RBM15 Promates the Proliferation, Migration and Invasion of Pancreatic Cancer Cell Lines

Dong, Hui; Zhang, Haidong; Mao, Xinyu; Liu, Shiwei; Xu, Wenjing; Zhang, Yewei

doi:10.3390/cancers15041084

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…It has been reported that RBM15 is capable of facilitating cell proliferation in some cancers ( 16 , 17 , 35 ), but this has not been reported in OC. For example, RBM15 enhances the proliferation, migration and invasion of clear cell renal cell carcinoma ( 36 ).…”

Section: Discussionmentioning

confidence: 93%

RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Yuan

Guan

et al. 2023

Int J Oncol

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Ovarian cancer (OC) lacks effective biomarkers for diagnosis at an early stage and often develops chemoresistance after the initial treatment at an advanced stage. RNA-binding motif protein 15 (RBM15) is an RNA m 6 A methylation mediator that serves an oncogenic role in some cancers. However, the function and molecular mechanisms of RBM15 in ovarian tumorigenesis and chemoresistance remain to be elucidated. The present study identified the overexpression of RBM15 in OC tissues and paclitaxel (PTX)-resistant cells using reverse transcription-quantitative (q)PCR, western blotting and immunohistochemistry. Clinical data analyses showed that high expression of RBM15 was associated with poor prognosis in patients with OC. Overexpression of RBM15 led to an increase in cell viability and colony formation and a decrease in cell sensitivity to PTX and apoptosis, whereas the knockdown of RBM15 resulted in the inhibition of cell viability and colony formation in vitro and tumor formation in vivo and increased cell apoptosis and sensitivity to PTX in a time-and dose-dependent manner. Furthermore, RBM15 knockdown reduced the spheroid formation of PTX-resistant OC cells. Silencing of RBM15 decreased multidrug resistance 1 (MDR1) mRNA m 6 A methylation detected by the methylated RNA immunoprecipitation-qPCR assay and downregulated the expression of a chemo-drug efflux pump MDR1 at the mRNA and protein levels. Finally, RBM15 expression was suppressed by the activation of the TGF-β signaling pathway. Thus, the findings revealed a TGF-β/RBM15/MDR1 regulatory mechanism. Targeting RBM15 may provide a novel therapeutic strategy for the treatment of PTX-resistant OC.

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Section: Discussionmentioning

confidence: 93%

RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Yuan

Guan

et al. 2023

Int J Oncol

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“…Plexins are known for their key role in tumor CCC, tumor growth, migration, and metastasis. Semaphorins are the main ligands of the Plexin receptors; however, some plexins can also form complexes with other tyrosine-kinase receptors, such as the hepatocyte growth factor receptor (HGFR) encoded by MET 65 and RON encoded by MST1R 66 . NEST reported a PLXNB2 - MET / MSTR1 CCC pattern that is favored in Classical over Basal areas of the tumor.…”

Section: Resultsmentioning

confidence: 99%

NEST: Spatially-mapped cell-cell communication patterns using a deep learning-based attention mechanism

Zohora,

Flores-Figueroa,

et al. 2024

Preprint

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Dysregulation of communication between cells mediates complex diseases such as cancer and diabetes. However, detecting cell-cell communication (CCC) at scale remains one of the greatest challenges in transcriptomics. While gene expression measured with single-cell RNA sequencing and spatial transcriptomics reinvigorated computational approaches to detecting CCC, most existing methods exhibit high false positive rates, do not integrate spatial proximity of ligand-receptor interactions, and cannot detect CCC between individual cells. We overcome these challenges by presentingNEST (NEural network on Spatial Transcriptomics), which uses a graph attention network paired with an unsupervised contrastive learning approach to decipher patterns of communication while retaining the strength of each signal. We introduce new synthetic benchmarking experiments which demonstrate how NEST outperforms existing tools and detects biologically-relevant CCC along with directionality and confidence across spot- and cell-based technologies measuring several different tissues and diseases. In our applications, NEST identifies T-cell homing signals in human lymph nodes, aggressive cancer CCC in lung adenocarcinoma, and discovers new patterns of communication that act as relay networks in pancreatic cancer. Beyond two-dimensional data, we also highlight NEST’s ability to detect CCC in three-dimensional spatial transcriptomic data.

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“…The gene synthesizes the RBM15 protein, which is analogous to RBM15B [ 24 , 102 ]. RBM15 and RBM15B lack enzymatic activity; however, they can physically associate with METTL3 and WTAP, thereby facilitating their localization to specific RNA sites for m6A modification [ 103 , 104 ].…”

Section: M6a Regulatorsmentioning

confidence: 99%

Role of m6A modification in regulating the PI3K/AKT signaling pathway in cancer

Liu,

Gu,

Guan

et al. 2023

J Transl Med

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The phosphoinositide 3-kinase (PI3K)/AKT signaling pathway plays a crucial role in the pathogenesis of cancer. The dysregulation of this pathway has been linked to the development and initiation of various types of cancer. Recently, epigenetic modifications, particularly N6-methyladenosine (m6A), have been recognized as essential contributors to mRNA-related biological processes and translation. The abnormal expression of m6A modification enzymes has been associated with oncogenesis, tumor progression, and drug resistance. Here, we review the role of m6A modification in regulating the PI3K/AKT pathway in cancer and its implications in the development of novel strategies for cancer treatment.

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RBM15 Promates the Proliferation, Migration and Invasion of Pancreatic Cancer Cell Lines

Cited by 8 publications

References 26 publications

RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

NEST: Spatially-mapped cell-cell communication patterns using a deep learning-based attention mechanism

Role of m6A modification in regulating the PI3K/AKT signaling pathway in cancer

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