Ratlarda Toluenle İndüklenen Oksidatif Hasara Karşı p-Kumarik Asitin Antioksidan Etkisi

Abstract

This research aimed to investigate the protective effect of p-CA, a derivative of phenolic acid, against toluene-induced oxidative damage. A total of 32 Sprague-Dawley male rats, 8 in each group, were used. A total of 4 groups were formed as control, toluene, p-CA and toluene+p-CA. Animals in the control group, toluene group and p-CA group were given 0.9 % NaCl, 0.9 mg kg-1 b.w toluene and 100 mg kg -1 b.w p-CA orally for 21 days, respectively. The animals in toluene+p-CA group were received p-CA for 3 days and from day 4, toluene and p-CA were applied together daily until day 25. On the 25th day, the study was terminated, and blood samples were collected. Catalase (CAT) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) and glutathione (GSH) levels in the erythrocytes and superoxide dismutase (SOD) activity in plasma samples were determined. In this study, CAT and SOD activities and MDA level significantly (p <0.05) increased, GSH level and GSH-Px activity significantly (p <0.05) decreased in the blood samples of toluene group compared to the control group. In toluene+p-CA group, significant (p <0.05) increases in GSH level and GSH-Px activity and significant (p <0.05) decreases in MDA level and SOD and CAT activities were detected compared to the toluene group. It has been determined that toluene caused oxidative stress and lipid peroxidation in blood tissue and against this oxidative damage, p-CA had a protective effect.

Efektivitas Ekstrak Etanol Tauge (Vigna Radiata) Dalam Meningkatkan Kadar Glutation Peroksidase Pada Tikus Putih (Rattus Norvegicus) Yang Diinduksi Toluena

“…Penggunaan toluena yang melebihi ambang batas atau dalam jangka waktu yang lama dapat menyebabkan penyakit akibat kerja (Cindy et al, 2022). Meskipun tidak digolongkan ke dalam senyawa karsinogenik, paparan toluena secara terus-menerus dapat memberikan dampak negatif, yaitu mengakibatkan gangguan kesehatan seperti iritasi pada mata dan tenggorokan, asma, gangguan sistem saraf pusat, hingga gangguan reproduksi (Erol et al, 2021;Prayogi et al, 2020). Penelitian Anyanwu et al membuktikan bahwa pekerja dengan mobilitas yang tinggi memiliki risiko yang tinggi mengalami hepatotoksisitas dan hepatoselular karsinogenesis (Nsonwu-Anyanwu, 2021).…”

“…Toluena yang masuk ke dalam tubuh dapat dimetabolisme lebih lanjut menjadi benzil alkohol dengan bantuan CYP2E1. Pada penelitian Erol et al induksi toluena pada tikus meningkatkan kadar malondialdehid (MDA) yang merupakan salah satu produk dari pembentukan ROS (Erol et al, 2021).…”

“…Jumlah ROS yang berlebih tanpa diimbangi dengan peningkatan antioksidan dapat menyebabkan kerusakan sel. Antioksidan dapat berupa antioksidan enzimatik, seperti SOD, katalase, dan glutation peroksidase (GPX) dan antioksidan nonenzimatik, seperti vitamin C dan vitamin E (Erol et al, 2021). Keadaan dimana terjadi ketidakseimbangan prooksidan dan antioksidan disebut dengan stres oksidatif (Montes, Yee-Rios, & Páez-Martínez, 2018).…”