Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wound healing in genetically diabetic mice*1

Galeano, Massimiliano; Torre, Valerio; Deodato, Barbara; Campo, Giuliana Del; Colonna, M.; Sturiale, Alessio; Squadrito, F.; Cavallari, Vittorio; Cucinotta, Daniela; Buemi, Michele

doi:10.1016/s0039-6060(01)79415-9

Cited by 10 publications

(15 citation statements)

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“…The following parameters were evaluated and scored: re-epithelialization, dermal matrix deposition and regeneration, granulation tissue formation and remodelling. The histological specimens were evaluated according to the score reported in the literature concerning wound healing in experimental models [20,22–24]. …”

Section: Methodsmentioning

confidence: 99%

Relaxin improves multiple markers of wound healing and ameliorates the disturbed healing pattern of genetically diabetic mice

et al. 2013

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Diabetic mice are characterized by a disrupted expression pattern of VEGF (vascular endothelial growth factor), and impaired vasculogenesis during healing. Experimental evidence suggests that RLX (relaxin) can improve several parameters associated with wound healing. Therefore we investigated the effects of porcine-derived RLX in diabetes-related wound-healing defects in genetically diabetic mice. An incisional wound model was produced on the back of female diabetic C57BL/KsJ-m+/+Leptdb (db+/db+) mice and their normal littermates (db+/+m). Animals were treated daily with porcine RLX (25 μg/mouse per day, subcutaneously) or its vehicle. Mice were killed on 3, 6 and 12 days after skin injury for measurements of VEGF mRNA and protein synthesis, SDF-1α (stromal cell-derived factor-1α) mRNA and eNOS (endothelial NO synthase) expression. Furthermore, we evaluated wound-breaking strength, histological changes, angiogenesis and vasculogenesis at day 12. Diabetic animals showed a reduced expression of VEGF, eNOS and SDF-1α compared with non-diabetic animals. At day 6, RLX administration resulted in an increase in VEGF mRNA expression and protein wound content, in eNOS expression and in SDF-1α mRNA. Furthermore, the histological evaluation indicated that RLX improved the impaired wound healing, enhanced the staining of MMP-11 (matrix metalloproteinase-11) and increased wound-breaking strength at day 12 in diabetic mice. Immunohistochemistry showed that RLX in diabetic animals augmented new vessel formation by stimulating both angiogenesis and vasculogenesis. RLX significantly reduced the time to complete skin normalization and this effect was abrogated by a concomitant treatment with antibodies against VEGF and CXCR4 (CXC chemokine receptor 4), the SDF-1α receptor. These data strongly suggest that RLX may have a potential application in diabetes-related wound disorders.

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Section: Methodsmentioning

confidence: 99%

Relaxin improves multiple markers of wound healing and ameliorates the disturbed healing pattern of genetically diabetic mice

et al. 2013

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“…The fine balance of these nutrients has to be taken into account, though. For example, with vitamin E, an important lipophilic antioxidant, conflicting studies have both shown reduced tensile strength and collagen content of experimental wounds [13, 14] and, conversely, increased wound strength and collagen [15]. As with all aspects of wound repair, a fine balance is necessary to ultimately achieve proper healing.…”

Section: Physiologic Wound Healingmentioning

confidence: 99%

Chronic Wound Healing: A Review of Current Management and Treatments

2017

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Wound healing is a complex, highly regulated process that is critical in maintaining the barrier function of skin. With numerous disease processes, the cascade of events involved in wound healing can be affected, resulting in chronic, non-healing wounds that subject the patient to significant discomfort and distress while draining the medical system of an enormous amount of resources. The healing of a superficial wound requires many factors to work in concert, and wound dressings and treatments have evolved considerably to address possible barriers to wound healing, ranging from infection to hypoxia. Even optimally, wound tissue never reaches its pre-injured strength and multiple aberrant healing states can result in chronic non-healing wounds. This article will review wound healing physiology and discuss current approaches for treating a wound.

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“…The negative effects of oxidative stress can be restrained by antioxidant enzyme systems and dietary antioxidants such as carotenoids 5. The carotenoid structure has a common chemical feature containing a long-conjugated double-bond polyene chain, which has an ability to quench or scavenge ROS 6. Several kinds of antioxidants are proposed to regulate the oxidation–reduction balance, and studies of the effects of various antioxidants on wound healing have reported accelerated healing outcomes 7,8…”

Section: Introductionmentioning

confidence: 99%

Effect of astaxanthin on cutaneous wound healing

Meephansan¹,

Rungjang²,

Yingmema³

et al. 2017

CCID

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Wound healing consists of a complex series of convoluted processes which involve renewal of the skin after injury. ROS are involved in all phases of wound healing. A balance between oxidative and antioxidative forces is necessary for a favorable healing outcome. Astaxanthin, a member of the xanthophyll group, is considered a powerful antioxidant. In this study, we investigated the effect of topical astaxanthin on cutaneous wound healing. Full-thickness dermal wounds were created in 36 healthy female mice, which were divided into a control group and a group receiving 78.9 µM topical astaxanthin treatment twice daily for 15 days. Astaxanthin-treated wounds showed noticeable contraction by day 3 of treatment and complete wound closure by day 9, whereas the wounds of control mice revealed only partial epithelialization and still carried scabs. Wound healing biological markers including Col1A1 and bFGF were significantly increased in the astaxanthin-treated group since day 1. Interestingly, the oxidative stress marker iNOS showed a significantly lower expression in the study. The results indicate that astaxanthin is an effective compound for accelerating wound healing.

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Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wound healing in genetically diabetic mice*1

Abstract: The current study provides evidence that raxofelast restores wound healing to nearly normal levels in experimental diabetes-impaired wounds and suggests that an increased lipid peroxidation in diabetic mice may have a role in determining a defect of wound repair.

Cited by 10 publications

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Relaxin improves multiple markers of wound healing and ameliorates the disturbed healing pattern of genetically diabetic mice

Relaxin improves multiple markers of wound healing and ameliorates the disturbed healing pattern of genetically diabetic mice

Chronic Wound Healing: A Review of Current Management and Treatments

Effect of astaxanthin on cutaneous wound healing

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