Rational medium design for Bordetella pertussis: basic metabolism

Identification of a Novel Lipopolysaccharide Core Biosynthesis Gene Cluster in Bordetella pertussis , and Influence of Core Structure and Lipid A Glucosamine Substitution on Endotoxic Activity

“…When appropriate, bacteria were grown in the presence of 100 g/ml ampicillin, 50 g/ml kanamycin, or 10 g/ml gentamicin for plasmid maintenance or strain selection. B. pertussis was grown in synthetic THIJS medium (30) or on Bordet-Gengou agar supplemented with 15% defibrinated sheep blood (Tritium) at 35°C.…”

Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica

“…The metabolism of B. pertussis has been studied in some detail; it can grow in culture on glutamate and cysteine 14 . In laboratory media, cysteine is present at low levels and probably serves as a source of sulfur rather than carbon, suggesting that glutamate is the main carbon source for cultured B. pertussis.…”

“…It has been reported that the tricarboxylic acid cycle in the bordetellae is non-functional, based on the observation that in these bacteria, acetyl-coenzyme A and oxaloacetate did not give rise to α-ketoglutarate 14 . Genes encoding all the main tricarboxylic acid cycle enzymes are present in all three species, however.…”

Immunodominance in Mouse and Human CD4+T-Cell Responses Specific for theBordetella pertussisVirulence Factor P.69 Pertactin

“…For the preparation of WCV or the challenge inoculum, B1586, B1675, and B0613, a Dutch clinical isolate from 1995 expressing P.69 Prn2, were grown in Thijs medium (47). WCV was obtained after heat and formaldehyde inactivation of pelleted bacteria and addition of Al(OH) 3 as an adjuvant.…”