Rdh54/Tid1 Inhibits Rad51-Rad54-Mediated D-loop Formation and Limits D-loop Length

Shah, Shanaya Shital; Hartono, Stella R.; Som, Vanessa; Wright, William D.; Chédin, Frédéric; Heyer, Wolf‐Dietrich

doi:10.1101/2020.05.20.106641

Cited by 3 publications

(2 citation statements)

References 64 publications

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“…These factors stimulate Rad51 in the recombination stage and can inhibit the binding of Rad51 to dsDNA to expose 3 0 -OH terminus for DNA synthesis. 96,98,99 In the negative regulation of this pathway, some factors, such as and Mhf2 proteins suggested to help Mph1 activity. 101,102 In addition, F-box DNA helicase 1 (FBH1) has anti-recombinase activity, which facilitates the removal of Rad51.…”

Section: Homologous Recombination Pathwaymentioning

confidence: 99%

Melatonin: A smart molecule in the DNA repair system

Mir

Aliarab

Goodarzi

et al. 2021

Cell Biochemistry & Function

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Award Number: 112351 DNA repair is an important pathway for the protection of DNA molecules from destruction. DNA damage can be produced by oxidative reactive nitrogen or oxygen species, irritation, alkylating agents, depurination and depyrimidination; in this regard, DNA repair pathways can neutralize the negative effects of these factors. Melatonin is a hormone secreted from the pineal gland with an antioxidant effect by binding to oxidative factors. In addition, the effect of melatonin on DNA repair pathways has been proven by the literature. DNA repair is carried out by several mechanisms, of which homologous recombination repair (HRR) and non-homologous end-joining (NHEJ) are of great importance. Because of the importance of DNA repair in DNA integrity and the anticancer effect of this pathway, we presented the effect of melatonin on DNA repair factors regarding previous studies conducted in this area.

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Section: Homologous Recombination Pathwaymentioning

confidence: 99%

Melatonin: A smart molecule in the DNA repair system

Mir

Aliarab

Goodarzi

et al. 2021

Cell Biochemistry & Function

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“…It is also possible to extend the assay to map other DNA intermediates/structures that include some regions of single-stranded DNA. Using the D-loop Mapping Assay we could show that Rdh54/Tid1 antagonizes Rad54 in D-loop formation and restricts D-loops length(Shah et al 2020).…”

mentioning

confidence: 99%

Bisulfite treatment and single-molecule real-time sequencing reveal D-loop length, position, and distribution

Shah

Hartono

Chédin

et al. 2020

eLife

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Displacement loops (D-loops) are signature intermediates formed during homologous recombination. Numerous factors regulate D-loop formation and disruption, thereby influencing crucial aspects of DNA repair, including donor choice and the possibility of crossover outcome. While D-loop detection methods exist, it is currently unfeasible to assess the relationship between D-loop editors and D-loop characteristics such as length and position. Here, we developed a novel in vitro assay to characterize the length and position of individual D-loops with near base-pair resolution and deep coverage, while also revealing their distribution in a population. Non-denaturing bisulfite treatment modifies the cytosines on the displaced strand of the D-loop to uracil, leaving a permanent signature for the displaced strand. Subsequent single-molecule real-time sequencing uncovers the cytosine conversion patch as a D-loop footprint. The D-loop Mapping Assay is widely applicable with different substrates and donor types and can be used to study factors that influence D-loop properties.

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Non-denaturing bisulfite treatment and single-molecule real-time sequencing reveals d-loop footprints, their length, position and distribution

Shah¹,

Hartono²,

Chédin³

et al. 2020

Preprint

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Displacement loops (D-loops) are signature intermediates formed during homologous recombination. Numerous factors regulate D-loop formation and disruption, thereby influencing crucial aspects of DNA repair, including donor choice and the possibility of a crossover outcome. While D-loop detection methods exist, it is currently unfeasible to assess the relationship between D-loop editors and D-loop characteristics such as length and position. Here, we developed a novel in vitro assay to characterize the length and position of individual D-loop with base-pair resolution and deep coverage, while also revealing their distribution in a population. Non-denaturing bisulfite treatment modifies the cytosines on the displaced strand of the D-loop to uracil, leaving a permanent signature for the displaced strand. Subsequent single-molecule real-time sequencing uncovers the cytosine conversion patch as a D-loop footprint, revealing D-loop characteristics at unprecedented resolution. The D-loop Mapping Assay is widely applicable with different substrates and donor types and can be used to study factors that influence D-loop properties.

show abstract

Rdh54/Tid1 Inhibits Rad51-Rad54-Mediated D-loop Formation and Limits D-loop Length

Cited by 3 publications

References 64 publications

Melatonin: A smart molecule in the DNA repair system

Melatonin: A smart molecule in the DNA repair system

Bisulfite treatment and single-molecule real-time sequencing reveal D-loop length, position, and distribution

Non-denaturing bisulfite treatment and single-molecule real-time sequencing reveals d-loop footprints, their length, position and distribution

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