Rational engineering S1' substrate binding pocket to enhance substrate specificity and catalytic activity of thermal-stable keratinase for efficient keratin degradation

Pei, Xiao-Dong; Fan, He-Liang; Jiao, Dao-Quan; Li, Fan; He, Yi-Ning; Wu, Qing-Ling; Liu, Xiao-Ling; Wang, Cheng-Hua

doi:10.1016/j.ijbiomac.2024.130688

Cited by 2 publications

(1 citation statement)

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“…While researchers predominantly investigate the potential for hair regeneration in AGA, there is limited emphasis on hair quality in areas affected by hair loss. Exposure to sunlight can lead to photodegradation of human hair, with hair photoaging characterized by the lifting of hair cuticles [ 24 ], degradation of keratin [ 35 ], disruption of disulfide bonds [ 36 ], depletion of natural oils, loss of shine, heightened frictional resistance, and compromised mechanical properties [ 37 ]. Based on the findings presented in Figure 1 b, it is evident that there was minimal hair damage in the balding region of AGA, characterized by raised scales, suggesting overall surface structural impairment.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Properties of Platycladus orientalis Flavonoids for Treating UV-Induced Damage in Androgenetic Alopecia Hair

Xu,

Dai,

et al. 2024

Molecules

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Background: Androgenetic alopecia (AGA) causes thinning hair, but poor hair quality in balding areas and damage from UV radiation have been overlooked. Plant extracts like Platycladus orientalis flavonoids (POFs) may improve hair quality in AGA. This study examines POFs’ effectiveness in treating AGA-affected hair and repairing UV-induced damage. Methods: Hair samples were analyzed using scanning electron microscopy (SEM) to examine surface characteristics, electron paramagnetic resonance (EPR) spectroscopy to measure free radicals in the hair, and spectrophotometry to assess changes in hair properties. Results: POFs effectively removed hydroxyl radicals from keratinocytes and had antioxidant properties. They also reduced UV-induced damage to AGA hair by mitigating the production of melanin free radicals. Following POF treatment, the reduction in peroxidized lipid loss in AGA hair was notable at 59.72%, thereby effectively delaying the progression of hair color change. Moreover, protein loss decreased by 191.1 μ/g and tryptophan loss by 15.03%, ultimately enhancing hair’s tensile strength. Conclusion: compared to healthy hair, hair damaged by AGA shows more pronounced signs of damage when exposed to UV radiation. POFs help protect balding hair by reducing oxidative damage and slowing down melanin degradation.

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