Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown

Hasunuma, Tomohisa; Ishii, Jun; Kondo, Akihiko

doi:10.1016/j.cbpa.2015.06.004

Cited by 42 publications

(17 citation statements)

References 62 publications

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“…While more biorefineries are coming online to deliver on the decades old promise of cellulosic ethanol and other green chemicals, the recalcitrance of feedstocks, development of appropriate pre‐treatments, production of appropriate hydrolytic enzymes, and the development of ethanologens able to thrive in the hostile fermentation environment remain significant challenges to the growth and sustainability of this industry. The challenges of various feedstocks, the status quo of pre‐treatment methodologies, and the development of organisms for first‐ and second‐generation biofuels production have been reviewed recently …”

Section: Introductionmentioning

confidence: 99%

“…The yeast Saccharomyces cerevisiae remains the preferred industrial ethanologen due to its high rates of ethanol productivity, high yield, and process robustness, among other desired attributes. Significant progress has been made in engineering strains of S. cerevisiae for cellulose CBP including successful production of the three main types of cellulase activities namely cellobiohydrolase (CBH), endoglucanase (EG), and β‐glucosidase (BGL) and partial conversion of crystalline cellulose to ethanol, but a strain suitable for industrial CBP has not been produced. One significant drawback of this yeast species is its inherently low level of protein secretion.…”

Section: Introductionmentioning

confidence: 99%

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Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

Kroukamp

Haan

Zyl

et al. 2017

Biofuels Bioprod Bioref

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Lignocellulosic biomass remains an attractive feedstock for the production of fuels if a technology can be developed to overcome its recalcitrance. Consolidated bioprocessing (CBP) is one technology under development that aims to make this conversion process economically feasible. While no ideal CBP organism has been developed, several options have been pursued including engineering of the ethanologenic yeast Saccharomyces cerevisiae. Considering the genetic malleability of this model organism, a variety of chemicals and chemical precursors could also be produced directly from cellulosic feedstocks, assuming an enzymatic system for the hydrolysis of the feedstock sugar polymers can be established. While there have been several accounts of the secretion of cellulases by strains of S. cerevisiae and the successful conversion of limited amounts of amorphous and model crystalline cellulose feedstocks to ethanol, substantial conversion of crystalline cellulose by these strains in the absence of exogenous cellulases has not been reported. The most cited reasons for this were the low secretion titer of cellulases in general and of cellobiohydrolases in particular. This review will compare the efforts that have been made to enhance heterologous protein secretion in the yeast S. cerevisiae through rational strain engineering with a focus on cellulases and will investigate important factors in developing successful CBP-yeast strains.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

Kroukamp

Haan

Zyl

et al. 2017

Biofuels Bioprod Bioref

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“…These attributes are expected to contribute to cost reduction and to facilitate commercial viability [29]. Therefore, the co-displaying of β-mannanase and β-mannosidase on the yeast cell surface is a promising strategy applicable to yeast fermentations designed to produce other (non-ethanol) biofuels and biochemicals in metabolically engineered S. cerevisiae [30–32]. In future studies, the additional display of other enzymes (such as glucosidase and galactosidase) on the mannanase- and mannosidase-co-displaying yeast cells is expected to enable the fermentation of glucomannans and galactoglucomannans.…”

Section: Discussionmentioning

confidence: 99%

From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae

Ishii

Okazaki

Djohan

et al. 2016

Biotechnol Biofuels

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BackgroundMannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays β-mannanase and β-mannosidase; this strain is expected to facilitate ethanol fermentation using mannan as a biomass source.ResultsParental yeast S. cerevisiae assimilated mannose and glucose as monomeric sugars, producing ethanol from mannose. We constructed yeast strains that express tethered β-mannanase and β-mannosidase; co-display of the two enzymes on the cell surface was confirmed by immunofluorescence staining and enzyme activity assays. The constructed yeast cells successfully hydrolyzed 1,4-β-d-mannan and produced ethanol by assimilating the resulting mannose without external addition of enzymes. Furthermore, the constructed strain produced ethanol from 1,4-β-d-mannan continually during the third batch of repeated fermentation. Additionally, the constructed strain produced ethanol from ivory nut mannan; ethanol yield was improved by NaOH pretreatment of the substrate.ConclusionsWe successfully displayed β-mannanase and β-mannosidase on the yeast cell surface. Our results clearly demonstrate the utility of the strain co-displaying β-mannanase and β-mannosidase for ethanol fermentation from mannan biomass. Thus, co-tethering β-mannanase and β-mannosidase on the yeast cell surface provides a powerful platform technology for yeast fermentation toward the production of bioethanol and other biochemicals from lignocellulosic materials containing mannan components.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0600-4) contains supplementary material, which is available to authorized users.

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“…K) regarding the systemwide modification of intracellular metabolic pathways to improve the biorefinery manufacturing processes (Hasunuma et al . ; Kawaguchi et al . ) (day 5); (ix) ‘Glycomics analysis of protein glycosylation and its applications in yeast’ by Dr. Hyun Ah Kang (Chung‐Ang University, Korea; Fig.…”

Section: Plenary Lecturesmentioning

confidence: 99%

Yeasts for Global Happiness: report of the 14th International Congress on Yeasts (ICY14) held in Awaji Island

Watanabe

2017

Genes to Cells

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The 14th International Congress on Yeasts (ICY14) was held at Awaji Yumebutai International Conference Center (Awaji, Hyogo) in Japan from 11 to 15 September 2016. The main slogan of ICY14 was ‘Yeasts for Global Happiness’, which enabled us to acknowledge the high‐potential usefulness of yeasts contributing to the global happiness in terms of food/beverage, health/medicine and energy/environment industries, as well as to basic biosciences. In addition, two more concepts were introduced: ‘from Japan to the world’ and ‘from senior to junior’. As it was the first ICY meeting held in Japan or other Asian countries, ICY14 provided a good opportunity to widely spread the great achievements by Japanese and Asian yeast researchers, such as those by the 2016 Nobel Laureate Dr. Yoshinori Ohsumi, and also, to convey the fun and importance of yeasts to the next generation of researchers from Asia and all over the world. As a result, a total of 426 yeast lovers from 42 countries (225 overseas and 201 domestic participants) with different generations attended ICY14 to share the latest knowledge of a wide range of yeast research fields and to join active and constructive scientific discussions.

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Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown

Cited by 42 publications

References 62 publications

Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae

From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae

Yeasts for Global Happiness: report of the 14th International Congress on Yeasts (ICY14) held in Awaji Island

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