Rationally Designed Peptidyl Virus‐Like Particles Enable Targeted Delivery of Genetic Cargo

Kong, Jia; Wang, Yuefei; Zhang, Jiaxing; Qi, Wei; Su, Rongxin; He, Zhimin

doi:10.1002/ange.201805868

Cited by 10 publications

(7 citation statements)

References 40 publications

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“…Another work from the same group demonstrated that the formed nanoribbons achieved high gene-transfection efficacy in HEK293 cells 51. Although the detailed mechanism of this observation remain to be elucidated, this work may lead to a facile approach to fabricate viral-mimetic nanostructure 52 with certain stability in vitro .…”

Section: Virus-like Peptide Nanostructures For Gene Deliverymentioning

confidence: 82%

Supramolecular Assemblies of Peptides or Nucleopeptides for Gene Delivery

Wang¹,

Feng²,

Xu³

2019

Theranostics

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Using non-covalent interactions between nucleic acids (DNA, siRNA, miRNA, and mRNA) with peptides or nucleopeptides is a promising strategy to construct supramolecular assemblies for gene delivery and therapy. Comparing to conventional strategies for gene delivery, the assemblies of peptides or nucleopeptides provide several unique advantages: i) reversible interactions between the assemblies and the nucleic acids; ii) minimal immunogenicity; iii) biocompatibility. This field has advanced considerably in recent years so that it is worth summarizing the recent progresses and future challenges. In this review, we introduce the development of assemblies of peptides or nucleopeptides for applications in gene delivery and related fields. After introducing the promises of gene therapy and the current strategies for the delivery, we discuss the unique advantage of using peptide assemblies for gene delivery. Then we describe several representative strategies for gene delivery by the assemblies of peptides or nucleopeptides. Finally, we discuss the key factors for designing such assemblies for gene delivery, and speculate future directions and challenges in the field, particularly the rational design and the spatiotemporally controlled release in live cells.

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Section: Virus-like Peptide Nanostructures For Gene Deliverymentioning

confidence: 82%

Supramolecular Assemblies of Peptides or Nucleopeptides for Gene Delivery

Wang¹,

Feng²,

Xu³

2019

Theranostics

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“…These NPs possess a strong potential to deliver the CRISPR/Cas9 system within specific cells [98]. Out of these NPs, black phosphorus NPs, calcium carbonate NPs, and notably AuNPs have attracted the attention to develop more advanced carrier structures (Table 2) [11, 12, 53, 99–124].…”

Section: Delivery Strategies Of the Crispr/cas9 Complex Within Cancer...mentioning

confidence: 99%

Current updates of CRISPR/Cas9‐mediated genome editing and targeting within tumor cells: an innovative strategy of cancer management

Allemailem

Alsahli

Almatroudi

et al. 2022

Cancer Communications

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Clustered regularly interspaced short palindromic repeats-associated protein (CRISPR/Cas9), an adaptive microbial immune system, has been exploited as a robust, accurate, efficient and programmable method for genome targeting and editing. This innovative and revolutionary technique can play a significant role in animal modeling, in vivo genome therapy, engineered cell therapy, cancer diagnosis and treatment. The CRISPR/Cas9 endonuclease system targets a specific genomic locus by single guide RNA (sgRNA), forming a heteroduplex with target DNA. The Streptococcus pyogenes Cas9/sgRNA:DNA complex reveals a bilobed architecture with target recognition and nuclease lobes. CRISPR/Cas9 assembly can be hijacked, and its nanoformulation can be engineered as a delivery system for different clinical utilizations. However, the efficient and safe delivery of the CRISPR/Cas9 system to target tissues and cancer cells is very challenging, limiting its clinical utilization. Viral delivery strategies of this system may have many advantages, but disadvantages such as immune system stimulation, tumor promotion risk and small insertion size outweigh these advantages. Thus, there is a desperate need to develop an efficient non-viral physical delivery system based on simple nanoformulations. The delivery strategies of CRISPR/Cas9 by a nanoparticle-based system

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“…In recent years, peptide-based materials have received increasing attention in the biomedical field because of their structural versatility and good biocompatibility. [114][115][116] Functionalizable peptide materials can be the basis for constructing artificial enzymes. Fry et al prepared an artificial peroxidase with tunable catalytic activity by modulating the peptide sequence and the structure of the peptide-based assemblies.…”

Section: Peptide-based Materials@mipsmentioning

confidence: 99%