Ratio of mRNA expression of progesterone receptor isoforms AB is to b in human oviduct mucosal cells during the ovulatory cycle

Briton-Jones, Christine; Lok, Ingrid Hung; Cheung, Che Kwok; Po, A.L.S.; Chiu, Tony Tak Yu; Haines, Christopher J.

doi:10.1007/s10815-005-7203-5

Cited by 7 publications

(7 citation statements)

References 7 publications

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“…The finding that PR mRNA is lowest during the luteal phase (in both carriers and controls) is consistent with previous studies of normal human FTE throughout the ovarian cycle, which have shown little or no PR expression during this phase as determined by semi-quantitative RT-PCR ( Briton-Jones et al . 2005 ).…”

Section: Discussionsupporting

confidence: 91%

Decreased progesterone receptor isoform expression in luteal phase fallopian tube epithelium and high-grade serous carcinoma

Tone

Virtanen²,

Shaw³

et al. 2011

Endocrine Related Cancer

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We previously reported that BRCA1/2-mutated fallopian tube epithelium (FTE) collected during the luteal phase exhibits gene expression profiles more closely resembling that of high-grade serous carcinoma (HGSC) specimens than FTE collected during the follicular phase or from control patients. Since the luteal phase is characterised by high levels of progesterone, we determined whether the expression of progesterone receptor (PR) and PR-responsive genes was altered in FTE obtained from BRCA mutation carriers during the luteal phase of the menstrual cycle. RT-qPCR confirmed a decreased expression of PR mRNA in FTE during the luteal phase relative to follicular phase, in both BRCA1/2 mutation carriers and control patients. Immunohistochemistry using isoform-specific antibodies confirmed a low level of both PR-A and PR-B in HGSC and a lower level of staining in FTE samples obtained during the luteal phase compared with the follicular phase. No significant difference in PR-A or PR-B staining was found based on patient BRCA mutation status. Analysis of our previously reported gene expression profiles based upon known PR-A- and PR-B-specific target genes did not partition samples by BRCA mutation status, indicating that overall FTE PR response is not altered in BRCA mutation carriers. HGSC samples grouped separately from other samples, consistent with the observed loss of PR expression. These findings indicate no overall difference in PR signalling in FTE as a function of BRCA mutation status. Thus, the molecular similarity of BRCA1/2-mutated luteal phase FTE and HGSC likely results from an altered response to luteal phase factors other than progesterone.

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Section: Discussionsupporting

confidence: 91%

Decreased progesterone receptor isoform expression in luteal phase fallopian tube epithelium and high-grade serous carcinoma

Tone

Virtanen²,

Shaw³

et al. 2011

Endocrine Related Cancer

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“…increased progesterone receptor B in the ampulla) and are cycle-dependent (e.g. decreased mRNA of both receptors in the mid-luteal phase compared with the follicular phase) (Briton-Jones et al , 2005). Progesterone receptors A and B have been found in nuclei of all oviductal epithelial cells and in about half of stromal and smooth muscle cells (Horne et al , 2009), supporting the importance of progesterone signalling in this tissue and cell type-specific responses.…”

Section: Discussionmentioning

confidence: 99%

The human oviduct transcriptome reveals an anti-inflammatory, anti-angiogenic, secretory and matrix-stable environment during embryo transit

Hess

Talbi

Hamilton

et al. 2013

Reproductive BioMedicine Online

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The human oviduct serves as a conduit for spermatozoa in the periovulatory phase and nurtures and facilitates transport of the developing embryo for nidation during the luteal phase. Interactions between the embryo and oviductal epithelial surface proteins and secreted products during embryo transit are largely undefined. This study investigated gene expression in the human oviduct in the early luteal versus follicular phases to identify candidate genes and biomolecular processes that may participate in maturation and transport of the embryo as it traverses this tissue. Oviductal RNA was hybridized to oligonucleotide arrays and resulting data were analysed by bioinformatic approaches. There were 650 genes significantly down-regulated and 683 genes significantly up-regulated (P < 0.05) in the luteal versus follicular phase. Quantitative real-time PCR, immunoblot analysis and immunohistochemistry confirmed selected gene expression and cellular protein localization. Down-regulated genes involved macrophage recruitment, immunomodulation and matrix-degeneration, and up-regulated genes involved anti-inflammatory, ion transport, anti-angiogenic and early pregnancy recognition. The oviduct displayed some similarities and differences in progesterone-regulated genes compared with the human endometrium. Together, these data suggest a unique hormonally regulated environment during embryo development, maturation and transport through human oviduct and some conservation of progesterone signalling in tissues of common embryological origin.

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“…PGR is an important factor in the normal development and function of the reproductive tract (Bagchi et al, 2005;Briton-Jones et al, 2005Schams et al, 2003, mammary glands (Schams et al, 2003), and even reproductive behavior (Condon et al, 2003;Auger, 2004). Changes in the expression of PR-B and PR-A have been reported in reproductive tract malignancies such as endometrial (Sasaki et al, 2001) and ovarian tumors (Akahira et al, 2000) as well as in breast cancer (Lapidus RG et al, 1996).…”

Section: Implications Of This Studymentioning

confidence: 99%

The human progesterone receptor shows evidence of adaptive evolution associated with its ability to act as a transcription factor

Chen

Opazo²,

Erez³

et al. 2008

Molecular Phylogenetics and Evolution

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The gene encoding the progesterone receptor (PGR) acts as a transcription factor, and participates in the regulation of reproductive processes including menstruation, implantation, pregnancy maintenance, parturition, mammary development, and lactation. Unlike other mammals, primates do not exhibit progesterone withdrawal at the time of parturition. Because progesterone-mediated reproductive features vary among mammals, PGR is an attractive candidate gene for studies of adaptive evolution. Thus, we sequenced the progesterone receptor coding regions in a diverse range of species including apes, Old World monkeys, New World monkeys, prosimian primates, and other mammals. Adaptive evolution occurred on the human and chimpanzee lineages as evidenced by statistically significant increases in nonsynonymous substitution rates compared to synonymous substitution rates. Positive selection was rarely observed in other lineages. In humans, amino acid replacements occurred mostly in a region of the gene that has been shown to have an inhibitory function (IF) on the ability of the progesterone receptor to act as a transcription factor. Moreover, many of the nonsynonymous substitutions in primates occurred in the N-terminus. This suggests that cofactor interaction surfaces might have been altered, resulting in altered progesterone-regulated gene transcriptional effects. Further evidence that the changes conferred an adaptive advantage comes from SNP analysis indicating only one of the IF changes is polymorphic in humans. In chimpanzees, amino acid changes occurred in both the inhibitory and transactivation domains. Positive selection provides the basis for the hypothesis that changes in structure and function of the progesterone receptor during evolution contribute to the diversity of primate reproductive biology, especially in parturition.

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Ratio of mRNA expression of progesterone receptor isoforms AB is to b in human oviduct mucosal cells during the ovulatory cycle

Cited by 7 publications

References 7 publications

Decreased progesterone receptor isoform expression in luteal phase fallopian tube epithelium and high-grade serous carcinoma

Decreased progesterone receptor isoform expression in luteal phase fallopian tube epithelium and high-grade serous carcinoma

The human oviduct transcriptome reveals an anti-inflammatory, anti-angiogenic, secretory and matrix-stable environment during embryo transit

The human progesterone receptor shows evidence of adaptive evolution associated with its ability to act as a transcription factor

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