Ratio fluorescent hybrid probe for visualized fluorescence detection of H2O2 in vitro and in vivo

Dong, Riyue; Yao, Yuying; Li, Dongna; Zhang, Haoran; Li, Wei; Molokee, Maxim; Liu, Yingliang; Lei, Bingfu

doi:10.1016/j.snb.2020.128643

Cited by 42 publications

(12 citation statements)

References 60 publications

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“…The four peaks observed at 168.3, 286, 379.4, and 531.7 eV correspond to S 2p, C 1s, N 1s, and O 1s (Figure b). The strong binding energy peak at 284.7 eV (C–C) belongs to the CD-graphitized S 2p carbon structure (Figure c) . Subsequently, the optical properties of the CDs were fully investigated.…”

Section: Resultsmentioning

confidence: 99%

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Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

Wang

Kang

et al. 2021

ACS Omega

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With global warming, plants often suffer damage from high temperatures during the growth process, which inhibits their growth. In this work, carbon dots (CDs), synthesized by Salvia miltiorrhiza (S. miltiorrhiza) with a one-step hydrothermal method, were selected as heat-resistant enhancement agents for plants. Inspired by this background, this work studied Italian lettuce grown at 25, 35, and 45 °C and treated with CD and deionized water control (sprayed on leaves). The results showed that the biomass, chlorophyll content, net photosynthetic rate, activities of SOD (superoxide dismutase), POD (peroxidase), CAT (catalase), soluble sugar, and soluble protein contents of lettuce treated by CDs were increased while the contents of malondialdehyde (MDA) and proline (Pro) were decreased at 35 and 45 °C. The application of CDs at 35 and 45 °C could maintain the growth of plants by reducing oxidative damage and lipid peroxidation especially at the temperature of 35 °C, the growth status of lettuce treated by CDs was no different from that of lettuce grown naturally at the optimal temperature of 25 °C, or even better than the latter. This finding verified that the CDs could significantly improve the high-temperature tolerance of lettuce, thus alleviating the heat stress of plants.

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Section: Resultsmentioning

confidence: 99%

“…The strong binding energy peak at 284.7 eV (C−C) belongs to the CD-graphitized S 2p carbon structure (Figure 2c). 30 Subsequently, the optical properties of the CDs were fully investigated.…”

Section: Resultsmentioning

confidence: 99%

Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

Wang

Kang

et al. 2021

ACS Omega

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“…[32,33] Si QDs have attracted considerable attention in chemistry and biology because of their low toxicity, excellent water solubility, good sta bility, and ease of synthesis. [34][35][36][37] However, the development of new QD probes from the combination of Si QDs with other QDs is less common. The Si and CdTe QDs are developed with core-shell structures in recent years.…”

Section: Introductionmentioning

confidence: 99%

“…[12][13][14][15] Fluorescent probes are ideal for detecting analytes in biological sam ples because of their fast response, high selectivity, good compatibility with biolog ical samples, and nondestructive analysis capability. [16][17][18] Dong et al [19] invented a silicon oxide quantum dots (SiO QDs) and Ag nanoclusters hybrid nanosensing probe that acts as an "offon" ratio fluo rescent probe for H 2 O 2 detection. Gong et al [20] developed a H 2 O 2 probe based on a hepatitis B core antibody labeled with horseradish peroxidase (HBcAbHRP) induced fluorescent enhancement of CdTe QDs.…”

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confidence: 99%

A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H₂O₂

Zhou

Zhao

et al. 2022

Adv Funct Materials

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Hydrogen peroxide is an important reactive oxygen species (ROS) that acts as a significant marker of oxidative stress in certain diseases. Owing to the autofluorescence of biological samples, H 2 O 2 monitoring remains challenging. Here, Si-CdTe quantum dots (QDs) are prepared as novel ratiometric fluorescent probes prepared for sensitive detection of H 2 O 2 and glucose, the low detection limit of H 2 O 2 and glucose is 79 and 140 nm, respectively. In this article, CdTe QDs are combined with Si QDs to reduce the cytotoxicity of CdTe. The survival rate of HeLa cells remains at 80% after incubation with the Si-CdTe QDs at a concentration of 500 µg mL −1 for 24 h. Fluorescence extinguishes based on 488 and 514 nm channels are used as internal references to avoid environmental interference and demonstrate selective H 2 O 2 biosensing. In addition, the probe has an excellent colocalization effect in the lysosome under the 514 nm channel, with a maximum Pearson coefficient of 0.96. The advantages of easy preparation, low toxicity, high resistance to photobleaching, and high specificity of the Si-CdTe QDs probes provide a new and convenient means to monitor and detect changes in the endogenous intracellular H 2 O 2 concentration and to select 514 nm lysosomal colocalization dyes.

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“…A hybrid nano-sensing probe consisting of silicon oxide quantum dots (Si–O QDs) and silver nanoclusters (Ag NCs) has been used to measure H 2 O 2 directly in Lettuce mitochondria in vitro , and in vivo imaging of H 2 O 2 was also reported following wounding. 38 The intrinsic fluorescence of the Ag NCs is quenched by H 2 O 2 thus can act as a signal for H 2 O 2 detection, and while the Si–O QD signal is also quenched, it gradually recovers whereas that of the Ag-NCs decreases at two different wavelengths, meaning the fluorescence ratio can be used to quantify H 2 O 2 accurately. Unfortunately, for this nanohybrid probe, in vivo chloroplast fluorescence overlapped with Ag NC fluorescence, meaning a two-signal ratio measurement was not attainable for green tissue, although this approach could certainly be developed.…”

Section: Direct Measurement Of Ros Species Using Chemical Probes Biosensors and Electron Paramagnetic Resonancementioning

confidence: 99%

Measuring ROS and redox markers in plant cells

et al. 2021

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Ratio fluorescent hybrid probe for visualized fluorescence detection of H2O2 in vitro and in vivo

Cited by 42 publications

References 60 publications

Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H₂O₂

Measuring ROS and redox markers in plant cells

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Ratio fluorescent hybrid probe for visualized fluorescence detection of H2O2 in vitro and in vivo

Cited by 42 publications

References 60 publications

Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H2O2

Measuring ROS and redox markers in plant cells

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Product

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About

A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H₂O₂