Ratiometric Nanoparticle Probe Based on FRET‐Amplified Phosphorescence for Oxygen Sensing with Minimal Phototoxicity

Ashokkumar, Pichandi; Adarsh, Nagappanpillai; Klymchenko, Andrey S.

doi:10.1002/smll.202002494

Cited by 47 publications

(34 citation statements)

References 98 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 11,40–42 ] At the same time, their size can be tuned over a wide range from less than 10 to over 50 nm, and they can be endowed with stealth properties through the chemistry of the polymer and the conditions used for particle assembly. [ 11,12,41–44 ] Such particles have already found various applications, including multicolor labeling of cells in vitro and in vivo, [ 45 ] single‐particle tracking in cells [ 11,12 ] and mice brain, [ 46 ] and oxygen sensing, [ 47 ] as well as the detection of DNA and RNA down to the single‐particle and single‐molecule level. [ 36,42,48 ]…”

Section: Introductionmentioning

confidence: 99%

Size‐Dependent Electroporation of Dye‐Loaded Polymer Nanoparticles for Efficient and Safe Intracellular Delivery

et al. 2020

Self Cite

View full text Add to dashboard Cite

Efficient and safe delivery of nanoparticles (NPs) into the cytosol of living cells constitutes a major methodological challenge in bio‐nanotechnology. Electroporation allows direct transfer of NPs into the cytosol by forming transient pores in the cell membrane, but it is criticized for invasiveness, and the applicable particle sizes are not well defined. Here, in order to establish principles for efficient delivery of NPs into the cytosol with minimal cytotoxicity, the influence of the size of NPs on their electroporation and intracellular behavior is investigated. For this study, fluorescent dye‐loaded polymer NPs with core sizes between 10 and 40 nm are prepared. Optimizing the electroporation protocol allows minimizing contributions of endocytosis and to study directly the effect of NP size on electroporation. NPs of <20 nm hydrodynamic size are efficiently delivered into the cytosol, whereas this is not the case for NPs of >30 nm. Moreover, only particles of core size <15 nm diffuse freely throughout the cytosol. While electroporation at excessive electric fields induces cytotoxicity, the use of small NPs <20 nm allows efficient delivery at mild electroporation conditions. These results give clear methodological and design guidelines for the safe delivery of NPs for intracellular applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Size‐Dependent Electroporation of Dye‐Loaded Polymer Nanoparticles for Efficient and Safe Intracellular Delivery

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The luminescence levels could be easily determined by implementing an optical fiber-based detector or coupling with a very simple optical setup. Recent advancements in the development of oxygen-sensing nanoparticles 50 could further increase the sensitivity of the oxygen-based sensing platforms and allow us to simplify the optical setup for luminescence quantification.…”

Section: Discussionmentioning

confidence: 99%

Real-Time Respiration Changes as a Viability Indicator for Rapid Antibiotic Susceptibility Testing in a Microfluidic Chamber Array

et al. 2021

View full text Add to dashboard Cite

Rapid identification of a pathogen and the measurement of its antibiotic susceptibility are key elements in the diagnostic process of bacterial infections. Microfluidic technologies offer great control over handling and manipulation of low sample volumes with the possibility to study microbial cultures on the single-cell level. Downscaling the dimensions of cultivation systems directly results in a lower number of bacteria required for antibiotic susceptibility testing (AST) and thus in a reduction of the time to result. The developed platform presented in this work allows the reading of pathogen resistance profiles within 2–3 h based on the changes of dissolved oxygen levels during bacterial cultivation. The platform contains hundreds of individual growth chambers prefilled with a hydrogel containing oxygen-sensing nanoprobes and different concentrations of antibiotic compounds. The performance of the developed platform is tested using quality control Escherichia coli strains (ATCC 25922 and ATCC 35218) in response to clinically relevant antibiotics. The results are in agreement with values given in reference guidelines and independent measurements using a clinical AST protocol. Finally, the platform is successfully used for the AST of an E. coli clinical isolate obtained from a patient blood culture.

show abstract

“…The luminescence levels could be easily determined by implementing an optical fiber-based detector or coupling with a very simple optical setup. The recent advancement in the oxygen sensing nanoparticles 43 could further increase the sensitivity of the oxygen-based sensing platforms and allow to simplify the optical setup for the luminescence quantification.…”

Section: Discussionmentioning

confidence: 99%

Real-time respiration changes as a viability indicator for rapid antibiotic susceptibility testing in a microfluidic chamber array

Jusková

Schmitt

Kling

et al. 2021

Preprint

View full text Add to dashboard Cite

Rapid identification of a pathogen and the measurement of its antibiotic susceptibility are key elements in the diagnostic process of bacterial infections. Microfluidic technologies offer great control over handling and manipulation of low sample volumes with the possibility to study microbial cultures on the single-cell level. Downscaling the dimensions of cultivation systems directly results in a lower number of bacteria required for antibiotic susceptibility testing (AST) and thus in a reduction of the time to result. The developed platform presented in this work allows the reading of pathogen resistance profiles within 2-3 hours based on the changes of the dissolved oxygen levels during bacterial cultivation. The platform contains hundreds of individual growth chambers prefilled with a hydrogel containing oxygen-sensing nanoprobes and different concentrations of antibiotic compounds. The performance of the microfluidic platform is tested using quality control Escherichia coli strains (ATCC 25922 and ATCC 35218) in response to different clinically relevant antibiotics. The achieved results are in agreement with values given in clinical reference guides and independent measurements using a clinical AST protocol. Finally, the platform is successfully used for AST of an E. coli clinical isolate obtained from a patient blood culture.

show abstract

Ratiometric Nanoparticle Probe Based on FRET‐Amplified Phosphorescence for Oxygen Sensing with Minimal Phototoxicity

Abstract: Luminescent oxygen probes enable direct imaging of hypoxic conditions in cells and tissues, which are associated with variety of diseases, including cancer. Here, we Received: ((will be filled in by the editorial staff)) Revised: ((will be filled in by the editorial staff))

Cited by 47 publications

References 98 publications

Size‐Dependent Electroporation of Dye‐Loaded Polymer Nanoparticles for Efficient and Safe Intracellular Delivery

Size‐Dependent Electroporation of Dye‐Loaded Polymer Nanoparticles for Efficient and Safe Intracellular Delivery

Real-Time Respiration Changes as a Viability Indicator for Rapid Antibiotic Susceptibility Testing in a Microfluidic Chamber Array

Real-time respiration changes as a viability indicator for rapid antibiotic susceptibility testing in a microfluidic chamber array

Contact Info

Product

Resources

About