Rational Design of and Mechanism Insight into an Efficient Antifreeze Peptide for Cryopreservation

Zinc Cations Uniquely Stabilize Cell Membrane for Cell Cryopreservation

“…Small molecules such as neutral amino acids, trehalose, and glycopeptides have been reported to increase cell viability in cryopreservation, including the notable work of Zhang and Yuan which highlights the importance of protecting the cell membrane as a strategy during the freezing and thawing process. − Metal ions, such as Ca­(II), Mg­(II), Zn­(II), and Cu (II) have been reported to be specifically efficient to protect the cell membranes against lysis. − It has been reported that Zn­(II) can reduce damage caused by reactive oxygen species (ROS) in vitrification, thus improving follicle integrity . However, there is little research on the effect of metal ions on cell membrane protection in cryopreservation.…”