Rationally designed short cationic α-helical peptides with selective anticancer activity

Hadianamrei, Roja; Tomeh, Mhd Anas; Brown, Stephen; Wang, Jiqian; Zhao, Xiubo

doi:10.1016/j.jcis.2021.08.200

Cited by 42 publications

(34 citation statements)

References 92 publications

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“…This cell death induced in the spheroids was significantly higher than that induced by other anticancer peptide-based systems. 26 These observations were finally substantiated cell viability data observed with MTT assay and live/dead assay. These findings highlight the potential of Pep5-TPPN as an effective therapeutic approach for breast cancer treatment.…”

Section: Resultssupporting

confidence: 55%

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Multi-Layered PLGA-PEI Nanoparticles Functionalized with TKD Peptide for Targeted Delivery of Pep5 to Breast Tumor Cells and Spheroids

Mohan¹,

Minsa²,

Kumar³

et al. 2022

IJN

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Purpose Peptide-based therapy is a promising strategy for cancer treatment because of its low drug resistance. However, the major challenge is their inability to target cancer cells specifically. So, a targeted nano-delivery system that could deliver therapeutic peptides selectively to cancer cells to stimulate their action is highly desirable. This study aims to deliver the antitumor peptide, Pep5, to breast tumor cells selectively using a targeting peptide functionalised multi-layered PLGA-PEI nanoparticles. Methods In this study, Pep5 entrapped PLGA-PEI (Pep5-PPN) dual layered nanoparticles were developed. These nanoparticles were decorated with TKD (Pep5-TPPN) on their surface for site-specific delivery of Pep5 to breast tumor cells. The particles were then characterized using various instrumental analyses. In vitro cytotoxicity of the particles was evaluated in estrogen receptor positive (ER +ve ) and triple negative breast cancer (TNBC) cells. An ex vivo tumor spheroid model was used to analyze the antitumor activity of the particles. Results Uniformly round Pep5-TPPN particles were synthesized with an average diameter of 420.8 ± 14.72 nm. The conjugation of PEI over Pep5-PLGA nanoparticles shifted the zeta potential from −11.6 ± 2.16 mV to +20.01 ± 2.97 mV. In vitro cytotoxicity analysis proved that TKD conjugation to nanoparticles enhanced the antitumor activity of Pep5 in tested breast cancer cells. Pep5-TPPN induced cytoskeletal damage and apoptosis in the tested cells, which showed that the mechanism of action of Pep5 is conserved but potentiated. Active targeting of Pep5 suppressed the tumor growth in ex vivo spheroid models. Conclusion A multi-layered nanoparticle functionalized with dual peptide was fabricated for active tumor targeting, which stimulated Pep5 activity to reduce the tumor growth in vitro and ex vivo.

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Section: Resultssupporting

confidence: 55%

“…Other anticancer peptides were also reported to change the microfilament organisation. 26 The loss of integrity of actin filament could be attributed to the binding of Pep5 to Plectin, which hampers their ability to organize the cytoskeleton by interfering with the protein–protein interaction. 6 , 45 …”

Section: Resultsmentioning

confidence: 99%

Multi-Layered PLGA-PEI Nanoparticles Functionalized with TKD Peptide for Targeted Delivery of Pep5 to Breast Tumor Cells and Spheroids

Mohan¹,

Minsa²,

Kumar³

et al. 2022

IJN

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“…Biomaterials Science reported in a previous study on cationic amphiphilic peptides with higher selectivity for negatively charged cancer cells over normal mammalian cells. 57 Fig. 4B and D showed an excellent correlation between the increase of the surface pressure (ΔΠ) and the peptide helicities in DPPC and DPPG, indicating that the peptides with higher helicities have better antimicrobial effects.…”

Section: Papermentioning

confidence: 80%

“…This has also been evidenced in our recent study on another group of cationic amphiphilic peptides with selective anticancer activity. 57…”

Section: Resultsmentioning

confidence: 99%

Highly selective performance of rationally designed antimicrobial peptides based on ponericin-W1

Wang

You

et al. 2022

Biomater. Sci.

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“…1(I)). 39 Dynamic oscillatory rheological measurements were performed to investigate the viscoelastic properties of both KP and KPFe hydrogels. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of injectable hydrogels from an anticancer peptide for local therapeutic delivery and synergistic photothermal–chemotherapy

Liu

Tian

et al. 2022

J. Mater. Chem. B

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Rationally designed short cationic α-helical peptides with selective anticancer activity

Cited by 42 publications

References 92 publications

Multi-Layered PLGA-PEI Nanoparticles Functionalized with TKD Peptide for Targeted Delivery of Pep5 to Breast Tumor Cells and Spheroids

Multi-Layered PLGA-PEI Nanoparticles Functionalized with TKD Peptide for Targeted Delivery of Pep5 to Breast Tumor Cells and Spheroids

Highly selective performance of rationally designed antimicrobial peptides based on ponericin-W1

Fabrication of injectable hydrogels from an anticancer peptide for local therapeutic delivery and synergistic photothermal–chemotherapy

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