RB137 and RB138 antibodies recognize human cathelicidin LL37 by ELISA

Palazzo, Raffaella; Pietraforte, Immacolata; Chizzolini, Carlo; Lande, Roberto; Frasca, Loredana

doi:10.24450/journals/abrep.2020.e188

Antib. Rep.

2020

DOI: 10.24450/journals/abrep.2020.e188

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RB137 and RB138 antibodies recognize human cathelicidin LL37 by ELISA

Raffaella Palazzo¹,

Immacolata Pietraforte²,

Carlo Chizzolini³

et al.

Abstract: LL37 is a cationic antimicrobial peptide (AMP), which can undergo post-translational modifications (PTM), such as citrullination and carbamylation. We demonstrate here that recombinant antibodies RB137 and RB138 are specific for native LL37 and do not recognize modified LL37 (citrullinated and carbamylated). They thus represent tools to assess the presence of native, unmodified LL37 in body fluids by ELISA.

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RB137 recognizes LL37 in neutrophil-extracellular trap-like (NET) structures in systemic lupus erythematosus and rheumatoid arthritis inflamed tissues by immunofluorescence in histological sections

Lande¹,

Alivernini²,

Tolusso³

et al. 2020

Antib. Rep.

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Besides being a natural antibiotic, the human cathelicidin LL37, produced by epithelial cells and neutrophils, is an important immune-modulator. LL37 alone, or in complex with DNA, can activate inflammatory pathways in psoriasis, Systemic Lupus Erythematosus (SLE) and Rheumatoid Arthritis (RA). In this work, we describe the capacity of the recombinant monoclonal antibody RB137, previously shown to specifically recognize LL37 in its native form by ELISA, to detect LL37 by immunofluorescence in human tissues derived from SLE and RA patients. In these tissues, LL37 decorates DNA filaments resembling neutrophil-extracellular-trap (NET) structures. This antibody represents a valuable tool to detect the presence, the native state and the exact localization of LL37 in human tissues in health and disease.

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RB137 recognizes LL37 in neutrophil-extracellular trap-like (NET) structures in systemic lupus erythematosus and rheumatoid arthritis inflamed tissues by immunofluorescence in histological sections

Lande¹,

Alivernini²,

Tolusso³

et al. 2020

Antib. Rep.

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show abstract

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RB137 and RB138 antibodies recognize human cathelicidin LL37 by ELISA

Cited by 1 publication

References 5 publications

RB137 recognizes LL37 in neutrophil-extracellular trap-like (NET) structures in systemic lupus erythematosus and rheumatoid arthritis inflamed tissues by immunofluorescence in histological sections

RB137 recognizes LL37 in neutrophil-extracellular trap-like (NET) structures in systemic lupus erythematosus and rheumatoid arthritis inflamed tissues by immunofluorescence in histological sections

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