Ratiometric fluorescence enzyme-linked immunosorbent assay based on carbon dots@SiO₂@CdTe quantum dots with dual functionalities for alpha-fetoprotein

Abstract

Molecular tags such as fluorophores are increasingly being replaced with nanoparticles thanks to their superior optical properties, substantially chemical stability, and stability against photobleaching. Herein, we innovatively constructed a new...

The construction of dual-emissive ratiometric fluorescent probes based on fluorescent nanoparticles for the detection of metal ions and small molecules

“…SiO 2 NPs with the advantages of easy preparation, stability, and optical transparency are the commonly used core. 98–103 Core-satellite-structured dye@SiO 2 @CDs for the detection of Cu 2+ , 99 CDs@SiO 2 @CdTe QDs for the detection of H 2 O 2 -related chemical reactions, 100 r-CdTe QDs@SiO 2 @g-CdTe QDs for the detection of aspirin, 101 CDs@SiO 2 /AuNCs for the detection of Cu 2+ , 102 and CDs@SiO 2 @AuNCs for the detection of Ag + (ref. 103) have been reported.…”

Electrochemical Sensor Based on Nanomaterials and Its Application in the Detection of Alpha Fetoprotein

“…AFP threshold is 20 ng/mL [10]. To date, many methods have been used to measure AFP, including mass spectrometry [11], fluorescence [12], chemiluminescence assay [13], enzyme-linked immunosorbent assay [14], radioimmunoassay [15], and electrochemistry. Among these strategies, electrochemical immunoassays have been widely developed and are the prefer technique to quantitatively detect AFP, due to their inherent advantages, including low detection lines, low-cost ease of handling, and small amounts of analytes.…”

Photoelectrochemical immunoassay of alpha-fetoprotein based on a SnO2/In2S3 heterojunction and an enzyme-catalyzed precipitation strategy

“…3 Serum AFP levels are usually lower than 25 ng mL −1 in healthy adults, but are notably higher in patients with liver cancer and continue to increase with the disease progress. 4 To date, many analytical methods have been established for AFP assay, such as enzyme-linked immunosorbent assay, 5 electrochemiluminescence immunoassay, 6 fluorescence assay, 7 and electrochemical assay, 8 each with its advantages and disadvantages. Obviously, it is an interesting and important topic to develop simple, accurate and sensitive analytical methods for AFP assays.…”