Ratiometric ultrasensitive electrochemical immunosensor based on redox substrate and immunoprobe

Tang, Zhongxue; Ma, Zhanfang

doi:10.1038/srep35440

Cited by 18 publications

(10 citation statements)

References 44 publications

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“…The redox substrate, which facilitates a strong current signal, has paved the way to enhance the analytical performance [53]. GO was used to increase the electrical conductivity on the surface and the stability of the 3-D porous structure, resulting in dual signal enhancement [54].…”

Section: Graphenementioning

confidence: 99%

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

2020

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Background: The electrochemical biosensor is one of the typical sensing devices based on transducing the biochemical events to electrical signals. In this type of sensor, an electrode is a key component that is employed as a solid support for immobilization of biomolecules and electron movement. Thanks to numerous nanomaterials that possess the large surface area, synergic effects are enabled by improving loading capacity and the mass transport of reactants for achieving high performance in terms of analytical sensitivity. Main body: We categorized the current electrochemical biosensors into two groups, carbon-based (carbon nanotubes and graphene) and non-carbon-based nanomaterials (metallic and silica nanoparticles, nanowire, and indium tin oxide, organic materials). The carbon allotropes can be employed as an electrode and supporting scaffolds due to their large active surface area as well as an effective electron transfer rate. We also discussed the non-carbon nanomaterials that are used as alternative supporting components of the electrode for improving the electrochemical properties of biosensors. Conclusion: Although several functional nanomaterials have provided the innovative solid substrate for high performances, developing on-site version of biosensor that meets enough sensitivity along with high reproducibility still remains a challenge. In particular, the matrix interference from real samples which seriously affects the biomolecular interaction still remains the most critical issues that need to be solved for practical aspect in the electrochemical biosensor. Recently, various electrochemistry-driven biosensing methods have been introduced for simple and miniaturized analytical devices for on-site analysis. This trend can be applied to replace the commercial lab instruments manufactured by the renowned in vitro diagnosis

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Section: Graphenementioning

confidence: 99%

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

2020

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“…Metal ions possess good electrochemical redox activity, which makes them ideal materials as electrochemical signal substances [ 56 , 57 ]. With the advantages of easy accessibility and generated sharp electrochemical signal peaks, metal ions like silver ions, copper(II) ions, lead(II) ions usually serve as the excellent labels to indicate the content of multiple biomarkers [ 58 , 59 , 60 , 61 ]. Our group has come up with a method based on the chip-like GCE composed of three electrodes connected in a parallel for simultaneous detection [ 43 ].…”

Section: Electrochemical Signal Substances For Multiplexed Immunoassaymentioning

confidence: 99%

Electrochemical Signal Substance for Multiplexed Immunosensing Interface Construction: A Mini Review

Feng¹,

Chu²,

Ma³

2022

Molecules

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Appropriate labeling method of signal substance is necessary for the construction of multiplexed electrochemical immunosensing interface to enhance the specificity for the diagnosis of cancer. So far, various electrochemical substances, including organic molecules, metal ions, metal nanoparticles, Prussian blue, and other methods for an electrochemical signal generation have been successfully applied in multiplexed biosensor designing. However, few works have been reported on the summary of electrochemical signal substance applied in constructing multiplexed immunosensing interface. Herein, according to the classification of labeled electrochemical signal substance, this review has summarized the recent state-of-art development for the designing of electrochemical immunosensing interface for simultaneous detection of multiple tumor markers. After that, the conclusion and prospects for future applications of electrochemical signal substances in multiplexed immunosensors are also discussed. The current review can provide a comprehensive summary of signal substance selection for workers researched in electrochemical sensors, and further, make contributions for the designing of multiplexed electrochemical immunosensing interface with well signal.

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“…Metal NP sandwich-style sensors can improve selectivity combining the selectivity of antibodies, with the transducing properties of NPs. Tang and Ma constructed a sandwich assay for the detection of immunoglobulin G (IgG) using two functionalised AuNPs (Figure 36) [117]. Firstly, a GCE was functionalised with a carboxymethyl cellulose-Au-Pb 2+ nanocomposite, then labelled with the recognition antibody 1 (Ab1).…”

Section: Antibodiesmentioning

confidence: 99%

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Spring

Goggins²,

Frost

2021

Molecules

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Electrochemical biosensors are an increasingly attractive option for the development of a novel analyte detection method, especially when integration within a point-of-use device is the overall objective. In this context, accuracy and sensitivity are not compromised when working with opaque samples as the electrical readout signal can be directly read by a device without the need for any signal transduction. However, electrochemical detection can be susceptible to substantial signal drift and increased signal error. This is most apparent when analysing complex mixtures and when using small, single-use, screen-printed electrodes. Over recent years, analytical scientists have taken inspiration from self-referencing ratiometric fluorescence methods to counteract these problems and have begun to develop ratiometric electrochemical protocols to improve sensor accuracy and reliability. This review will provide coverage of key developments in ratiometric electrochemical (bio)sensors, highlighting innovative assay design, and the experiments performed that challenge assay robustness and reliability.

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Ratiometric ultrasensitive electrochemical immunosensor based on redox substrate and immunoprobe

Cited by 18 publications

References 44 publications

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

Electrochemical Signal Substance for Multiplexed Immunosensing Interface Construction: A Mini Review

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

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