rDNA Loci and DAPI Bands Reflect the Phylogenetic Distance between Vicia Species

Ali, Hoda Badry Mohammed; El-Khodary, Soheir; El-Ashry, Zeinab M.; Osman, Samira A.

doi:10.1508/cytologia.74.467

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Molecular cytogenetic approaches like florescence in situ hybridization (FISH) have been applied in different Vicia species [23,24,[49][50][51]. Faba bean (Vicia faba) is the most extensively studied species via in situ hybridization tool to localize the distribution of repetitive sequences and 5S and 45S rDNA on its chromosomes [25,[52][53][54][55][56][57]24].…”

Section: Discussionmentioning

confidence: 99%

Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns

Ali

Osman

2020

Journal of Genetic Engineering and Biotechnology

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Background: Genus Vicia is a member of family Fabaceae and comprises 180 to 210 species. The most important species is faba bean (Vicia faba) which is still one of the most favourable grain legumes over all the world. The genus contains some additional food crops and a number of forage plants and some other weedy strains such as Vicia angustifolia and Vicia cordata. The aim of the present investigation is to elucidate the phylogenetic relationships among four Vicia species, two species (Vicia angustifolia L. ssp. Angustifolia (2n = 12) and Vicia cordata wulfen ex Hoppe (2n = 10)) belong to section Vicia, Vicia dalmatica A. Kern (2n = 12, section Cracca), and Vicia johannis tamamsch (2n = 14, section Faba). Results: Two tools have been applied to identify the genetic relationships among the examined species, double fluorescence in situ hybridization (FISH) has been used to localize the sites of 5S and 45S rDNA, and sodium dodecyl sulfate-poly acrylamide gel electrophoretic (SDS-PAGE) patterns of total seed storage protein fractions. Double FISH experiment has not shown any variation in the loci number, but the positions along the chromosomes were different; both Vicia johannis and Vicia dalmatica exhibited the same interstitial 45S rRNA gene loci, while Vicia angustifolia and Vicia cordata have shown single large stretched 45S rRNA loci almost at the terminal region of the shortest chromosome. It could be concluded from the similarity matrix among the Vicia species as computed according to Jaccard coefficient from the SDS-PAGE, that V. cordata is similar to V. angustifolia and V. dalmatica by a percentage of 73 and 69%, respectively, and the most related species to V. johannis is V. dalmatica (~64%). Conclusion: FISH and SDS-PAGE of the total seed storage proteins together reflected the similar genetic relationship among the studied species as fellows, V. angustifolia is more related to V. cordata then comes V. dalmatica and then V. johannis which is at a distal position from the other species.

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Section: Discussionmentioning

confidence: 99%

Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns

Ali

Osman

2020

Journal of Genetic Engineering and Biotechnology

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“…Molecular cytogenetic techniques like Fluorescence in situ hybridization (FISH) is one of the suitable tools for chromosome identification and analysis which has been applied to many plant genomes (Ali et al 2005(Ali et al , 2009Jiang and Gill 2006;Chiarin and Gauthier 2016;Ribeiro et al 2016;She et al 2017;Tan et al 2017;Setiawan et al 2018;Jiang 2019). FISH using BAC clones depending on the genome mapping was very effective tool to elucidate the comparative cytogenetics map in Lupin Zielinska 2002, 2004;Hajdera et al 2003;Wyrwa et al 2016;Susek et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetical and biochemical studies on some Lupinus species

Ali

Heiba

2021

Bull Natl Res Cent

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Background Lupins are cultivated as human consumption grains and forage legumes. The chromosomes of lupins are too small to be karyotyped by conventional techniques, because they reveal a general lack of distinctive cytological features. In the current study, Fluorescence in situ Hybridization (FISH) was used to locate 5S and 45S ribosomal gene sites on the chromosomes of Lupinus albus ssp albus, Lupinus albus ssp graecus, Lupnus termis (all with 2n = 50), and Lupinus polyphyllus lindl var. polyphyllus (2n = 48), FISH together with seed storage protein electrophoretic patterns were used to find out the relationship among these species. Results The double-target FISH on the chromosomes of the studied species with rDNA probes revealed that the two types of rRNA genes are located on different chromosomes. The detected loci of rRNA genes partially reflected the taxonomical similarity among the two Lupinus albus subspecies and L. termis. Lupinus polyphyllus lindl var. polyphyllus was exception by having unique large chromosome mostly is covered by one signal of 45S rDNA, whereas its homologous chromosome seems to be normal-sized and have the other 45S rDNA locus. The similarity matrix among the Lupinus species as computed according to Jaccardʼs Coefficient from the SDS-PAGE, showed that L. albus ssp. Albus and L. albus ssp. Graecus are the most similar species (~ 97%), and then comes L. termis, and L. polyphyllus lindl var. polyphylus has been placed in separate clade and still the most related species to it among the studied species is L. termis (~ 70%). Conclusion It could be postulated from FISH and seed storage protein electrophoretic patterns that the relationships among the studied species is as follows, Lupinus albus ssp albus, is the most related species to Lupinus albus ssp graecus then comes Lupnus termis and Lupinus polyphyllus lindl var. polyphyllus at a distal position.

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Chromosomal characterization based on repetitive DNA distribution in a tetraploid cytotype of Chrysanthemum zawadskii

Cuyacot

Lim

Kim

et al. 2017

Hortic. Environ. Biotechnol.

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rDNA Loci and DAPI Bands Reflect the Phylogenetic Distance between Vicia Species

Cited by 3 publications

References 25 publications

Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns

Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns

Molecular cytogenetical and biochemical studies on some Lupinus species

Chromosomal characterization based on repetitive DNA distribution in a tetraploid cytotype of Chrysanthemum zawadskii

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