RDRio Mycobacterium tuberculosis strains associated with isoniazid resistance in Northern Mexico

Bocanegra-García, Virgilio; Cortez-de-la-Fuente, Luis Jesús; Nakamura-López, Yuko; González, Gloria M.; Rivera, Gildardo; Palma-Nicolás, José Prisco

doi:10.1016/j.eimce.2020.07.010

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…In this study, lineage 4 (Euro-American) was the most common (~96%) lineage identi ed, consistent with previous reports that have shown that isolates with this lineage are predominant in Mexico [18]. We were also able to observe that isolates with LAM lineage (163), the proportion of RD Rio was 69.9%, higher compared to the 63.1% recently described in isolates from Northern Mexico and in isolates from Venezuela (55%), Argentine (11%) and Paraguay (10%) [19][20]. Therefore, our results support that these lineages are endemic and that strains spread regionally with different rates of distribution.…”

Section: Discussionsupporting

confidence: 90%

“…The LAM RD Rio lineage has been described in other Latin American countries where it has been associated with the presence of cavitations, increased transmissibility and MDR [19]. However, in the present study we observed more proportion of LAM RD Rio isolates in cured patients, previously in this study population was obtained that previous treatment (aOR 9.05, 95% CI 3.6-22.5, p < 0.001) and LAM lineage (aOR 4.25, 95% CI 1.4-12.7, p = 0.010) were associated with tuberculosis MDR [15].…”

Section: Discussionmentioning

confidence: 99%

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Mycobacterium tuberculosis lineage 4 associated with cavitations and treatment failure

Ordaz-Vázquez

Torres-González

Ferreyra-Reyes

et al. 2022

Preprint

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Background Mycobacterium tuberculosis genotyping has been crucial to determining the distribution and impact of different families on disease clinical presentation. The aim of the study was to evaluate the associations among sociodemographic and clinical characteristics and M. tuberculosis lineages from patients with pulmonary tuberculosis in Orizaba, Veracruz, Mexico. Methods We analyzed data from 755 patients whose isolates were typified by 24-loci mycobacterial interspersed repetitive unit–variable number of tandem repeats (MIRU-VNTR). The associations among patient characteristics and sublineages found were evaluated using logistic regression analysis. Results Among M. tuberculosis isolates, 730/755 (96.6%) were assigned to eight sublineages of lineage 4 (Euro-American). Alcohol consumption (adjusted odds ratio [aOR] 1.528, 95% confidence interval (CI) 1.041–2.243; p = 0.030), diabetes mellitus type 2 (aOR 1.625, 95% CI 1.130–2.337; p = 0.009), sputum smear positivity grade (3+) (aOR 2.198, 95% CI 1.524–3.168; p < 0.001) and LAM sublineage isolates (aOR 1.023, 95% CI 1.023–2.333; p = 0.039) were associated with the presence of cavitations. Resistance to at least one drug (aOR 25.763, 95% CI 7.096–93.543; p < 0.001) and having isolates other than Haarlem and LAM sublineages (aOR 6.740, 95% CI 1.704–26.661; p = 0.007) were associated with treatment failure. In a second model, multidrug resistance was associated with treatment failure (aOR 31.497, 95% CI 5.119-193.815; p < 0.001). Having more than 6 years of formal education was not associated with treatment failure. Conclusions Knowing M. tuberculosis genetic diversity plays an essential role in disease development and outcomes, and could have important implications for guiding treatment and improving tuberculosis control.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Mycobacterium tuberculosis lineage 4 associated with cavitations and treatment failure

Ordaz-Vázquez

Torres-González

Ferreyra-Reyes

et al. 2022

Preprint

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“…Seven sera were obtained from individuals diagnosed with pulmonary TB from the state of Oaxaca, Mexico, and five samples corresponded to healthy people with no signs of TB. Those samples were obtained from respiratory symptomatic individuals with positive sputum smear microscopy, and TB diagnosis was confirmed by mycobacterial isolation in solid Lowenstein–Jensen media and genotyped through spacer oligonucleotide typing (spoligotyping) . To assess whether Au@PIM hybrids were able to recognize anti-PIM antibodies from TB-infected people, an adapted ELISA was performed using human sera as primary antibodies and antihuman IgG coupled to HRP as secondary ones.…”

