Rational design of a photo-responsive UVR8-derived protein and a self-assembling peptide–protein conjugate for responsive hydrogel formation

Zhang, Xiaoli; Dong, Chunming; Huang, Weixin; Wang, Huaimin; Wang, Ling; Ding, Dan; Zhou, Hao; Long, Jiafu; Wang, Tingliang; Yang, Zhimou

doi:10.1039/c5nr05213k

Cited by 60 publications

(62 citation statements)

References 36 publications

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“…In order to recapitulate these dynamic environments, several materials have been developed, which enable the reversible modulation of mechanical properties in response to chemical or optical stimuli. [3a–c,4] Since light as stimulus offers superior spatiotemporal control compared to classical chemical inducers, materials with reversibly adjustable mechanical properties based on sequential photodegradation and photoinitiated crosslinking, cis – trans isomerization of azobenzene, guest–host interaction of azobenzene and β‐cyclodextrin (all UV and violet light) as well as on the photoreceptors UVR8 (UV light), LOV2 (blue light), or Dronpa (violet and cyan light) were developed. However, materials that allow fast and fully reversible adjustment of mechanical properties under cell culture conditions with cell‐compatible and low energy red light are still lacking.…”

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confidence: 99%

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

et al. 2019

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The mechanical properties of the extracellular environment govern key cellular decision-making processes such as proliferation, differentiation, or migration. [1] Thus, analyzing how cells gauge and interact with their mechanical environment is critical not only for understanding physiological and pathological processes but also for engineering cell and tissue growth and differentiation in regenerative medicine. [2] Although studies using passive elastic or viscoelastic materials have revealed valuable information about cell-matrix interactions, matrices with adjustable mechanical properties more closely reflect the dynamic environments many cells are exposed to in a living organism. [3] In order to recapitulate these dynamic environments, several materials have been developed, which enable the Interrogation and control of cellular fate and function using optogenetics is providing revolutionary insights into biology. Optogenetic control of cells is achieved by coupling genetically encoded photoreceptors to cellular effectors and enables unprecedented spatiotemporal control of signaling processes. Here, a fast and reversibly switchable photoreceptor is used to tune the mechanical properties of polymer materials in a fully reversible, wavelengthspecific, and dose-and space-controlled manner. By integrating engineered cyanobacterial phytochrome 1 into a poly(ethylene glycol) matrix, hydrogel materials responsive to light in the cell-compatible red/far-red spectrum are synthesized. These materials are applied to study in human mesenchymal stem cells how different mechanosignaling pathways respond to changing mechanical environments and to control the migration of primary immune cells in 3D. This optogenetics-inspired matrix allows fundamental questions of how cells react to dynamic mechanical environments to be addressed. Further, remote control of such matrices can create new opportunities for tissue engineering or provide a basis for optically stimulated drug depots. BiomaterialsThe ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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confidence: 99%

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

et al. 2019

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“…Nevertheless, the greatest progress in Vis light reversible reactions for the formation of hydrogels has been made by using photoresponsive proteins. This field has been witnessing important advances in recent years with the demonstration of cell and protein release from hydrogels using the photoresponsive protein UVR8 . Dimers formed by this protein can act as a link between two polypeptide chains that upon UV light irradiation can be cleaved.…”

Section: Hydrogel Scaffold Productionmentioning

confidence: 99%

The Role of Photochemical Reactions in the Development of Advanced Soft Materials for Biomedical Applications

Fernandez‐Villamarin

Brooks

Mendes

2019

Advanced Optical Materials

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In the biomedical field, many innovative materials to improve diagnosis and treatment of diseases are currently being developed. The ability to respond to different stimuli is one of the more interesting properties of such materials. Light, as one of the nature's elementary stimuli, offers an attractive and flexible stimulus for controlling the properties and functions of biomedical‐related materials. As such, photoresponsive systems have been increasingly pursued and finding applications in various biomedical settings, ranging from tissue engineering for the fabrication of bespoke tissue scaffolds to drug delivery, aims at manipulating treatment distribution inside the human body. Efforts have also been devoted to the development of highly efficient methods to control biological activity and bring us closer to mimicking impressive biological actuators. In this progress report, an overview of recent developments in the field is provided and current challenges discussed. Key strategies tailored to address specific issues are examined, offering useful perspectives for future designs.

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“…Due to this combination of enzymatic and chemical reactions, this precursor presented both the higher cellular uptake and cell viability. Besides, when using a photo‐sensitive protein, UVR8 derived protein, as the cross‐linker between the peptide nanofibers, which was based on the homodimer structure with the tax‐interacting protein‐1, this enzyme‐induced hydrogel was capable to present a reversible gel–sol phase transition due to the reversible dimer–monomer transformation of the photo‐sensitive protein . The photo‐controllable hydrogel may raise enormous potential in protein delivery or cells separation.…”

Section: Hydrogels and Nanofibersmentioning

confidence: 99%

Molecular Self‐Assembly Constructed in Physiological Conditions for Cancer Diagnosis and Therapy

Qiao

Wang

2018

Advanced Therapeutics

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For the purpose of cancer theranostics, the performance and potential biosafety of drug/imaging agents remain major challenges. Although passive and active targeting strategies have been reported for effectively enhanced bioimaging or drug delivery performance, researchers are still looking for emerging methods for better targeting, accumulation, and penetration of solid tumors. The strategy for constructing supramolecular self-assemblies in biologically relevant conditions may partially overcome these challenges. Regarding the biomedical applications, supramolecular entities with naturally dynamic and modular properties, are capable of constructing structurally and functionally diversified materials and meeting the requirements of biological complexity. Modules and integrated systems enable response to bio-environment by the modification of their constitution through component exchange or reorganization. In this review, the authors summarize the self-assembled nanosystems (nanoparticles, nanoaggregates, micelles, vesicles, nanofibers, hydrogels, etc.) in physiological/pathological environments and their applications in cancer theranostics. The authors focus on chemical structures, stimuli-responsive bonds, and self-assembly property of nanomaterials, which may offer a guide for the optimal design of self-assembled nanomaterials in clinical cancer treatment and imaging.

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Rational design of a photo-responsive UVR8-derived protein and a self-assembling peptide–protein conjugate for responsive hydrogel formation

Cited by 60 publications

References 36 publications

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

Phytochrome‐Based Extracellular Matrix with Reversibly Tunable Mechanical Properties

The Role of Photochemical Reactions in the Development of Advanced Soft Materials for Biomedical Applications

Molecular Self‐Assembly Constructed in Physiological Conditions for Cancer Diagnosis and Therapy

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