Rational design to enhance the catalytic activity of 2-deoxy-D-ribose-5-phosphate aldolase from Pseudomonas syringae pv. syringae B728a

He, Fei-fan; Xin, Yi-yao; Ma, Yuanxin; Yang, Shun Fa; Fang, Hui

doi:10.1016/j.pep.2021.105863

Cited by 2 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 31 ], and Haloarcula japonica [ 32 ]; Thermotoga maritima [ 29 ]; Aciduliprofundum boonei [ 33 ], and Rhodococcus erythropolis [ 34 ]; E . coli [ 29 ], Haemophilus influenzae [ 35 ], and Pseudomonas syringae [ 36 ], were 4.0, 5.5, 6.0, 6.5, 7.0, and 7.5, respectively. The increasing order of temperature values for the optimal activity toward DRP of reported recombinant DERAs from P .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro One-Pot 3-Hydroxypropanal Production from Cheap C1 and C2 Compounds

Seo

Yeom

2022

IJMS

View full text Add to dashboard Cite

One- or two-carbon (C1 or C2) compounds have been considered attractive substrates because they are inexpensive and abundant. Methanol and ethanol are representative C1 and C2 compounds, which can be used as bio-renewable platform feedstocks for the biotechnological production of value-added natural chemicals. Methanol-derived formaldehyde and ethanol-derived acetaldehyde can be converted to 3-hydroxypropanal (3-HPA) via aldol condensation. 3-HPA is used in food preservation and as a precursor for 3-hydroxypropionic acid and 1,3-propanediol that are starting materials for manufacturing biocompatible plastic and polytrimethylene terephthalate. In this study, 3-HPA was biosynthesized from formaldehyde and acetaldehyde using deoxyribose-5-phosphate aldolase from Thermotoga maritima (DERATma) and cloned and expressed in Escherichia coli for 3-HPA production. Under optimum conditions, DERATma produced 7 mM 3-HPA from 25 mM substrate (formaldehyde and acetaldehyde) for 60 min with 520 mg/L/h productivity. To demonstrate the one-pot 3-HPA production from methanol and ethanol, we used methanol dehydrogenase from Lysinibacillus xylanilyticus (MDHLx) and DERATma. One-pot 3-HPA production via aldol condensation of formaldehyde and acetaldehyde from methanol and ethanol, respectively, was investigated under optimized reaction conditions. This is the first report on 3-HPA production from inexpensive alcohol substrates (methanol and ethanol) by cascade reaction using DERATma and MDHLx.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The increasing order of temperature values for the optimal activity toward DRP of reported recombinant DERAs from P . syringae [ 36 ]; H . influenzae [ 35 ]; Yersinia sp.…”

Section: Resultsmentioning

confidence: 99%