Ratiometric electrochemical biosensor based on silver nanoparticles coupled with walker amplification for sensitive detection of microRNA

Li, Hongkun; Cai, Qianqian; Yan, Xiaoshi; Jie, Guifen; Jie, Guitao

doi:10.1016/j.snb.2021.131115

Cited by 19 publications

(5 citation statements)

References 30 publications

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“… 79 Ag NPs can also be used as nano-signal probes to improve sensitivity. For example, a novel ratio electrochemical biosensor based on Ag NPs has recently been developed for the detection of miRNA 80 ( Fig. 6C ).…”

Section: Advantages and Disadvantages Of Biosensors Based On Nanomate...mentioning

confidence: 99%

Nanomaterials promote the fast development of electrochemical MiRNA biosensors

Ouyang,

Huang,

et al. 2024

RSC Adv.

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Section: Advantages and Disadvantages Of Biosensors Based On Nanomate...mentioning

confidence: 99%

Nanomaterials promote the fast development of electrochemical MiRNA biosensors

Ouyang,

Huang,

et al. 2024

RSC Adv.

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“…Streptavidin-modified AgNPs interact with biotin on HP2, generating a peak current proportional to the miR-21 concentration (LOD 0.4 fM). A similar but more complex amplification approach exploiting AgNPs' properties was illustrated in a very recent work [134]. A Methylene blue (MB) DNA hairpin (MB-HP1) and an AgNPs-DNA hairpin (Ag-HP2) were employed as reference and signal probes, respectively.…”

Section: Silver Nanoparticlesmentioning

confidence: 99%

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Martino

Tammaro

Misso

et al. 2023

IJMS

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MicroRNA (miRNA) are constituted of approximately 22 nucleotides and play an important role in the regulation of many physiological functions and diseases. In the last 10 years, an increasing interest has been recorded in studying the expression profile of miRNAs in cancer. Real time-quantitative polymerase chain reaction (RT-qPCR), microarrays, and small RNA sequencing represent the gold standard techniques used in the last 30 years as detection methods. The advent of nanotechnology has allowed the fabrication of nanostructured biosensors which are widely exploited in the diagnostic field. Nanostructured biosensors offer many advantages: (i) their small size allows the construction of portable, wearable, and low-cost products; (ii) the large surface–volume ratio enables the loading of a great number of biorecognition elements (e.g., probes, receptors); and (iii) direct contact of the recognition element with the analyte increases the sensitivity and specificity inducing low limits of detection (LOD). In this review, the role of nanostructured biosensors in miRNA detection is explored, focusing on electrochemical and optical sensing. In particular, four types of nanomaterials (metallic nanoparticles, graphene oxide, quantum dots, and nanostructured polymers) are reported for both detection strategies with the aim to show their distinct properties and applications.

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“…AgNPs have found application as sensors in electrochemical and optical detection due to their high extinction coefficient, high scattering-to-extinction ratio, and high field enhancement. AgNPs show distinct amplified signals [ 18 ] along with strong Raman and fluorescence enhancement. Successful approaches are based on plasmonic properties of AgNPs implemented in platforms applying SERS [ 19 ], LSPR [ 20 ], or fluorescence readouts for detecting miRNA [ 21 ].…”

Section: Nanomaterials Used As Substratesmentioning

confidence: 99%

“…A complementary DNA probe modified with SH can be covalently bonded to the AuNP surface [ 14 , 29 , 41 ], and similarly, the same type of modification can be used for a conjugation on AgNPs [ 18 , 69 ], core–shell Au–Ag NPs [ 26 ], Au–Ag nanocages [ 20 ], AuNP–peptide nanotube composites [ 12 ], Au-coated conglomerates of superparamagnetic NPs [ 43 ], citrate-capped AuNPs electrostatically adsorbed to Fe 3 O 4 NPs [ 70 ], Ag nanoclusters on Au electrode [ 71 ], and hollow Au–Ag nanospheres [ 72 ].…”

Section: Conjugation Strategies Leading To Functionalizationmentioning

confidence: 99%

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

Vilímová¹,

Hervé-Aubert²,

Chourpa³

2022

Molecules

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Recently, microRNAs (miRNA) captured the interest as novel diagnostic and prognostic biomarkers, with their potential for early indication of numerous pathologies. Since miRNA is a short, non-coding RNA sequence, the sensitivity and selectivity of their detection remain a cornerstone of scientific research. As such, methods based on nanomaterials have emerged in hopes of developing fast and facile approaches. At the core of the detection method based on nanotechnology lie nanoprobes and other functionalized nanomaterials. Since miRNA sensing and detection are generally rooted in the capture of target miRNA with the complementary sequence of oligonucleotides, the sequence needs to be attached to the nanomaterial with a specific conjugation strategy. As each nanomaterial has its unique properties, and each conjugation approach presents its drawbacks and advantages, this review offers a condensed overview of the conjugation approaches in nanomaterial-based miRNA sensing. Starting with a brief recapitulation of specific properties and characteristics of nanomaterials that can be used as a substrate, the focus is then centered on covalent and non-covalent bonding chemistry, leading to the functionalization of the nanomaterials, which are the most commonly used in miRNA sensing methods.

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Ratiometric electrochemical biosensor based on silver nanoparticles coupled with walker amplification for sensitive detection of microRNA

Cited by 19 publications

References 30 publications

Nanomaterials promote the fast development of electrochemical MiRNA biosensors

Nanomaterials promote the fast development of electrochemical MiRNA biosensors

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

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