RatioCRISPR: A ratiometric biochip based on CRISPR/Cas12a for automated and multiplexed detection of heteroplasmic SNPs in mitochondrial DNA

Wu, Xiaolong; Zhao, Yi; Guo, Chuanghao; Liu, Conghui; Zhang, Qianling; Chen, Yong; Liu, Yizhen; Zhang, Xueji

doi:10.1016/j.bios.2023.115676

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Lateral Flow Technology1

Strategies For Multiplexed Detection By Crispr-cas System1

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2024

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Cited by 6 publications

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“…Wu et al proposed an automated and multiplexed CRISPR-Cas12a ratiometric biochip sensor (RatioCRISPR) to detect the heteroplasmy level of mtDNA diseases. 101 DNA was extracted and mixed with RPA reagent and then added to the chip from the sample hole. The chip had 8 sample channels that were able to separate the different targets for simultaneous detection.…”

Section: Lateral Flow Technologymentioning

confidence: 99%

“…It remains a challenge to differentiate the fluorescent signals generated by CRISPR-based biosensors in the detection of multiple targets. Wu et al proposed an automated and multiplexed CRISPR-Cas12a ratiometric biochip sensor (RatioCRISPR) to detect the heteroplasmy level of mtDNA diseases . DNA was extracted and mixed with RPA reagent and then added to the chip from the sample hole.…”

Section: Strategies For Multiplexed Detection By Crispr-cas Systemmentioning

confidence: 99%

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