Re-emergence of <i>Tobacco streak virus</i> Infecting Soybean in the United States and Canada

Irizarry, Melissa D.; Groves, Carol L.; Elmore, Manjula G.; Bradley, Carl A.; Dasgupta, Ranjit; German, Thomas L.; Jardine, Douglas J.; Rojas, Erika Saalau; Smith, Damon; Tenuta, Albert; Whitham, Steven A.; Mueller, Daren S.

doi:10.1094/php-br-15-0052

Cited by 8 publications

(8 citation statements)

References 8 publications

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“…The presence of TSV in a coinfection with SVNV in a sample from Alabama in 2016 also represents a first report of TSV in soybean in this state. Both viruses are transmitted by thrips [ 18 , 76 ], and so, it may be expected that conditions favoring thrips could result in frequent co-infections by these viruses. It is interesting that the re-emergence of TSV in soybean was previously reported throughout the Midwest including Iowa, Kansas, and Wisconsin as well as Ontario, Canada [ 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

“…Both viruses are transmitted by thrips [ 18 , 76 ], and so, it may be expected that conditions favoring thrips could result in frequent co-infections by these viruses. It is interesting that the re-emergence of TSV in soybean was previously reported throughout the Midwest including Iowa, Kansas, and Wisconsin as well as Ontario, Canada [ 76 , 77 ]. The re-emergence of TSV in soybean in Iowa in 2013 occurred in several counties and was associated with irregular, black streaks and necrotic areas on pods and plants that tested positive for TSV using ELISA [ 76 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is interesting that the re-emergence of TSV in soybean was previously reported throughout the Midwest including Iowa, Kansas, and Wisconsin as well as Ontario, Canada [ 76 , 77 ]. The re-emergence of TSV in soybean in Iowa in 2013 occurred in several counties and was associated with irregular, black streaks and necrotic areas on pods and plants that tested positive for TSV using ELISA [ 76 ]. TSV was also reported in several counties in Illinois from 2006–2008 [ 78 ] as well as in 2013 [ 76 ] and in Ohio in 2011 and 2012 [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

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Detection and discovery of plant viruses in soybean by metagenomic sequencing

Elmore

Groves

Hajimorad

et al. 2022

Virol J

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Background Viruses negatively impact soybean production by causing diseases that affect yield and seed quality. Newly emerging or re-emerging viruses can also threaten soybean production because current control measures may not be effective against them. Furthermore, detection and characterization of new plant viruses requires major efforts when no sequence or antibody-based resources are available. Methods In this study, soybean fields were scouted for virus-like disease symptoms during the 2016–2019 growing seasons. Total RNA was extracted from symptomatic soybean parts, cDNA libraries were prepared, and RNA sequencing was performed using high-throughput sequencing (HTS). A custom bioinformatic workflow was used to identify and assemble known and unknown virus genomes. Results Several viruses were identified in single or mixed infections. Full- or nearly full-length genomes were generated for tobacco streak virus (TSV), alfalfa mosaic virus (AMV), tobacco ringspot virus (TRSV), soybean dwarf virus (SbDV), bean pod mottle virus (BPMV), soybean vein necrosis virus (SVNV), clover yellow vein virus (ClYVV), and a novel virus named soybean ilarvirus 1 (SIlV1). Two distinct ClYVV isolates were recovered, and their biological properties were investigated in Nicotiana benthamiana, broad bean, and soybean. In addition to infections by individual viruses, we also found that mixed viral infections in various combinations were quite common. Conclusions Taken together, the results of this study showed that HTS-based technology is a valuable diagnostic tool for the identification of several viruses in field-grown soybean and can provide rapid information about expected viruses as well as viruses that were previously not detected in soybean.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Detection and discovery of plant viruses in soybean by metagenomic sequencing

