Rational design of a chalcogenopyrylium-based surface-enhanced resonance Raman scattering nanoprobe with attomolar sensitivity

Harmsen, Stefan; Bedics, Matthew A.; Wall, Matthew A.; Huang, Ruimin; Detty, Michael R.; Kircher, Moritz F.

doi:10.1038/ncomms7570

Cited by 117 publications

(137 citation statements)

References 46 publications

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“…Recently, chalcogenopyrylium dyes have been developed and found to offer exceptionally strong resonant SERS signals in the NIR spectrum while having high affinity to gold surfaces by having multiple selenium or sulfur groups. 234 . Design and structure of biofunctionalized SERS nanoparticle tags, consisting of a metal nanoparticle core, adsorbed Raman reporters on the metal surface (green stars), a biocompatible layer (orange layer), and targeting ligands.…”

Section: Sers Nanoparticle Tagsmentioning

confidence: 95%

SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging

2015

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Section: Sers Nanoparticle Tagsmentioning

confidence: 95%

SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging

2015

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“…SERS imaging has several key advantages over fluorescence imaging, such as higher sensitivity, unequivocal detection without issues of autofluorescence, and photostability. 19–20 Therefore, SERS imaging could represent an ideal intraoperative method complementary to the whole-body imaging capabilities of MRI and PET, especially in applications where high precision tumor margin delineation is essential. 15, 21–23 …”

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confidence: 99%

Imaging of Liver Tumors Using Surface-Enhanced Raman Scattering Nanoparticles

et al. 2016

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Complete surgical resection is the first-line treatment for most liver malignancies. This goal would be facilitated by an intraoperative imaging method that enables more precise visualization of tumor margins, and detection of otherwise invisible microscopic lesions. To this end, we synthesized silica-encapsulated surface-enhanced Raman scattering (SERS) nanoparticles (NPs) that act as a molecular imaging agent for liver malignancies. We hypothesized that, after intravenous administration, SERS NPs would avidly home to healthy liver tissue, but not to intrahepatic malignancies. We tested these SERS NPs in genetically engineered mouse models of hepatocellular carcinoma and histiocytic sarcoma. After intravenous injection, liver tumors in both models were readily identifiable with Raman imaging. In addition, Raman imaging using SERS NPs enabled detection of microscopic lesions in liver and spleen. We compared the performance of SERS NPs to fluorescence imaging using Indocyanine Green (ICG). We found that SERS NPs delineate tumors more accurately and are less susceptible to photobleaching. Given the known advantages of SERS imaging, namely high sensitivity and specific spectroscopic detection, these findings hold promise for improved resection of liver cancer.

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“…It can also detect premalignant lesions. Most recently, we increased the particle's sensitivity even further, achieving sensitivity in the attomolar range [2]. In this paper I will present an overview over the abilities of these new SERRS nanoparticle generations in preclinical oncological imaging.…”

Section: Introductionmentioning

confidence: 99%

Ultra-high sensitivity imaging of cancer using SERRS nanoparticles

Kircher

2016

SPIE Proceedings

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Surface-enhanced Raman spectroscopy" (SERS) nanoparticles have gained much attention in recent years for in silico, in vitro and in vivo sensing applications. Our group has developed novel generations of biocompatible "surfaceenhanced resonance Raman spectroscopy" (SERRS) nanoparticles as novel molecular imaging agents. Via rigorous optimization of the different variables contributing to the Raman enhancement, we were able to design SERRS nanoparticles with so far unprecedented sensitivity of detection under in vivo imaging conditions (femto-attomolar range). This has resulted in our ability to visualize, with a single nanoparticle, many different cancer types (after intravenous injection) in mouse models. The cancer types we have tested so far include brain, breast, esophagus, stomach, pancreas, colon, sarcoma, and prostate cancer. All mouse models used are state-of-the-art and closely mimic the tumor biology in their human counterparts. In these animals, we were able to visualize not only the bulk tumors, but importantly also microscopic extensions and locoregional satellite metastases, thus delineating for the first time the true extent of tumor spread. Moreover, the particles enable the detection of premalignant lesions. Given their inert composition they are expected to have a high chance for clinical translation, where we envision them to have an impact in various scenarios ranging from early detection, image-guidance in open or minimally invasive surgical procedures, to noninvasive imaging in conjunction with spatially offset (SESORS) Raman detection devices.

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Rational design of a chalcogenopyrylium-based surface-enhanced resonance Raman scattering nanoprobe with attomolar sensitivity

Cited by 117 publications

References 46 publications

SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging

SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging

Imaging of Liver Tumors Using Surface-Enhanced Raman Scattering Nanoparticles

Ultra-high sensitivity imaging of cancer using SERRS nanoparticles

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