Rbbp7 Is Required for Uterine Stromal Decidualization in Mice1

Abstract

Uterine stromal cells undergo extensive proliferation and differentiation during postimplantation development, a process known as decidualization. While a range of signaling molecules have been demonstrated to play essential roles in this event, its potential epigenetic regulatory mechanisms remain largely unknown. Retinoblastoma binding protein 7 (Rbbp7) is a protein reported as a core component of many histone modification and chromatin remodeling complexes. In the present study, our in situ hybridization and immunochemistry analysis first reveals a spatiotemporal expression of Rbbp7 in the uterus during the peri-implantation period. Observations of remarkable induction of Rbbp7 expression in uterine stromal cells in response to progesterone-nuclear receptor PR signaling point to its potential physiological significance during postimplantation uterine development. Employing a stealth RNA knockdown approach, combined with primary murine uterine stromal cell culture and an in vitro-induced decidualization model, we further demonstrate that Rbbp7 silencing compromises stromal cell decidualization via attenuating histone H4 acetylation and cyclin D3 expression. The results collectively suggest that Rbbp7 is a potentially functional player regulating normal histone acetylation modification and cyclin D3 expression in stromal cells during postimplantation decidual development.

The E3 ubiquitin ligase RNF 114 and TAB 1 degradation are required for maternal‐to‐zygotic transition

“…HEK293T cells were co‐transfected with plasmids expressing RGS‐His‐tagged WT or mutant Rnf114, EGFP, and each substrate including Flag‐Cd74, Flag‐Tab1, Flag‐Optn, Flag‐Tnip1, Flag‐Appl1, Flag‐Psat1, Flag‐Ralgps, Flag‐Dcn1, Flag‐Tom1 l2, or Flag‐Rab3il1 with Lipofectamine 2000 (Invitrogen). Cells were lysed in lysis buffer containing protease inhibitors and subjected to Western blotting with antibodies against FLAG or RGS‐His as reported previously (detail information of antibodies is given in Table EV2).…”

SPOP Regulates Endometrial Stromal Cell Decidualization in Mice

“…The tissues were fixed in 4% paraformaldehyde, dehydrated via graded ethanol solutions, and embedded in paraffin. 32 The procedure for the IHC analysis was described previously. 33 The tissue sections were dewaxed in xylene and rehydrated in descending concentrations of ethanol, followed by antigen retrieval and cooling to room temperature.…”

Ubiquitin‐specific protease 7 (USP7) is essential for endometrial stromal cell decidualization in mice

“…Normal decidua development is essential for successful pregnancy as it functions to nourish the embryo by providing a barrier to prevent uncontrolled trophoblast invasion before the formation of a mature placenta (Das et al., ). Hence, it is important to obtain a deeper understanding of the regulatory molecules and mechanisms in early pregnancy (Dey et al., ; He et al., ). Given the observation from previous reports that USP7 knockout mice had a dramatic reduction in proliferation and embryonic development between days E6.5 and E7.5 post coitum (He et al., ; Scherle et al., ), we designed experiments to clarify its function in uterine stroma decidualization, a key event in pregnancy.…”