Ratiometric Detection of Nerve Agents by Coupling Complementary Properties of Silicon-Based Quantum Dots and Green Fluorescent Protein

Robidillo, Christopher Jay T.; Wandelt, Sophia; Dalangin, Rochelin; Zhang, Lijuan; Yu, Haoyang; Meldrum, A.; Campbell, Robert E.; Veinot, Jonathan G. C.

doi:10.1021/acsami.9b10996

Cited by 31 publications

(48 citation statements)

References 58 publications

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“…This nanosensor was successfully applied for ratiometric imaging of Hg 2+ in HeLa cells. To date, SiND-associated optical applications have focused on bioimaging [52,53], while relatively little work has touched on their biosensing aspects [54]. The application of SiNDs in biosensing should be further explored.…”

Section: Silicon Nanomaterial-based Ratiometric Fluorescent Biosensorsmentioning

confidence: 99%

Nanomaterial-Based Dual-Emission Ratiometric Fluorescent Sensors for Biosensing and Cell Imaging

et al. 2021

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Owing to the unique optophysical properties of nanomaterials and their self-calibration characteristics, nanomaterial-based (e.g., polymer dots (Pdots) quantum dots (QDs), silicon nanorods (SiNRs), and gold nanoparticle (AuNPs), etc.) ratiometric fluorescent sensors play an essential role in numerous biosensing and cell imaging applications. The dual-emission ratiometric fluorescence technique has the function of effective internal referencing, thereby avoiding the influence of various analyte-independent confounding factors. The sensitivity and precision of the detection can therefore be greatly improved. In this review, the recent progress in nanomaterial-based dual-emission ratiometric fluorescent biosensors is systematically summarized. First, we introduce two general design approaches for dual-emission ratiometric fluorescent sensors, involving ratiometric fluorescence with changes of one response signal and two reversible signals. Then, some recent typical examples of nanomaterial-based dual-emission ratiometric fluorescent biosensors are illustrated in detail. Finally, probable challenges and future outlooks for dual-emission ratiometric fluorescent nanosensors for biosensing and cell imaging are rationally discussed.

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Section: Silicon Nanomaterial-based Ratiometric Fluorescent Biosensorsmentioning

confidence: 99%

Nanomaterial-Based Dual-Emission Ratiometric Fluorescent Sensors for Biosensing and Cell Imaging

et al. 2021

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“…Combining the SiNCs and mAmetrine1.2 (a protein variant), a SiNCs sensor was produced for p-nitrophenyl-containing organophosphate nerve agents paraoxon (PX) and parathion (PT). This sensor in the form of paper exhibited high sensibility such that the detection limitation for the concentration was as small as 5 μΜ (Figure 7A) (Robidillo et al, 2019). Besides the sensors for direct detection, some other sensors based on SiNCs synthesized from thermal disproportionation required multiple steps for detection.…”

Section: Sensormentioning

confidence: 99%

Thermal Disproportionation for the Synthesis of Silicon Nanocrystals and Their Photoluminescent Properties

Wang

Hong

et al. 2021

Front. Chem.

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In the past decades, silicon nanocrystals have received vast attention and have been widely studied owing to not only their advantages including nontoxicity, high availability, and abundance but also their unique luminescent properties distinct from bulk silicon. Among the various synthetic methods of silicon nanocrystals, thermal disproportionation of silicon suboxides (often with H as another major composing element) bears the superiorities of unsophisticated equipment requirements, feasible processing conditions, and precise control of nanocrystals size and structure, which guarantee a bright industrial application prospect. In this paper, we summarize the recent progress of thermal disproportionation chemistry for the synthesis of silicon nanocrystals, with the focus on the effects of temperature, Si/O ratio, and the surface groups on the resulting silicon nanocrystals’ structure and their corresponding photoluminescent properties. Moreover, the paradigmatic application scenarios of the photoluminescent silicon nanocrystals synthesized via this method are showcased or envisioned.

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“…Up to now, various 0D nanomaterials have been extensively explored. For instance, graphene quantum dots (GQDs) (Qian et al, 2014;Lu et al, 2018;Yan et al, 2019), carbon quantum dots (CQDs) (Zheng et al, 2015;Li et al, 2018b;Pandit et al, 2019), fullerenes (Barberis et al, 2015;, inorganic quantum dots (QDs) (Freeman et al, 2012;Li et al, 2018a;Robidillo et al, 2019), magnetic nanoparticles (MNPs) (Yu et al, 2017;Bragina et al, 2019), noble metal nanoparticles (Yang C. T. et al, 2016;Jain and Chauhan, 2017;Bagheri et al, 2018), upconversion nanoparticles (UCNPs) (Cheng Z. H. et al, 2019;Gu et al, 2019) and polymer dots (Pdots) (Ou et al, 2019; are all representative 0D nanomaterials with potential applications in materials science (Zhang X. et al, 2014), photovoltaic science (Guo et al, 2010), catalysis (Xu et al, 2015), energy (Guldi and Sgobba, 2011), sensing (Ramanathan et al, 2019), biomedicine (Yao et al, 2018) and nanodevices (Zhang Y. et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Application of Zero-Dimensional Nanomaterials in Biosensing

et al. 2020

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Zero-dimensional (0D) nanomaterials, including graphene quantum dots (GQDs), carbon quantum dots (CQDs), fullerenes, inorganic quantum dots (QDs), magnetic nanoparticles (MNPs), noble metal nanoparticles, upconversion nanoparticles (UCNPs) and polymer dots (Pdots), have attracted extensive research interest in the field of biosensing in recent years. Benefiting from the ultra-small size, quantum confinement effect, excellent physical and chemical properties and good biocompatibility, 0D nanomaterials have shown great potential in ion detection, biomolecular recognition, disease diagnosis and pathogen detection. Here we first introduce the structures and properties of different 0D nanomaterials. On this basis, recent progress and application examples of 0D nanomaterials in the field of biosensing are discussed. In the last part, we summarize the research status of 0D nanomaterials in the field of biosensing and anticipate the development prospects and future challenges in this field.

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Ratiometric Detection of Nerve Agents by Coupling Complementary Properties of Silicon-Based Quantum Dots and Green Fluorescent Protein

Cited by 31 publications

References 58 publications

Nanomaterial-Based Dual-Emission Ratiometric Fluorescent Sensors for Biosensing and Cell Imaging

Nanomaterial-Based Dual-Emission Ratiometric Fluorescent Sensors for Biosensing and Cell Imaging

Thermal Disproportionation for the Synthesis of Silicon Nanocrystals and Their Photoluminescent Properties

Application of Zero-Dimensional Nanomaterials in Biosensing

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