Ratiometric electrochemical lateral flow immunoassay for the detection of Streptococcus suis serotype 2

Kunpatee, Kanjana; Khantasup, Kannika; Komolpis, Kittinan; Yakoh, Abdulhadee; Nuanualsuwan, Suphachai; Sain, Mohini M.; Chaiyo, Sudkate

doi:10.1016/j.bios.2023.115742

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…Researchers have made many innovative efforts to improve the sensitivity of LFIA, such as inventing new reporter particles [ 12 , 13 ], optimizing membrane properties [ 14 , 15 ], improving experimental reagents [ 16 , 17 ], modifying LFIA geometry [ 18 , 19 , 20 ], and using different technologies [ 21 , 22 , 23 ]. These innovations have indeed enhanced the detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow

Zhao,

Zhang,

Niu

et al. 2024

Sensors

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Lateral flow immunoassay (LFIA) is extensively utilized for point-of-care testing due to its ease of operation, cost-effectiveness, and swift results. This study investigates the flow dynamics and reaction mechanisms in LFIA by developing a three-dimensional model using the Richards equation and porous media transport, and employing numerical simulations through the finite element method. The study delves into the transport and diffusion behaviors of each reaction component in both sandwich LFIA and competitive LFIA under non-uniform flow conditions. Additionally, the impact of various parameters (such as reporter particle concentration, initial capture probe concentrations for the T-line and C-line, and reaction rate constants) on LFIA performance is analyzed. The findings reveal that, in sandwich LFIA, optimizing parameters like increasing reporter particle concentration and initial capture probe concentration for the T-line, as well as adjusting reaction rate constants, can effectively enhance detection sensitivity and broaden the working range. Conversely, in competitive LFIA, the effects are inverse. This model offers valuable insights for the design and enhancement of LFIA assays.

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Section: Introductionmentioning

confidence: 99%

Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow

Zhao,

Zhang,

Niu

et al. 2024

Sensors

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“…On the other hand, increasing multiple signal outputs of LFIA to obtain accurate quantitative results. At present, the signals of LFIA mainly include fluorescent, Raman response, electrochemical, and photothermal response . Among them, the incorporation of photothermal response into LFIA (PLFIA) can yield the following advantages: (i) PLFIA requires only a portable infrared thermal imager and a laser source for data capture, which is a user-friendly device to bring convenience.…”

Section: Introductionmentioning

confidence: 99%

Layer-By-Layer Designed Spark-Type AuCuPt Alloy with Robust Broadband Absorption to Enhance Sensitivity in Flexible Detection of Estriol by a Lateral Flow Immunoassay

Wu,

Yin,

Cheng

et al. 2024

Anal. Chem.

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“…The multimode LFIA detection can output signals of different functions through nanomaterials (quantum dots, nanomases, metal–organic frameworks, , etc.) and specific antibodies (monoclonal antibodies, polyclonal antibodies, nanoantibodies), which mainly include colorimetric signal, fluorescence signal, photothermal signal, catalytic signal, electrochemical signal and surface-enhanced Raman scattering (SERS) signal, providing more sensitive quantitative analysis results. The multimode signal output was mainly obtained by the excellent characteristics of nanomaterials themselves, as an important part of LFIA, nanomaterials directly affected the sensitivity of detection. ,, Therefore, it was imperative to develop a multifunctional nanomaterial for the LFIA to improve the sensitivity.…”

mentioning

confidence: 99%

“Cave Effect” Induces Self-Assembled Bimetallic Hollow Structure for Three-in-One Lateral Flow Immunoassay

Wu,

Xi,

et al. 2024

Nano Lett.

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Bimetallic hollow structures have attracted much attention due to their unique properties, but they still face the problems of nonuniform alloys and excessive etching leading to structural collapse. Here, uniform bimetallic hollow nanospheres are constructed by pore engineering and then highly loaded with hemin (Hemin@MOF). Interestingly, in the presence of polydopamine (PDA), the competitive coordination between anionic polymer (γ-PGA) and dimethylimidazole does not lead to the collapse of the external framework but self-assembly into a hollow structure. By constructing the Hemin@MOF immune platform and using E. coli O157:H7 as the detection object, we find that the visual detection limits can reach 10, 3, and 3 CFU/mL in colorimetric, photothermal, and catalytic modes, which is 4 orders of magnitude lower than the traditional gold standard. This study provides a new idea for the morphological modification of the metal−organic skeleton and multifunctional immunochromatography detection.

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Ratiometric electrochemical lateral flow immunoassay for the detection of Streptococcus suis serotype 2

Cited by 6 publications

References 36 publications

Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow

Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow

Layer-By-Layer Designed Spark-Type AuCuPt Alloy with Robust Broadband Absorption to Enhance Sensitivity in Flexible Detection of Estriol by a Lateral Flow Immunoassay

“Cave Effect” Induces Self-Assembled Bimetallic Hollow Structure for Three-in-One Lateral Flow Immunoassay

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