Ratio [13C]/[12C] as an index for express estimation of hydrocarbon-oxidizing potential of microbiota in soil polluted with crude oil

Zyakun, A. M.; Boronin, A. M.; Кочетков, В. В.; Баскунов, Б. П.; Лауринавичус, К. С.; Zakharchenko, V. N.; Peshenko, V. P.; Anokhina, Tatyana S.; Сиунова, Т. В.

doi:10.1134/s0003683812020159

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…The -3.5% decrease in petroleum hydrocarbons observed after bioremediation was apparently associated with the discharge of volatile compounds and CO 2 produced by petroleum oxidiz ing microorganisms. A similar level of microbial oil mineralization in soil was observed in model experi ments [18].…”

Section: Organic and Mineral Components Of The Oil Sludgesupporting

confidence: 78%

“…A consid erable decrease in SOM content in the oil sludge observed after bioremediation (by -7%) was appar ently associated with the priming effect [18], which means that microbial oxidation of soil derived organic compounds can be activated by the consumption of petroleum hydrocarbons.…”

Section: Organic and Mineral Components Of The Oil Sludgementioning

confidence: 94%

“…It was previously shown that the ratio of stable car bon isotope distribution ( 13 С/ 12 С) is different for hydrocarbons derived from oil and from soil organic matter (SOM); thus, it can be used as a natural isotope label [18]. Since the isotope composition of oil derived carbon is different from that of SOM carbon, it should be possible to quantify residual petroleum hydrocarbons, as well as hydrocarbons transformed by microorganisms into organic compounds and SOM in petroleum contaminated soils, based on the analysis of carbon isotope composition.…”

Section: Bioremediation Of Oil Polluted Soils: Using the [ 13 C]/[ 12mentioning

confidence: 99%

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Bioremediation of oil-polluted soils: Using the [13C]/[12C] ratio to characterize microbial products of oil hydrocarbon biodegradation

Zyakun

Бродский

Баскунов

et al. 2014

Appl Biochem Microbiol

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We compared data on the extent of bioremediation in soils polluted with oil. The data were obtained using conventional methods of hydrocarbon determination: extraction gas chromatography-mass spectrometry, extraction IR spectroscopy, and extraction gravimetry. Due to differences in the relative abun dances of the stable carbon isotopes ( 13 C/ 12 C) in oil and in soil organic matter, these ratios could be used as natural isotopic labels of either substance. Extraction gravimetry in combination with characteristics of the carbon isotope composition of organic products in the soil before and after bioremediation was shown to be the most informative approach to an evaluation of soil bioremediation. At present, it is the only method enabling quantification of the total petroleum hydrocarbons in oil polluted soil, as well as of the amounts of hydrocarbons remaining after bioremediation and those microbially transformed into organic products and biomass.

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Section: Organic and Mineral Components Of The Oil Sludgesupporting

confidence: 78%

Section: Organic and Mineral Components Of The Oil Sludgementioning

confidence: 94%

Section: Bioremediation Of Oil Polluted Soils: Using the [ 13 C]/[ 12mentioning

confidence: 99%

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Bioremediation of oil-polluted soils: Using the [13C]/[12C] ratio to characterize microbial products of oil hydrocarbon biodegradation

Zyakun

Бродский

Баскунов

et al. 2014

Appl Biochem Microbiol

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“…This short-term to long-term ratio increased linearly from 1.7 for C 15 to 3.0 for C 40 (Fig. S4b) suggesting a priming effect 35,36 of the lighter hydrocarbons in the degradation process of the heavier compounds in the first half year of the experiment.…”

Section: Resultsmentioning

confidence: 88%

Decomposition of sediment-oil-agglomerates in a Gulf of Mexico sandy beach

Bociu

Shin

Wells

et al. 2019

Sci Rep

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Sediment-oil-agglomerates (SOA) are one of the most common forms of contamination impacting shores after a major oil spill; and following the Deepwater Horizon (DWH) accident, large numbers of SOAs were buried in the sandy beaches of the northeastern Gulf of Mexico. SOAs provide a source of toxic oil compounds, and although SOAs can persist for many years, their long-term fate was unknown. Here we report the results of a 3-year in-situ experiment that quantified the degradation of standardized SOAs buried in the upper 50 cm of a North Florida sandy beach. Time series of hydrocarbon mass, carbon content, n-alkanes, PAHs, and fluorescence indicate that the decomposition of golf-ball-size DWH-SOAs embedded in beach sand takes at least 32 years, while SOA degradation without sediment contact would require more than 100 years. SOA alkane and PAH decay rates within the sediment were similar to those at the beach surface. The porous structure of the SOAs kept their cores oxygen-replete. The results reveal that SOAs buried deep in beach sands can be decomposed through relatively rapid aerobic microbial oil degradation in the tidally ventilated permeable beach sand, emphasizing the role of the sandy beach as an aerobic biocatalytical reactor at the land-ocean interface.

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“…It has been demonstrated that addition of exogenous organic substrate (e.g., glucose, cellulose, amino acids, etc.) to soil has caused either inhibition or activation of microbial SOM mineralization as compared to the untreated soil, which became known as negative or positive priming effects of SOM, respectively . Previously, Zyakun et al demonstrated that alkane addition to soil provoked positive SOM priming.…”

Section: Introductionmentioning

confidence: 99%

Microbial Activity and Decomposition of Soil Organic Matter in Roadside Soils Contaminated With Petroleum Hydrocarbons

Mykhailova

Fischer

Iurchenko³

2018

CLEAN Soil Air Water

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Petroleum hydrocarbons (PHs) are the most widespread organic pollutants of roadside soils, the reclamation of which is costly due to large areas affected, and natural PHs biodegradation is the only way of mitigating negative ecological consequences. This study aimed to investigate the microbial response to PHs in roadside soils and to identify soil organic matter (SOM) and PHs involved in biodegradation processes. To address this aim, soil respiration is related to light (chain‐length ≤C14) medium (C15–C27), and heavy fractions (≥C28) of total PHs, and to SOM components: carbohydrates, proteins, lignin, aliphates, carbonyl/carboxyl, and char amounts of soils sampled at distances of up to 100 m from three roads in Kharkiv, Ukraine. A close relation between soil respiration and ratio of the light fraction to the sum of medium and heavy alkane fractions indicate the preferential mineralization of more biodegradable light PHs fractions. A positive relation between soil respiration and carbohydrate C, as well as negative correlation with aliphatic C, indicate preferred degradation of carbohydrates compared to more recalcitrant aliphates. Carbohydrate content increased and aliphatic C decreased with distance from the roads, and carbohydrates decreased with PHs concentration, indicating increased degradation of carbohydrates at high PHs concentrations (positive priming). It is concluded that microorganisms generate the energy required for PHs degradation by enhanced oxidation of native SOM where carbohydrates were of paramount importance.

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Ratio [13C]/[12C] as an index for express estimation of hydrocarbon-oxidizing potential of microbiota in soil polluted with crude oil

Cited by 8 publications

References 18 publications

Bioremediation of oil-polluted soils: Using the [13C]/[12C] ratio to characterize microbial products of oil hydrocarbon biodegradation

Bioremediation of oil-polluted soils: Using the [13C]/[12C] ratio to characterize microbial products of oil hydrocarbon biodegradation

Decomposition of sediment-oil-agglomerates in a Gulf of Mexico sandy beach

Microbial Activity and Decomposition of Soil Organic Matter in Roadside Soils Contaminated With Petroleum Hydrocarbons

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