Rational high-throughput system for screening of high sophorolipids-producing strains of Candida bombicola

et al. 2019

Bioresour. Bioprocess.

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Background: A rational and high-performance high-throughput screening system effectively improves the efficiency of strain screening. Results: A rapid and efficient method was developed to detect carbon sources. And then by using glucose analog as screening pressure and two indexes for sophorolipids (SLs) production and glycerol consumption, a mutant of Candida bombicola L1.3 was successfully selected among 3000 mutants. It produces high SLs titer and co-utilizes glucose and glycerol simultaneously. Conclusions: Compared to the wild-type strain, L1.3 exhibited 15.06% higher SLs titer and 35.69% higher glycerol consumption capacity in a 5-L bioreactor. We believe that L1.3 can potentially be used for the efficient industrial production of SLs with simple downstream processing for separating SLs and glycerol.

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: High-throughput Analytical Methods For Preliminary Screeningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity

Lin

et al. 2019

Bioresour. Bioprocess.

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“…The plasma jet generated by ARTP is controlled by the discharged power and gas flow, and its composition mainly includes excited helium atoms, excited oxygen atoms, excited hydrogen atoms, and free •OH radicals ( Zhang et al, 2014 ; Winter et al, 2015 ; Zhang et al, 2015 ; Yu et al, 2019 ). Due to its low voltage radio-frequency power source, low temperature plasma, equal uniform plasma jet, easy operation, and high safety, ARTP has become increasingly popular among microbial breeding operators for its extensive applications ( Wang et al, 2010 ; Zhang et al, 2014 ; Yang et al, 2019 ; Zhou et al, 2019 ). Li et al (2008) have identified the mechanism of radio-frequency and atmospheric-pressure glow discharges (APGD) directly acted on plasmid DNA and oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Acid Protease Activity of Aspergillus oryzae Using Atmospheric and Room Temperature Plasma

Shu

et al. 2020

Front. Microbiol.

Atmospheric and room temperature plasma (ARTP) system is a novel and efficient mutagenesis protocol for microbial breeding. In this study, ARTP was employed to treat spores of Aspergillus oryzae strain 3.042 for selection of high acid protease producers. With an irradiation time of 150 s at the lethal rate of 90%, 19 mutants with higher acid protease activity were initially selected based on different mutant colony morphology and ratio of the clarification halo of protease activity to the colony diameter. Measurements of the acid protease activity revealed that mutant strain B-2 is characterized by a steady hereditary stability with increased acid protease, neutral protease and total protease activities of 54.7, 17.3, and 8.5%, respectively, and decreased alkaline protease activity of 8.1%. In summary, the identified mutant strain B-2 exhibits great potential for the enhancement of the insufficient acid protease activity during the middle and later stages of soy sauce fermentation.

Ultrasound assisted in situ separation of sophorolipids in multi‐phase fermentation system to achieve efficient production by Candida bombicola

Tang

Wang

et al. 2021

Biotechnology Journal

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Background: Sophorolipids (SLs) are regarded as one of the most promising biosurfactants. However, high production costs are the main obstacle to extended SLs application. Semi-continuous fermentation, which is based on in situ separation, is a promising technology for achieving high SLs productivity. Methods and Results:In this study, the sedimentation mechanism of SLs was analyzed.The formation of a hydrophobic mixture of SLs and rapeseed oil was a key factor in sedimentation. And the hydrophobicity and density of the mixture determined SLs sedimentation rate. On this basis, ultrasonic enhanced sedimentation technology (UEST) was introduced, by which the sedimentation rates were increased by 46.9%-485.4% with different ratio of rapeseed oil to SLs. UEST-assisted real-time in situ separation and semi-continuous fermentation were performed. SLs productivity and yield were 2.15 g L −1 h −1 and 0.58 g g −1 , respectively, simultaneously the loss ratio of cells, glucose, and rapeseed oil were significantly reduced. Conclusions:This study provides a new horizon for optimization of the SLs fermentation process.