Re-activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing

Marhavá, Petra; Hoermayer, Lukas; Yoshida, Saiko; Marhavý, Peter; Benková, Eva; Friml, Jiřı́

doi:10.1016/j.cell.2019.04.015

Cited by 100 publications

(126 citation statements)

References 66 publications

(115 reference statements)

Supporting

Mentioning

125

Contrasting

Order By: Relevance

“…Yet, plants launch organogenesis programme when they suffer wounds. This transcriptomic feature might be explained as an accompanying effect of plant immune system activation [37]. Plants regularly endure wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary abilities to restore their tissues after injuries [38].…”

Section: Discussionmentioning

confidence: 99%

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Dong

Li-zhi

et al. 2019

Genes

View full text Add to dashboard Cite

De novo shoot regeneration is one of the important manifestations of cell totipotency in organogenesis, which reflects a survival strategy organism evolved when facing natural selection. Compared with tissue regeneration, and somatic embryogenesis, de novo shoot regeneration denotes a shoot regeneration process directly from detatched or injured tissues of plant. Studies on plant shoot regeneration had identified key genes mediating shoot regeneration. However, knowledge was derived from Arabidopsis; the regeneration capacity is hugely distinct among species. To achieve a comprehensive understanding of the shoot regeneration mechanism from tree species, we select four genetic lines of Populus euphratica from a natural population to be sequenced at transcriptome level. On the basis of the large difference of differentiation capacity, between the highly differentiated (HD) and low differentiated (LD) groups, the analysis of differential expression identified 4920 differentially expressed genes (DEGs), which were revealed in five groups of expression patterns by clustering analysis. Enrichment showed crucial pathways involved in regulation of regeneration difference, including "plant hormone signal transduction", "cell differentiation", "cellular response to auxin stimulus", and "auxin-activated signaling pathway". The expression of nine genes reported to be associated with shoot regeneration was validated using quantitative real-time PCR (qRT-PCR). For the specificity of regeneration mechanism with P. euphratica, large amount of DEGs involved in "plant-pathogen interaction", ubiquitin-26S proteosome mediated proteolysis pathway, stress-responsive DEGs, and senescence-associated DEGs were summarized to possibly account for the differentiation difference with distinct genotypes of P. euphratica. The result in this study helps screening of key regulators in mediating the shoot differentiation. The transcriptomic characteristic in P. euphratica further enhances our understanding of key processes affecting the regeneration capacity of de novo shoots among distinct species.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Dong

Li-zhi

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…It has been shown that JA activates root stem cells Marhava et al, 2019;Zhou et al, 2019). In addition, JA and ERF109 evoke auxin biosynthesis through ASA1 (Sun et al, 2009;Cai et al, 2014).…”

Section: The Role Of Abr1 In Root Regenerationmentioning

confidence: 99%

“…However, how these signals regulate gene expression in the nucleus is largely unknown. The identification of wound-induced AP2/ERFs suggests the early wound signal rapidly reprograms the transcriptome at the transcriptional level (Marhava et al, 2019;Zhang et al, 2019;Zhou et al, 2019). Intriguingly, studies from the Sugimoto lab have shown that another group of AP2/ERF-type transcriptional factor genes, named WOUND-INDUCED DEDIFFERENTIATION1 (WIND1) and its homologs WIND2, WIND3 and WIND4, is induced upon wounding (Iwase et al, 2011a;2011b).…”

Section: Nature Of the Wound Signal During Plant Regenerationmentioning

confidence: 99%

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

et al. 2019

View full text Add to dashboard Cite

Age and wounding are two major determinants for regeneration. In plants, the root regeneration is triggered by woundinduced auxin biosynthesis. As plants age, the root regenerative capacity gradually decreases. How wounding leads to the auxin burst and how age and wound signals collaboratively regulate root regenerative capacity are poorly understood. Here, we show that the increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, and SPL11, suppress root regeneration with age by inhibiting wound-induced auxin biosynthesis. Mechanistically, we find that a subset of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors including ABSCISIC ACID REPRESSOR1 and ERF109 is rapidly induced by wounding and serves as a proxy for wound signal to induce auxin biosynthesis. In older plants, SPL2/10/11 directly bind to the promoters of AP2/ERFs and attenuates their induction, thereby dampening auxin accumulation at the wound. Our results thus identify AP2/ERFs as a hub for integration of age and wound signal for root regeneration.

show abstract

“…Pattern restoration upon cell-type targeted ablation or wounding has been shown to involve SHR and PLT triggering formative divisions in inner adjacent cells (29). As SHR moves from inside to outside, signaling and activation of regenerative divisions (from wounded cells) might require the existence of unknown positional signaling.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…REPRESSOR OF WUSCHEL1 (ROW1) and a circuit form by CLAVATA3-ESR RELATED 40 (CLE40), CLAVATA1 (CLV1) and ARABIDOPSIS CRINKLY 4 (ACR4) restrict WOX5 expression to the QC within the stem cell niche (25,26). Interestingly, WOX5 protein can move to adjacent distal stem cells to regulate their differentiation state (27) Wounded or ablated cells can be replaced by adjacent meristematic cells, which are highly reprogrammable (28,29) During standard growth, QC cells may also divide to replenish dead stem cells, however, this is restricted by RETINOBLASTOMA-RELATED (RBR) protein / SCR circuit and WOX5 working as repressors of mitotic transitions and CYCLIN D activity, respectively (5,30). Regenerative QC divisions can be induced upon supplementation with genotoxic agents that selectively kill stem cells, showing that the QC may function as a reservoir for stem cells (31,32).…”

Section: Introductionmentioning

confidence: 99%

Root Patterning and Regeneration Are Mediated by the Quiescent Center and Involve Bluejay, Jackdaw and Scarecrow Regulation of Vasculature Factors

Sánchez-Corrionero

Pérez-García

Cabrera

et al. 2019

Preprint

View full text Add to dashboard Cite

Stem cells are central to plant development. Root stem cells organize around the quiescent center (QC) and give rise to cell lineages that generate the root radial pattern during postembryonic development. Here, through analyses of Arabidopsis thaliana mutants in the transcription factors BLUEJAY, JACKDAW and SCARECROW and chemical treatments, we have found that QC regenerative capacities maintain the stem cell niche during postembryonic development; and in addition, specification of QC cells are required to re-establish the radial pattern upon wounding. Our data shows that QC functionality in maintaining and regenerating the radial pattern requires non-cellautonomous regulation by transcription factors BLUEJAY, JACKDAW and SCARECROW. We observe that these factors regulate auxin transport, auxin maxima formation, as well as the stem cell regulator SHORT-ROOT from the ground tissue. We tracked this non-cell-autonomous regulation to C-REPEAT BINDING FACTOR 3 (CBF3). CBF3 protein is mobile, while CBF3 gene is expressed in the ground tissue downstream of BLUEJAY, JACKDAW and SCARECROW. Targeted-expression of CBF3 to the ground tissue of blj jkd scr mutant recovers radial patterning and, upon laser ablation, stem cell niche regeneration and tissue organization in layers. CBF3 also regulates QC organization and inhibits stem cell niche differentiation. We propose CBF3 may function as a radial signal maintaining stem cell niche activity and regeneration.

show abstract

Re-activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing

Cited by 100 publications

References 66 publications

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

Root Patterning and Regeneration Are Mediated by the Quiescent Center and Involve Bluejay, Jackdaw and Scarecrow Regulation of Vasculature Factors

Contact Info

Product

Resources

About