015

Molecular characterisation of infectious bursal disease virus (IBDV) isolated from commercial broiler chickens in Nile Delta

Molecular characterization of IBD virus isolated from Giza governorate, Egypt, 2014

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