Elmore

Groves

Hajimorad

et al. 2022

Virol J

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“…Among the insects that attack soybean, thrips (Thysanoptera: Thripidae) are considered emerging pests (Agrocampo, 2019;Engel & Pasini, 2020), with reported productivity losses of up to 17% (Gamundi & Perotti, 2009). Thrips cause direct damage, by sucking up cell content and injecting toxins into plants, and indirect damage, as they are a vector of tospoviruses such as Soybean vein necrosis virus and Tobacco streak virus (Han et al, 2019;Irizarry et al, 2016). In the main producing regions, particularly in the Americas and Asia, the management of thrips in soybean crops is mainly based on the application of insecticides by aircraft or tractors .…”

Section: Introductionmentioning

confidence: 99%

Economic injury levels for control decision-making of thrips in soybean crops (Glycine max (L.) Merrill)

Neves

Lopes

Sarmento

et al. 2022

RSD

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Soybean, Glycine max (L.) Merrill, is the most cultivated crop worldwide. Recently, thrips (Thysanoptera: Thripidae) has caused of up to 17% production losses to this crop. To improve its management, economic injury levels (EIL) are key tools in supporting decision-making systems in integrated pest management (IPM) programs. The EIL depend on the technology used for insecticide application. In this regard, pesticide treatment on soybean crops can be successfully performed by aircraft or tractors, especially among major producers (e.g., Brazil, the United States, Argentina, and China). There are currently no economic injury levels (EIL) for thrips in soybean crops. Therefore, the objective of this work was to determine economic injury levels for thrips in soybean, with insecticide applications by aircraft and by tractors. The study was carried out in four commercial soybean crops for two years. Frankliniella schultzei and Caliothrips phaseoli were observed attacking soybean plants. The EIL were 4.53 and 3.43 thrips sample-1 for insecticide application with aircraft and tractor, respectively. The economic injury levels, established in this study, can be used in integrated pest management program for soybean crops.

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“…Sporadic reports of the virus suggest it has not been a widespread problem in commercial soybean fields, or that it has gone underreported. Recently, a re-emergence of TSV has been reported in Oklahoma (Dutta et al 2015), Iowa, and Wisconsin (Irizarry et al 2016). Tobacco streak virus is an understudied virus in soybean and the agronomic impacts on the crop are not fully understood (Rabedeaux et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Specific qPCR and Nested RT-PCR Assays for the Detection of Tobacco Streak Virus in Soybean

et al. 2021

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Tobacco streak virus (TSV) is a re-emerging and understudied pathogen of soybean (Glycine max). Management of TSV is challenging due to the multiple modes of transmission, widespread susceptibility of commercial soybean, and lack of reliable diagnostic tests for the virus. Soybean plants with TSV-like, virus-like, or no symptoms were collected from commercial and research fields in seven counties in Wisconsin. Two sensitive assays were developed for the detection of TSV: a fluorescent dye-based quantitative RT-PCR (qPCR) assay and a nested RT-PCR (nRT-PCR). Tobacco streak virus was detected in 47% and 91% of symptomatic samples using the qPCR assay and the nRT-PCR assay, respectively, suggesting that the nRT-PCR assay has higher sensitivity for detecting TSV. The qPCR assay’s limit of detection was determined at 10 fg and the assay was used to estimate the viral load in TSV-symptomatic samples. The titer of TSV in these samples was determined by absolute quantification and ranged from 15 fg to 0.796 ng. The two assays reported here provide diagnostic tools for the rapid and accurate detection of TSV that can aid in monitoring outbreaks, assessing management strategies, or screening soybean cultivars/accessions for resistance to the virus.

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Re-emergence of Tobacco streak virus Infecting Soybean in the United States and Canada

Cited by 8 publications

References 8 publications

Detection and discovery of plant viruses in soybean by metagenomic sequencing

Detection and discovery of plant viruses in soybean by metagenomic sequencing

Economic injury levels for control decision-making of thrips in soybean crops (Glycine max (L.) Merrill)

Sensitive and Specific qPCR and Nested RT-PCR Assays for the Detection of Tobacco Streak Virus in Soybean

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