Rational design of a novel acryl-modified CQDs fluorescent probe for highly selective detection and imaging of cysteine in vitro and in vivo

Zhang, Jie; Abdulkhaleq, Ahmed Mohammed Ali; Wang, Jun; Zhou, Xibin

doi:10.1007/s00604-023-05919-4

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2024

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Cited by 2 publications

References 35 publications

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Development of a Dual-Emitting Nanohybrids Ratiometric Fluorescent Probe for Highly Sensitive Detection of L-Cysteine Based on Cu-Ag Alloy Nanoclusters and Carbon Quantum Dots

Wang,

Xue,

zhao

et al. 2024

Preprint

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Development of a Dual-Emitting Nanohybrids Ratiometric Fluorescent Probe for Highly Sensitive Detection of L-Cysteine Based on Cu-Ag Alloy Nanoclusters and Carbon Quantum Dots

Wang,

Xue,

zhao

et al. 2024

Preprint

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A Novel Fluorescence Probe Based on Azamonardine for Detecting and Imaging Cysteine in Cells and Zebrafish with High Selectivity and Sensitivity

Zhao,

Wang,

Abdulkhaleq

et al. 2023

Molecules

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A novel fluorescent probe based on azamonardine (Aza) fluorophore was designed and synthesized for the highly selective detection of cysteine (Cys) in vivo and in vitro. After reacting with acryloyl chloride, the fluorescence of Aza is effectively quenched, resulting in the formation of the Aza-acryl probe. Upon the addition of Cys, the ester bond of Aza-acryl is cleaved, releasing a new compound (Compound 1) with strong fluorescence, thereby achieving fluorescence turn-on detection of Cys. The structure of Aza-acryl was characterized using X-ray crystallography and NMR spectroscopy. Additionally, density functional theory was employed to elucidate the quenching mechanism of the acyl group on the Aza. Aza-acryl exhibits high selectivity towards Cys and distinguishes it from other biothiols such as homocysteine (Hcy) and glutathione (GSH). The mechanism of Aza-acryl for detecting Cys was investigated through HPLC, NMR spectroscopy, high-resolution mass spectrometry, and reaction kinetics experiments. Aza-acryl demonstrates excellent imaging capabilities for Cys in cells and zebrafish, providing a reliable and selectable tool for the detection and imaging of Cys in biological systems.

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Rational design of a novel acryl-modified CQDs fluorescent probe for highly selective detection and imaging of cysteine in vitro and in vivo

Cited by 2 publications

References 35 publications

Development of a Dual-Emitting Nanohybrids Ratiometric Fluorescent Probe for Highly Sensitive Detection of L-Cysteine Based on Cu-Ag Alloy Nanoclusters and Carbon Quantum Dots

Development of a Dual-Emitting Nanohybrids Ratiometric Fluorescent Probe for Highly Sensitive Detection of L-Cysteine Based on Cu-Ag Alloy Nanoclusters and Carbon Quantum Dots

A Novel Fluorescence Probe Based on Azamonardine for Detecting and Imaging Cysteine in Cells and Zebrafish with High Selectivity and Sensitivity

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