Section: Resultsmentioning

confidence: 99%

“…Those samples were obtained from respiratory symptomatic individuals with positive sputum smear microscopy, and TB diagnosis was confirmed by mycobacterial isolation in solid Lowenstein−Jensen media and genotyped through spacer oligonucleotide typing (spoligotyping). 39 To assess whether Au@PIM hybrids were able to recognize anti-PIM antibodies from TB-infected people, an adapted ELISA was performed using human sera as primary antibodies and antihuman IgG coupled to HRP as secondary ones. Although PIMs have not been assessed as antigens in Mexican population, 6 out of 7 TB-positive samples (i.e., 85.7%) showed a positive reactivity to Au@PIM hybrids (Figure 8A).…”

Section: Synthesis and Functionalization Of Aunpsmentioning

confidence: 99%

Mycosome-Coated Gold Nanoparticles for Plasmonic Detection of Tuberculosis-Associated Antibodies

Becerra,

Flores,

Palma-Nicolas

et al. 2024

ACS Appl. Nano Mater.

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Considering that more than 10 million people develop tuberculosis disease (TB) each year and that nearly 3 million of them are never diagnosed, label-free screening methods constitute one of the top public health priorities worldwide. Herein we report the fabrication of a plasmonic system for the detection of TB-associated antibodies. Gold nanoparticles (AuNPs) were synthesized at controlled sizes and modified to display antigen lipid glycans from mycobacteria, the group of organisms comprising the TB causal agent. Facile self-assembly procedures were employed to form mycobacterial phosphatidylinositol mannoside (PIM)containing unilamellar liposomes (mycosomes) and to attach them onto gold surfaces using thiol chemistry. Using enzyme-linked immunoassays with rabbit hyperimmune sera, the constructs were found to specifically capture antilipid antibodies, presenting a dose-dependent response. Also, the functional hybrid nanoparticles showed localized surface plasmon resonance (LSPR) shifts of up to 2 nm upon their interaction with specific antibodies, indicating their suitability for the optical transduction of antigen−antibody reactions. Finally, the applicability of the system for real-world detection of TB-associated antibodies was demonstrated through analysis of 12 samples of human individuals, including 7 cases with pulmonary TB. To the best of our knowledge, this is the first biomimetic nanogold−lipid system for the implementation of lipids as immunosensing reagents for the plasmonic detection and diagnosis of TB.

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Mycobacterium tuberculosis lineage 4 associated with cavitations and treatment failure

et al. 2023

View full text Add to dashboard Cite

Background Mycobacterium tuberculosis genotyping has been crucial to determining the distribution and impact of different families on disease clinical presentation. The aim of the study was to evaluate the associations among sociodemographic and clinical characteristics and M. tuberculosis lineages from patients with pulmonary tuberculosis in Orizaba, Veracruz, Mexico. Methods We analyzed data from 755 patients whose isolates were typified by 24-loci mycobacterial interspersed repetitive unit–variable number of tandem repeats (MIRU–VNTR). The associations among patient characteristics and sublineages found were evaluated using logistic regression analysis. Results Among M. tuberculosis isolates, 730/755 (96.6%) were assigned to eight sublineages of lineage 4 (Euro-American). Alcohol consumption (adjusted odds ratio [aOR] 1.528, 95% confidence interval (CI) 1.041–2.243; p = 0.030), diabetes mellitus type 2 (aOR 1.625, 95% CI 1.130–2.337; p = 0.009), sputum smear positivity grade (3+) (aOR 2.198, 95% CI 1.524–3.168; p < 0.001) and LAM sublineage isolates (aOR 1.023, 95% CI 1.023–2.333; p = 0.039) were associated with the presence of cavitations. Resistance to at least one drug (aOR 25.763, 95% CI 7.096–93.543; p < 0.001) and having isolates other than Haarlem and LAM sublineages (aOR 6.740, 95% CI 1.704–26.661; p = 0.007) were associated with treatment failure. In a second model, multidrug resistance was associated with treatment failure (aOR 31.497, 95% CI 5.119–193.815; p < 0.001). Having more than 6 years of formal education was not associated with treatment failure. Conclusions Knowing M. tuberculosis genetic diversity plays an essential role in disease development and outcomes, and could have important implications for guiding treatment and improving tuberculosis control.

show abstract

RDRio Mycobacterium tuberculosis strains associated with isoniazid resistance in Northern Mexico

Cited by 4 publications

References 11 publications

Mycobacterium tuberculosis lineage 4 associated with cavitations and treatment failure

Mycobacterium tuberculosis lineage 4 associated with cavitations and treatment failure

Mycosome-Coated Gold Nanoparticles for Plasmonic Detection of Tuberculosis-Associated Antibodies

Mycobacterium tuberculosis lineage 4 associated with cavitations and treatment failure

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