Rational Modification of Ligand‐Binding Preference of Avidin by Circular Permutation and Mutagenesis

Abstract

An error has been found in the interpretation of the mass spectroscopy data in this paper. On p. 1125, the section under the heading "Mass spectrometry" should read: Electrospray ionization Fourier transform ion cyclotron resonance (ESI FT-ICR) mass spectrometry was used to confirm the amino acid sequences and proper folding of the proteins (mass spectra not presented). For some unknown reason, dcAvd2 was the only protein that could not be identified by this method. A possible reason could be the fragmentation of the protein, which can also be seen in the SDS-PAGE analysis (Figure 1 A). The most abundant isotopic masses were determined to be 14 685.52 AE 0.